UniProt UniProt http://www.uniprot.org maytal_kivi-2002-1 COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Maytal-Kivity V., Piran R., Pick E., Hofmann K., Glickman MH. 1A glickman@tx.technion.ac.il EMBO Rep. (1469-221X) 2002 maytal_kivi-2002-2 COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast cosedimentation cosedimentation polyclonal western polyclonal antibody western blot polyclonal western Maytal-Kivity V., Piran R., Pick E., Hofmann K., Glickman MH. 1A glickman@tx.technion.ac.il EMBO Rep. (1469-221X) 2002 csn12_yeast Cop9 signalosome complex subunit 12 J1860 YJR084W CSN12 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDVDIGCYFEEKRYDDKLLDFIRYDVKTPKKTKYILQRPTATDEESVRLQRFYQLGVDLKLKYSKRRSLKKQGRIKNATEELLRLANEQLKLFNRIVERETNWIIYPLWVMAKQLIRLANESSELNKDSIEECGRTIHRSFTICLNDRNPRLNENKKIGCYMFANLEFSIYHRLSNKDMIKNLVKVLESRVNARDIPPLNKSLAMEHKSQVVLYNYYLGQYYGCLENDHERGFFHLNEALLQCPMLYVESTGKFVLQGQMEKIMILLVPLALLTKRLYPHWDHPVIAGVITRSKRLSQVYPTLVRSVISGNLSLYEATAASHERFFLSQGLHVVITLLREVVFTRLVQRCWQWGNDRKSIMPLKILLATKQHDSSANEDEEEQLDALECRLASAIASGLLRAYLSHSNRCIVFSKKEPFPHSK csi1_yeast Cop9 signalosome-interactor 1 YM9711.15 YMR025W CSI1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDLLKFSSLAISEINFLHESSFDSIDHSWFLLIGCKLDQDDEIYIPINGNEAESQWYIEKVIRIPMQENDKINQERLERRINLTKVTQKDICILGILDLCQLEEDENITNKVTEKVLTQLTALALKYLIKYNVFRQHTSFQEAVNSLKGYKIENSVQIGAEIILDFLQDKVQIKDVNDRYQIPTPNNTVDPGFDEFQLIDMKDKEINIQKYNNNTIRKLLEKINRMIIFLKNYDATDKPFSSTQDVILRKISMLVTQLQRGGTSDMNYLLDNKINEIKLLEISCKQWEISNMLKK csn9_yeast COP9 signalosome complex subunit 9 CSN9 YDR179C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVMREETIKSLEDPYKYHYKEEWLNTKDPDEQQLFEIFAFGNIKDLPENIILTSLMRSKLEKLTLVTLSEIYNELSYELIKEECQIEDDGIIESHLIQLQNIFKAEMDSVSKSMKFSRRFDCRDVYCHEKELTIIKNPRVTKEYLVQNLRSWETKLKQNILE csn10_yeast COP9 signalosome complex subunit 10 RRI2 CSN10 YOL117W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDEDNNYDDFMLSDDEGMESIEMEEETDDEDKQNIEINEDNSQDDQDRGAARHKQHEQGTFEKHDRVEDICERIFEQGQALKEDERYKEARDLFLKIYYKEEFSSDESIERLMTWKFKSLIEILRLRALQLYFQKNGAQDLVLQILEDTATMSVFLQRIDFQIDGNIFELLSDTFEVLAPKWERVFLFDIEKVDRENMICKIDFQKNFMDQFQWILRKPGKDCKLQNLQRIIRKKIFIAVVWYQRLTMGNVFTPEISSQIEILVKDNECSSFEENNDLESVSMLLQYYILEYMNTARINNRRLFKKCIDFFEMLISKSLTFSQESGLMVILYTSKIVFILDSDSENDLSFALMRYYDRKEELKNMFLYILKHLEEMGKLRERDITSLFHKFILSGFIFTSMILEAISTDKINPFGFEQVKIALGSPIVNVLEDVYRCFAQLELRQLNASISLIPELSVVLSGIIQDIYYLAQTLKLWRKIARLYSCISISDIISMLQISDDNEMTRDDLLTILMRSIMKNRSVVYFKLDLTSDLVYFGDENKVMLPRCSKEEFRLMISPKDEETTEKARLIDFEYVNDVAIYNNPTRIRTKSSKEFFNTLRKSRETVKLPRVSNQSNEDTFLPSYMKFSNKYLELCKLASNNLE csn5_yeast COP9 signalosome complex subunit 5 RRI1 CSN5 JAB1 D0888 YDL216C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLSNKTVKELRQLLKERYTVEDELTESIALSSMRFKPSQEPEFHALSQSSLLKTKLKQQSSTDIPSYTHVLISKLSCEKITHYAVRGGNIEIMGILMGFTLKDNIVVMDCFNLPVVGTETRVNAQLESYEYMVQYIDEMYNHNDGGDGRDYKGAKLNVVGWFHSHPGYDCWLSNIDIQTQDLNQRFQDPYVAIVVDPLKSLEDKILRMGAFRTIESKSDDNSATSYYELETIIFDSELNRALFETKLNLHCVIEDDESEQISLNRLIDSMKQYSYLMDSKNVRTRIKLATTSERVSNENKKNIDYQNRSTRSQFCLNTQRGDSTETSSFGSMFSGDNTSDVDMEDRNLTEFDSTDTSLCINGEPSIHVNRVERSSRSTDNFHNSKKRMNSNQERCHDEGNDMLQRNVLETDYARAKNRILASKIKQYERLRFYKDTFTL 56714AD214C32D36 csi1-csn12 Interaction detected by yeast two hybrid 232802 N/A 232804 unspecified role unspecified role prey prey N/A 232805 unspecified role unspecified role bait bait physical association physical association false false false csn9-csn12-1 Interaction detected by yeast two hybrid 232802 N/A 232804 unspecified role unspecified role prey prey N/A 232806 unspecified role unspecified role bait bait physical association physical association false false false csn9-csi1-1 Interaction detected by yeast two hybrid 232802 N/A 232805 unspecified role unspecified role prey prey N/A 232806 unspecified role unspecified role bait bait physical association physical association false false false csn10-csn12-1 Interaction detected by yeast two hybrid 232802 N/A 232807 unspecified role unspecified role bait bait N/A 232804 unspecified role unspecified role prey prey physical association physical association false false false csn12-csn12-1 Interaction detected by yeast two hybrid 232802 N/A 232804 unspecified role unspecified role prey prey N/A 232804 unspecified role unspecified role bait bait physical association physical association false false false csn5-csn12-1 Interaction detected by yeast two hybrid 232802 N/A 232804 unspecified role unspecified role prey prey N/A 232808 unspecified role unspecified role bait bait physical association physical association false false false csn5-csi1-1 Interaction detected by cosedimentation 232803 N/A 232808 unspecified role unspecified role neutral component neutral component c-ter C-terminal MYC tagged protein csn5 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false N/A 232805 unspecified role unspecified role neutral component neutral component c-ter C-terminal GST tagged protein Csi1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false This interaction requires the presence of Csn12p.