UniProt UniProt http://www.uniprot.org costa-2005-1 Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Costa R., Warren DT., Ayscough KR. Biochem. J. (0264-6021) 2005 costa-2005-2 Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Costa R., Warren DT., Ayscough KR. Biochem. J. (0264-6021) 2005 costa-2005-3 Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Costa R., Warren DT., Ayscough KR. Biochem. J. (0264-6021) 2005 costa-2005-4 Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Costa R., Warren DT., Ayscough KR. Biochem. J. (0264-6021) 2005 lsb5_yeast LAS seventeen-binding protein 5 YCL186 YCL34W YCL034W LSB5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGFLSDHPHTAITETIFRIVSSRDYTLEVELAPLIQLIKADHNDYNYTVNQEEAARALRKKIKYGNRLQQSRTLDLLDLFISQGVKFTVMYNDDKLLQRLRGMATNSENSGSGEKYEPRIIKKCAAYAISWLNYITQNNLENARAYSGLYQLGQTVKQRYSKSSRSRRSGGGSGGRSNFMDDSADDTLYQSNSLTSADRLYRIPQINMNKEAPRIRLIISDALASAVSLQNSLIGLPKGKFSTDDEEATSKFIQARAIRRKVLRYLQLVTEGEFLGSLIHANDELVAALTAYDDRSAQDDSSDESDHGSYDDGIYDENEQDNSRYIDSESSEEESLSSYQPSTISNPFGDHNKI sla1_yeast Actin cytoskeleton-regulatory complex protein SLA1 YBL0321 YBL007C SLA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVFLGIYRAVYAYEPQTPEELAIQEDDLLYLLQKSDIDDWWTVKKRVIGSDSEEPVGLVPSTYIEEAPVLKKVRAIYDYEQVQNADEELTFHENDVFDVFDDKDADWLLVKSTVSNEFGFIPGNYVEPENGSTSKQEQAPAAAEAPAATPAAAPASAAVLPTNFLPPPQHNDRARMMQSKEDQAPDEDEEGPPPAMPARPTATTETTDATAAAVRSRTRLSYSDNDNDDEEDDYYYNSNSNNVGNHEYNTEYHSWNVTEIEGRKKKKAKLSIGNNKINFIPQKGTPHEWSIDKLVSYDNEKKHMFLEFVDPYRSLELHTGNTTTCEEIMNIIGEYKGASRDPGLREVEMASKSKKRGIVQYDFMAESQDELTIKSGDKVYILDDKKSKDWWMCQLVDSGKSGLVPAQFIEPVRDKKHTESTASGIIKSIKKNFTKSPSRSRSRSRSKSNANASWKDDELQNDVVGSAAGKRSRKSSLSSHKKNSSATKDFPNPKKSRLWVDRSGTFKVDAEFIGCAKGKIHLHKANGVKIAVAADKLSNEDLAYVEKITGFSLEKFKANDGSSSRGTDSRDSERERRRRLKEQEEKERDRRLKERELYELKKARELLDEERSRLQEKELPPIKPPRPTSTTSVPNTTSVPPAESSNNNNSSNKYDWFEFFLNCGVDVSNCQRYTINFDREQLTEDMMPDINNSMLRTLGLREGDIVRVMKHLDKKFGRENIASIPTNATGNMFSQPDGSLNVATSPETSLPQQLLPQTTSPAQTAPSTSAETDDAWTVKPASKSESNLLSKKSEFTGSMQDLLDLQPLEPKKAAASTPEPNLKDLEPVKTGGTTVPAAPVSSAPVSSAPAPLDPFKTGGNNILPLSTGFVMMPMITGGDMLPMQRTGGFVVPQTTFGMQSQVTGGILPVQKTGNGLIPISNTGGAMMPQTTFGAAATVLPLQKTGGGLIPIATTGGAQFPQTSFNVQGQQQLPTGSILPVQKTANGLISANTGVSMPTVQRTGGTMIPQTSFGVSQQLTGGAMMTQPQNTGSAMMPQTSFNAVPQITGGAMMPQTSFNALPQVTGGAMMPLQRTGGALNTFNTGGAMIPQTSFSSQAQNTGGFRPQSQFGLTLQKTGGIAPLNQNQFTGGAMNTLSTGGVLQQQQPQTMNTFNTGGVMQELQMMTTFNTGGAMQQPQMMNTFNTDGIMQQPQMMNTFNTGGAMQQPQQQALQNQPTGFGFGNGPQQSRQANIFNATASNPFGF 7FD85AA776407624 las17_yeast Proline-rich protein LAS17 LAS17 YOR181W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLLNSSDKEIIKRALPKASNKIIDVTVARLYIAYPDKNEWQYTGLSGALALVDDLVGNTFFLKLVDINGHRGVIWDQELYVNFEYYQDRTFFHTFEMEECFAGLLFVDINEASHFLKRVQKRERYANRKTLLNKNAVALTKKVREEQKSQVVHGPRGESLIDNQRKRYNYEDVDTIPTTKHKAPPPPPPTAETFDSDQTSSFSDINSTTASAPTTPAPALPPASPEVRKEETHPKHSLPPLPNQFAPLPDPPQHNSPPQNNAPSQPQSNPFPFPIPEIPSTQSATNPFPFPVPQQQFNQAPSMGIPQQNRPLPQLPNRNNRPVPPPPPMRTTTEGSGVRLPAPPPPPRRGPAPPPPPHRHVTSNTLNSAGGNSLLPQATGRRGPAPPPPPRASRPTPNVTMQQNPQQYNNSNRPFGYQTNSNMSSPPPPPVTTFNTLTPQMTAATGQPAVPLPQNTQAPSQATNVPVAPPPPPASLGQSQIPQSAPSAPIPPTLPSTTSAAPPPPPAFLTQQPQSGGAPAPPPPPQMPATSTSGGGSFAETTGDAGRDALLASIRGAGGIGALRKVDKSQLDKPSVLLQEARGESASPPAAAGNGGTPGGPPASLADALAAALNKRKTKVGAHDDMDNGDDW 4488355563AA2645 arf3_yeast ADP-ribosylation factor 3 ARF3 YOR3172W YOR094W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGNSISKVLGKLFGSKEMKILMLGLDKAGKTTILYKLKLNKIKTSTPTVGFNVETVTYKNVKFNMWDVGGQQRLRPLWRHYFPATTALIFVIDSSARNRMEEAKEELYSIIGEKEMENVVLLVWANKQDLKDAMKPQEVSDFLELEKNLKNQPWCVIGSNALSGQGLVEGLSWISNNTNVPKK ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein sla1-lsb5-1 Sla1 HD1-gap-HD2 region interaction by yeast two hybrid with Lsb5 c-terminus 169038 N/A 169042 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 169043 unspecified role unspecified role bait bait hd1-hd2 fragment 486 to 731 containing HD1 and HD2 domains binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false sla1-lsb5-5 Sla1 HD1 region interaction by yeast two hybrid with Lsb5 c-terminus 169038 N/A 169043 unspecified role unspecified role bait bait hd1 Homology domain 1 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 169042 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal npf Deletion of c-terminal NPF domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fragment 301-354 interaction with HD1 domain of Sla1 is weaker than for fragment 174-354 containing a GAT domain. The lsb5 C-terminal NPF domain is essential for interaction with sla1. sla1-lsb5-8 Sla1 HD1 region interaction by yeast two hybrid with Lsb5 c-terminus 169038 N/A 169042 unspecified role unspecified role prey prey c-terminal npf Deletion of c-terminal NPF domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminus binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 169043 unspecified role unspecified role bait bait hd1 Homology domain 1 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fragment 301-354 interaction with HD1 domain of Sla1 is weaker than for fragment 174-354 containing a GAT domain. The lsb5 C-terminal NPF domain is essential for interaction with sla1. lsb5-las17-2 Yeast two hybrid interaction between Lsb5p and Las17p. 169038 N/A 169044 unspecified role unspecified role prey prey las17-short-2 fragment 292-422 of Las17p binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 169042 unspecified role unspecified role bait bait physical association physical association false false false sla1-lsb5-7 Interaction shown by GST-pulldown 169039 N/A 169042 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 169043 unspecified role unspecified role prey prey hd1 Homology domain 1 binding region binding-associated region binding region polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false hd1 The HD1 fragment was HIS tagged for purification his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false The Sla1 HD1 domain alone is necessary and sufficient to support the interaction with Lsb5. lsb5-arf3-2 No interaction between Lsb5p and Arf3p (mutant T31N) detected by GST pull down 169039 N/A 169042 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 169045 unspecified role unspecified role prey prey P40994:p.Thr32Asn GTP binding defective mutation no more binds to lsb5p. mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false The arf3 T31N mutation abolishes the interaction with lsb5 sla1-lsb5-9 Interaction shown by GST-pulldown 169039 N/A 169043 unspecified role unspecified role prey prey hd1-hd2 The HD1-HD2 fragment was HIS tagged for purification his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false hd1-hd2 Homology domains 1 and 2 binding region binding-associated region binding region polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 169042 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false lsb5-arf3-1 Interaction between Lsb5p and Arf3p detected by GST pull down 169039 N/A 169042 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) N/A 169045 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false lsb5-las17-1 Yeast two hybrid interaction between Lsb5p and Las17p. 169040 N/A 169042 unspecified role unspecified role bait bait N/A 169044 unspecified role unspecified role prey prey las17-short-1 fragment 292-536 of Las17p binding region binding-associated region binding region full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false lsb5-ubi Interaction between Lsb5p and ubiquitin revealed by GST pull down 169041 N/A 169042 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) N/A 169046 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false