IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lillard_wet-2004-6 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-1 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-2 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. human-va13_2ra Homo sapiens Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) va13_2ra Human Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-3 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. human-va13_2ra Homo sapiens Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) va13_2ra Human Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) fluorescence imaging fluorescence microscopy fluorescence imaging western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-4 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma fluorescence imaging fluorescence microscopy fluorescence imaging western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-5 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 lillard_wet-2004-7 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Hum. Mol. Genet. (0964-6906) 2004 blm_human Bloom syndrome protein RecQ protein-like 3 DNA helicase, RecQ-like type 2 BLM RECQ2 RECQL3 protein protein human Homo sapiens Human MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS terf1_human Telomeric repeat-binding factor 1 TTAGGG repeat-binding factor 1 NIMA-interacting protein 2 Telomeric protein Pin2/TRF1 TERF1 PIN2 TRF TRF1 TRBF1 protein protein human Homo sapiens Human MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED terf2_human Telomeric repeat-binding factor 2 TTAGGG repeat-binding factor 2 Telomeric DNA-binding protein TERF2 TRF2 TRBF2 protein protein human Homo sapiens Human MAAGAGTAGPASGPGVVRDPAASQPRKRPGREGGEGARRSDTMAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTMAKKALKSESAASSTGKEDKQPAPGPVEKPPREPARQLRNPPTTIGMMTLKAAFKTLSGAQDSEAAFAKLDQKDLVLPTQALPASPALKNKRPRKDENESSAPADGEGGSELQPKNKRMTISRLVLEEDSQSTEPSAGLNSSQEAASAPPSKPTVLNQPLPGEKNPKVPKGKWNSSNGVEEKETWVEEDELFQVQAAPDEDSTTNITKKQKWTVEESEWVKAGVQKYGEGNWAAISKNYPFVNRTAVMIKDRWRTMKRLGMN blm-terf1-7 In vitro Co-IP: bait= TRF1 (TERF1) , prey = BLM 251834 N/A 251841 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 251842 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false blm-terf1-8 In vitro Co-IP: bait= TRF1 (TERF1) , prey = BLM helicase fragment 251834 N/A 251841 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false helicase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 251842 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false blm-terf2-9 In vitro Co-IP: bait= TRF2 (TERF2) , prey = BLM helicase fragment 251834 N/A 251841 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false helicase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 251843 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false blm-terf2-10 In vitro Co-IP: bait= TRF2 (TERF2) , prey = BLM c-terminus fragment 251834 N/A 251841 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 251843 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false blm-terf2-1 In vivo Co-IP: bait= BLM, prey =TRF2 251835 N/A 251841 unspecified role unspecified role bait bait N/A 251843 unspecified role unspecified role prey prey physical association physical association false false false blm-terf2-2 In vivo Co-IP: bait= BLM, prey =TRF2 251836 N/A 251841 unspecified role unspecified role bait bait N/A 251843 unspecified role unspecified role prey prey physical association physical association false false false blm-terf2-5 Colocalization of BLM and TRF2 (TERF2) in VA13/2RA cells 251837 N/A 251841 unspecified role unspecified role neutral component neutral component N/A 251843 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false BLM and TRF2 co-localize with foci of DNA synthesis Co-localization is enriched in late S amd G2/M phases. BLM and TRF2 co-localize in telomerase-negative immortalized cells using ALT (Saos2, VA13/2R) but does not colocalize in telomerase-positive cells (MCF7) and primary cells (WI-38). blm-terf2-4 Colocalization of BLM and TRF2 (TERF2) in Saos2 cells 251838 N/A 251841 unspecified role unspecified role neutral component neutral component N/A 251843 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false BLM and TRF2 co-localize with foci of DNA synthesis Co-localization is enriched in late S amd G2/M phases. BLM and TRF2 co-localize in telomerase-negative immortalized cells using ALT (Saos2, VA13/2R) but does not colocalize in telomerase-positive cells (MCF7) and primary cells (WI-38). blm-terf1-6 ELISA: bait= BLM, prey = TRF1 (TERF1) 251839 N/A 251841 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 251842 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction false false false blm-terf2-6 In vitro Co-IP: bait= TRF2 (TERF2), prey = BLM 251840 N/A 251841 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 251843 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false false false