MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint feller-2002-1 SH3 domain-mediated binding of the Drk protein to Dos is an important step in signaling of Drosophila receptor tyrosine kinases. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Feller SM., Wecklein H., Lewitzky M., Kibler E., Raabe T. Mech. Dev. (0925-4773) 2002 feller-2002-2 SH3 domain-mediated binding of the Drk protein to Dos is an important step in signaling of Drosophila receptor tyrosine kinases. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Feller SM., Wecklein H., Lewitzky M., Kibler E., Raabe T. Mech. Dev. (0925-4773) 2002 dos_drome Protein daughter of sevenless dos CG1044 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDRTFYEGWLIKSPPTKRIWRARWRRRYFTLKQGEIPEQFCLEYYTDHNCRKLKGVIDLDQCEQVDCGLRLENRKQKFQYMFDIKTPKRTYYLAAETEADMRDWVNCICQVCHLHDTKQSNELPLGAVGADENRTQHTSSSGGLSNSTQNTTTTSLHSSAGTTAPQASVPNAGGSAQLRRPAVIEEQPMPSNAGNNNSDSVYVNTEYSNRETMLCDANFDQQELLSAAQQQPPPSPATALYLNHSALIQAQAAAAAAEQLQQQQQQAARLAVSANGVVRKLPEHLVLTQQTLAEAAAQQHSSVQASPALSTASGPYIPISECFSGSPRFLPGVPLPGADLAIPNNPTTPLNNLDPKFYDTPRSHNNIGLNLTNDQSYSPKITNLSLQQLANNNASKQRSDSDSESVFTDDDEWAHPLPLRENVDRSTRPSDSSIENESFVLTYSQRFSKMPEEGGAIVPPAEKSSKLAGAASLPEAGDQGTLDKLAKVLKNKNNLILDFKENEKIPRDLPQLSDTENTSPAIVARRNAHSAFIEESYDIPRSHQQPYYNVNQLLGERPVTSPHNSNPIAASTPNLMAADLGAVAAISAAANPGLMGEAQAVASSPTSARTLPRHCYTNAAPTKMEGNVFRYDFMEQADCPPVNRKLKPKVAGGLPVVEDKPPEEFPAKPPVGVDQLTNKLGAAQLQQPIGPPSVDRKCKPNAYKLGNSATMSPATRRSSGAPLSMVLPHETDVHSPAAANAFFHETRTLPRQQHRHHPNSPGSMSVQHQRTASAAAAMMSLTAAAAPKQQAAAQTEHKLQYFDLDVTNKPPLLNRSSMSVGNLYSQGGNGASGMRFAGVEAGGARAPVPSSVVYRSVDFVKTEAFKRIREERESSGNK grap_drome Growth factor receptor-bound protein 2 drk E(sev)2B Downstream of receptor kinase Grb2 Protein enhancer of sevenless 2B CG6033 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEAIAKHDFSATADDELSFRKTQILKILNMEDDSNWYRAELDGKEGLIPSNYIEMKNHDWYYGRITRADAEKLLSNKHEGAFLIRISESSPGDFSLSVKCPDGVQHFKVLRDAQSKFFLWVVKFNSLNELVEYHRTASVSRSQDVKLRDMIPEEMLVQALYDFVPQESGELDFRRGDVITVTDRSDENWWNGEIGNRKGIFPATYVTPYHS drk-dos 188748 dos_drome 188750 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 drk_drome 188751 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false F1 homomint mint drk-dos-1 188748 dos_drome 188750 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 drk_drome 188751 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F2 homomint mint drk-dos-2 188748 dos_drome 188750 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed binding site binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false proline rich tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 drk_drome 188751 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F2 homomint mint drk-dos-3 188748 dos_drome 188750 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false proline rich Stoichiometry: 1.0 drk_drome 188751 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F2 homomint mint drk-dos-4 188749 drk_drome 188751 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 dos_drome 188750 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false F3 homomint mint