UniProt UniProt http://www.uniprot.org nice-2002-1 Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the pre-autophagosomal structure is required for selective autophagy. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom monoclonal western monoclonal antibody western blot monoclonal western Nice DC., Sato TK., Stromhaug PE., Emr SD., Klionsky DJ. J. Biol. Chem. (0021-9258) 2002 snx4_yeast Sorting nexin-4 SNX4 YJL036W J1244 ATG24 CVT13 Autophagy-related protein 24 Cytoplasm to vacuole targeting protein 13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDKGKNDLTSKAKDKARGNPEKPPYWFEIIVSDPQKRTGDPGSSSGYVSYQISTKTNNTSFYDNRGDPESIIVVHRRYSDLLLLHDILLNRFPTCIIPPLPDKKVFQYIAGDRFSQRFTQKRCHSLQNFLRRVSLHPDLSQSKVFKTFLVSKDWESHRKVLQDSLQPNKDEVTDAFMNAFKTVHKQNEEFTEIREKSDKLDRTVTKIDKLFHKVVKKNDSMSEDYTKLGSNLQELQELVTGENEELAAKLKIFNEGVTQLSYGLQDLTKYLDYEYIVDLKDLEHYIDSMRQLIKLKDQKQIDYEELSDYLTRSIKEKNNLISGYGGSNFFANKLEELAGINQEASRREKINKLEGKITSLTGELENAKKVADGFEQECLKEIDHFESVKTAEIKKSLGSLADHHIEFYERILEAWEKVDDSL 10964322A1A22F16 atg20_yeast Autophagy-related protein 20 ATG20 YDL113C Cytoplasm to vacuole targeting protein 20 Sorting nexin-42 CVT20 SNX42 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLNDVQENAKLNSETRNTGKAEPPHGTTEYVAEAEISKNGVGSPKKSPKKGKVGKGDNNKVETELVHTALLEKDNPFMEEGPTGFTKSALLEIPGMRSHNLKNPNEDYEDDSEGLLPLNQESNAETCRTSLSGSINSMNGETSASEEPSVSNRKKSARIHILEAKRVSEGQGRAYIAYVIQFENSTVQRRYSDFESLRSILIRLFPMTLIPPIPEKQSIKNYGKSITGSSSKYLLPSEGSGSVDLSLSVIHASVNNSDEKLIRHRIRMLTEFLNKLLTNEEITKTSIITDFLDPNNHNWHEFVNSSSTFSSLPKSILQCNPLDPTNTTRIHAMLPIPGSSSQLLLNKESNDKKMDKERSKSFTNIEQDYKQYENLLDNGIYKYNRRTTKTYHDLKSDYNEIGEVFAQFAHEQAQVGELAEQLSYLSNAFSGSSISLEKLVGRLYYNINEPLNESVHMATSARELIKYRKLKYLQNEMIKKSLNSKRAQLEKLEAQNNEYKDVDKIIDNEMSKSHTINLERPNNNTGSGGKSYGGKLFNGFNKLASMVKDSVKYQETDPHTASINLKKEIEQLSESLEVTENDLEVISKVIKNDQLPKFSKEREVDLSEILKHYSRYMRNYARQNLEIWKEVKRHQDFA 160977A8BECB6D93 atg17_yeast Autophagy-related protein 17 ATG17 APG17 YLR423C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNEADVTKFVNNARKTLTDAQLLCSSANLRIVDIKKKLSSWQLSISKLNFLIVGLRQQGKFLYTILKEGIGTKLIQKQWNQAVLVVLVDEMKYWQYEITSKVQRLDGIVNELSISEKDDTDPSKLGDYISRDNVNLLNDKLKEVPVIERQIENIKLQYENMVRKVNKELIDTKLTDVTQKFQSKFGIDNLMETNVAEQFSRELTDLEKDLAEIMNSLTQHFDKTLLLQDKKIDNDEREELFKVVQGDDKELYNIFKTLHEVIDDVDKTILNLGQFLQAKIKEKTELHSEVSEIINDFNRNLEYLLIFKDISNLIDSFKNSCTQDIQTTKELCEFYDNFEESYGNLVLEAKRRKDVANRMKTILKDCEKQLQNLDAQDQEERQNFIAENGTYLPETIWPGKIDDFSSLYTLNYNVKNP 71504B6C76549B75 cvt13-cvt13 Interaction detected by affinity chromatography 172135 N/A 172136 unspecified role unspecified role bait bait prot-a-fusion Protein A-Cvt13 fusion protein tag tag certain certain sequence position certain certain certain sequence position certain false N/A 172136 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged Cvt13 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Cvt13 is part of a complex containing several Cvt13 units, Cvt20 and Apg17. cvt13-cvt20 Interaction detected by affinity chromatography 172135 N/A 172137 unspecified role unspecified role bait bait prot-a-fusion Protein A-Cvt20 fusion protein tag tag certain certain sequence position certain certain certain sequence position certain false N/A 172136 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged Cvt13 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Cvt13 is part of a complex containing several Cvt13 units, Cvt20 and Apg17. cvt13-apg17 Interaction detected by affinity chromatography 172135 N/A 172136 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged Cvt13 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag polyclonal western polyclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false N/A 172138 unspecified role unspecified role bait bait prot-a-fusion Protein A-Apg17 fusion protein tag tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Cvt13 is part of a complex containing several Cvt13 units, Cvt20 and Apg17.