IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ goodchild-2005-1 The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Goodchild RE., Dauer WT. J. Cell Biol. (0021-9525) 2005 goodchild-2005-2 The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Goodchild RE., Dauer WT. J. Cell Biol. (0021-9525) 2005 lrrc7_human Leucine-rich repeat-containing protein 7 Protein LAP1 Densin-180 LRRC7 KIAA1365 LAP1 protein protein human Homo sapiens Human MTTKRKIIGRLVPCRCFRGEEEIISVLDYSHCSLQQVPKEVFNFERTLEELYLDANQIEELPKQLFNCQALRKLSIPDNDLSNLPTTIASLVNLKELDISKNGVQEFPENIKCCKCLTIIEASVNPISKLPDGFTQLLNLTQLYLNDAFLEFLPANFGRLVKLRILELRENHLKTLPKSMHKLAQLERLDLGNNEFGELPEVLDQIQNLRELWMDNNALQVLPGSIGKLKMLVYLDMSKNRIETVDMDISGCEALEDLLLSSNMLQQLPDSIGLLKKLTTLKVDDNQLTMLPNTIGNLSLLEEFDCSCNELESLPSTIGYLHSLRTLAVDENFLPELPREIGSCKNVTVMSLRSNKLEFLPEEIGQMQKLRVLNLSDNRLKNLPFSFTKLKELAALWLSDNQSKALIPLQTEAHPETKQRVLTNYMFPQQPRGDEDFQSDSDSFNPTLWEEQRQQRMTVAFEFEDKKEDDENAGKVKDLSCQAPWERGQRGITLQPARLSGDCCTPWARCDQQIQDMPVPQNDPQLAWGCISGLQQERSMCTPLPVAAQSTTLPSLSGRQVEINLKRYPTPYPEDLKNMVKSVQNLVGKPSHGVRVENSNPTANTEQTVKEKYEHKWPVAPKEITVEDSFVHPANEMRIGELHPSLAETPLYPPKLVLLGKDKKESTDESEVDKTHCLNNSVSSGTYSDYSPSQASSGSSNTRVKVGSLQTTAKDAVHNSLWGNRIAPSFPQPLDSKPLLSQREAVPPGNIPQRPDRLPMSDTFTDNWTDGSHYDNTGFVAEETTAENANSNPLLSSKSRSTSSHGRRPLIRQDRIVGVPLELEQSTHRHTPETEVPPSNPWQNWTRTPSPFEDRTAFPSKLETTPTTSPLPERKEHIKESTEIPSPFSPGVPWEYHDSNPNRSLSNVFSQIHCRPESSKGVISISKSTERLSPLMKDIKSNKFKKSQSIDEIDIGTYKVYNIPLENYASGSDHLGSHERPDKMLGPEHGMSSMSRSQSVPMLDDEMLTYGSSKGPQQQKASMTKKVYQFDQSFNPQGSVEVKAEKRIPPPFQHNPEYVQQASKNIAKDLISPRAYRGYPPMEQMFSFSQPSVNEDAVVNAQFASQGARAGFLRRADSLVSATEMAMFRRVNEPHELPPTDRYGRPPYRGGLDRQSSVTVTESQFLKRNGRYEDEHPSYQEVKAQAGSFPVKNLTQRRPLSARSYSTESYGASQTRPVSARPTMAALLEKIPSDYNLGNYGDKPSDNSDLKTRPTPVKGEESCGKMPADWRQQLLRHIEARRLDRNAAYKHNTVNLGMLPYGGISAMHAGRSMTLNLQTKSKFDHQELPLQKTPSQQSNILDNGQEDVSPSGQWNPYPLGRRDVPPDTITKKAGSHIQTLMGSQSLQHRSREQQPYEGNINKVTIQQFQSPLPIQIPSSQATRGPQPGRCLIQTKGQRSMDGYPEQFCVRIEKNPGLGFSISGGISGQGNPFKPSDKGIFVTRVQPDGPASNLLQPGDKILQANGHSFVHMEHEKAVLLLKSFQNTVDLVIQRELTV tor1a_human Torsin-1A Torsin family 1 member A Dystonia 1 protein TOR1A DQ2 DYT1 TA Torsin ATPase-1A TORA protein protein human Homo sapiens Human MKLGRAVLGLLLLAPSVVQAVEPISLGLALAGVLTGYIYPRLYCLFAECCGQKRSLSREALQKDLDDNLFGQHLAKKIILNAVFGFINNPKPKKPLTLSLHGWTGTGKNFVSKIIAENIYEGGLNSDYVHLFVATLHFPHASNITLYKDQLQLWIRGNVSACARSIFIFDEMDKMHAGLIDAIKPFLDYYDLVDGVSYQKAMFIFLSNAGAERITDVALDFWRSGKQREDIKLKDIEHALSVSVFNNKNSGFWHSSLIDRNLIDYFVPFLPLEYKHLKMCIRVEMQSRGYEIDEDIVSRVAEEMTFFPKEERVFSDKGCKTVFTKLDYYYDD toip2_human Torsin-1A-interacting protein 2 Lumenal domain-like LAP1 TOR1AIP2 LULL1 IFRG15 protein protein human Homo sapiens Human MADSGLREPQEDSQKDLENDPSVNSQAQETTIIASNAEEAEILHSACGLSKDHQEVETEGPESADTGDKSESPDEANVGKHPKDKTEDENKQSFLDGGKGHHLPSENLGKEPLDPDPSHSPSDKVGRADAHLGSSSVALPKEASDGTGASQEPPTTDSQEAQSPGHSSAGQEGEDTLRRRLLAPEAGSHPQQTQKLEEIKENAQDTMRQINKKGFWSYGPVILVVLVVAVVASSVNSYYSSPAQQVPKNPALEAFLAQFSQLEDKFPGQSSFLWQRGRKFLQKHLNASNPTEPATIIFTAAREGRETLKCLSHHVADAYTSSQKVSPIQIDGAGRTWQDSDTVKLLVDLELSYGFENGQKAAVVHHFESFPAGSTLIFYKYCDHENAAFKDVALVLTVLLEEETLEASVGPRETEEKVRDLLWAKFTNSDTPTSFNHMDSDKLSGLWSRISHLVLPVQPVSSIEEQGCLF tor1a-lrrc7-1 Co-IP: prey = LAP-1, bait = mutated E303Q Torsin A 175494 N/A 175496 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 175497 unspecified role unspecified role prey prey gfp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O14656:p.Glu303Gln necessary mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The mutation E303Q is closed to the mutation found in TORSION DYSTONIA 1 disease which is often a deletion of E303 (glutamic acid deletion). Both mutated proteins are mislocalized in the nuclear envelope lumen instead of the endoplasmic reticulum. The mutated E303Q Torsin A can be detected in LAP-1 immunoprecipitates but not the WT Torsin A. tor1a-q8nfq8-1 Co-IP: prey = LULL1, bait = mutated E303Q Torsin A 175494 N/A 175498 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 175497 unspecified role unspecified role prey prey O14656:p.Glu303Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false gfp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false The mutation E303Q is closed to the mutation found in TORSION DYSTONIA 1 disease which is often a deletion of E303 (glutamic acid deletion). Both mutated proteins are mislocalized in the nuclear envelop lumen instead of the endoplasmic reticulum. LULL1 immunoprecipitates more mutated E303Q Torsin A than WT Torsin A. tor1a-lrrc7-2 Fluorescent microscopy : LAP-1 recruits mutated Torsin A to the nuclear envelope 175495 N/A 175497 unspecified role unspecified role neutral component neutral component O14656:p.Glu303Gln necessary mutation mutation certain certain sequence position certain certain certain sequence position certain false gfp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 175496 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false In TORSION DYSTONIA 1 disease, a deletion of E303 (glutamic acid deletion) of Torsin A is often found. The mutated protein is mislocalized in the nuclear envelope lumen instead of the endoplasmic reticulum. Overexpression of LAP-1 changes Torsin A WT localization from the endoplasmic reticulum to the nuclear envelope