UniProt UniProt http://www.uniprot.org tops-2005-2 RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Yeast strain AH109 (Clontech) containing GAL4 dependent reporter genes HIS3, ADE2, and LacZ. MUT7-GAL4 DNA binding domain fusion expressed from the pGBKT7 vector (Clontech). Mixed stage C.elegans poly(A) cDNA library in pACT vector. Nucleic Acids Res. (0305-1048) 2005 IMEx Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF., Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Only protein-protein interactions imex curation imex curation tops-2005-3 RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Yeast strain AH109 (Clontech) containing GAL4 dependent reporter genes HIS3, ADE2, and LacZ. MUT7-GAL4 DNA binding domain fusion expressed from the pGBKT7 vector (Clontech). Mixed stage C.elegans poly(A) cDNA library in pACT vector. Nucleic Acids Res. (0305-1048) 2005 IMEx Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF., Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Only protein-protein interactions imex curation imex curation tops-2005-5 caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Yeast strain AH109 (Clontech) containing GAL4 dependent reporter genes HIS3, ADE2, and LacZ. MUT7-GAL4 DNA binding domain fusion expressed from the pGBKT7 vector (Clontech). Mixed stage C.elegans poly(A) cDNA library in pACT vector. Nucleic Acids Res. (0305-1048) 2005 IMEx Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF., Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Only protein-protein interactions imex curation imex curation tops-2005-1 RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Yeast strain AH109 (Clontech) containing GAL4 dependent reporter genes HIS3, ADE2, and LacZ. MUT7-GAL4 DNA binding domain fusion expressed from the pGBKT7 vector (Clontech). Mixed stage C.elegans poly(A) cDNA library in pACT vector. Nucleic Acids Res. (0305-1048) 2005 IMEx Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF., Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Only protein-protein interactions imex curation imex curation tops-2005-4 RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Yeast strain AH109 (Clontech) containing GAL4 dependent reporter genes HIS3, ADE2, and LacZ. MUT7-GAL4 DNA binding domain fusion expressed from the pGBKT7 vector (Clontech). Mixed stage C.elegans poly(A) cDNA library in pACT vector. Nucleic Acids Res. (0305-1048) 2005 IMEx Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF., Tops BB., Tabara H., Sijen T., Simmer F., Mello CC., Plasterk RH., Ketting RF. Only protein-protein interactions imex curation imex curation q19672_caeel rde-2 F21C3.4 CELE_F21C3.4 protein protein caeel Caenorhabditis elegans MHNGYHSYFPPNHYYAQSQPSSSYNPQPQIQQPQTQIYGLVVGYNKYGNRNYLFYTKNIDGFVEISGNIQSLQIGRWLKLTVCQNFVGQYVLDSSNSCGYENWDVLPNHRNSSARFFPKYEANDEGEILITARFDVIVDRKNNLYDFTSYDISYDHIIDDFSLIKSSCQHYDGRSFIVEAQASLAGWVALSVHEDPQKLHTCDAVSRNGYQSLPNPVVDSDVQYLHDTRLLDIMTGNGYVQTPETDVQDQQSSQHQEDVHSQMNSQTSDSYNSSRVVSENREIPPETFRSQSIELNQVDSSLSQTTISSRAAPVIDSNQLSDEDEIDDEDTYGTRGTSNIPMRPFIKDLAPTMLQLLRQDKTDSEKPQSALCTVVQKIDGFAILYTAKRDVINVLLQERSCEGLERSPQLGDVAFFDILPRRIETKDRLIFKIPYTHIAVKKKPDTPDSLLKIDCFKNSVRCFGGVLEMKVKIALSKPELVVEQYHDNTEMNSDHHFYYLKATNGVLVTIPKERLLNHLNSKLSADFDLIAWVVHRKPIGNVSLHIGKGGEAYQQFTNGDIRELPPLSSNQYFMNVRK mut7_caeel Exonuclease mut-7 mut-7 ZK1098.8 Exonuclease 3'-5' domain-containing protein 3 homolog protein protein caeel Caenorhabditis elegans MEEEPYKRKLTKAEKKAKYRTDYAEPLKSRREVLKAIMNGPESERERKVRAKNREFFNEDYRSGVNIYGMAVDMMKAMPDRGKTSGQSLAVWYLEDFGVWLKESGQETELRQKYLTGTIQINALDVCTIGQKQLLSEIFDITKEKFTEDITQLLDAAIKKQDFSVAADMAIQYNLLRDHHFEHLVLPLMLSGKDQTAYKLISNNERMQQQLVEFFDRMVGISVVAVEEMLKPYKETKIMTIPMEKLTGKTLDKLISTIINKNTHEYNFSRELSKFAKNHSQNGNLKALKFNISERYEKGKSDDNYFQHMVETFTKAEDVREPILFYLWSSNDTEKQIDAICFAIYLGIASSSSYQLPNVMRDFFRQPDSKLREAKELLVRRKTLQVPLNGEQLFVFENERRTQIHMVKTESEMNYLCSEIKSLSDEPAPVYVGFDSEWKPSNLTAVHDSKIAIIQLFFKNCVWLVDCVELEKANMADDWWQKFASRLFGDSPVKVVGFDMRNDLDAMATIPALKSSMKIEDTKNAFDLKRLAENVCDIDMEILELPKKTFKLADLTHYLLGLELDKTEQCSNWQCRPLRKKQIVYAALDAVVVVETFKKILSIVEEKNKDADIEKIVRESNVMAPKKDKGHKSYRKLKTIPWLELYDILRSHRNPTRSPQRPHDIKVIVDTMLIGFGKNLRRVGIDVILPKDVSDFRKYLKEIERVGGEHLRHIITVPSKSYEALKMDYDNYTIAIPELNNMSPVDQLIEFFDLFNVDIRPEDVYPRCTECNSRLQIKFPGPVLHFLHQYCVIHVQNVYRADMSEFPLEEWWNRMLHINPDDYDGVKVEMSRPSPTSKWIVATVPTGCLHITRQTALHTNLPDGIEVRIHKVPDDEFKRRNLSFYVCGECGTVACDGRGNQASESTSQEC mut7-f21c3_4-2 MUT7-F21C3.4 interaction by Y2H. 169004 N/A 169009 unspecified role unspecified role bait bait N/A 169010 unspecified role unspecified role prey prey physical association physical association false false false f21c3_4-f21c3_4-1 F21C3.4 homotypic interaction by Y2H. 169004 N/A 169009 unspecified role unspecified role bait bait N/A 169009 unspecified role unspecified role prey prey for f21c3_4 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f21c3_4-mut7-2 MUT7-F21C3.4 interaction by Y2H. 169005 N/A 169009 unspecified role unspecified role bait bait for mut7 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 169010 unspecified role unspecified role prey prey physical association physical association false false false Fig 3A mut7-f21c3_4-4 MUT7-F21C3.4 interaction by Y2H. 169005 N/A 169009 unspecified role unspecified role bait bait N/A 169010 unspecified role unspecified role prey prey for f21c3_4 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3A mut7-f21c3_4-6 MUT7-F21C3.4 interaction by Y2H. 169006 N/A 169010 unspecified role unspecified role prey prey N/A 169009 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4D mut7-f21c3_4-1 MUT7-F21C3.4 interaction by Y2H. 169007 N/A 169010 unspecified role unspecified role bait bait N/A 169009 unspecified role unspecified role prey prey region Minimal domain necessary for MUT7 interaction identified in Y2H. sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false mut7-f21c3_4-7 MUT7-F21C3.4 interaction by re-localization. 169008 N/A 169010 unspecified role unspecified role neutral component neutral component tmd-tag n-terminus tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 169009 unspecified role unspecified role neutral component neutral component n-terminal gfp-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Expression of MUT7 protein tagged with a transmembrane domain (TMD) induces relocalization of GFP-tagged F21C3.4 protein to the membrane regions of body wall muscle cells.