IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gu-2002-5 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-6 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-3 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-7 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-4 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-1 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 gu-2002-2 Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Gu JY., Park JM., Song EJ., Mizuguchi G., Yoon JH., Kim-Ha J., Lee KJ., Kim YJ. Nuclear fractions used. J. Biol. Chem. (0021-9258) 2002 arm_drome Armadillo segment polarity protein arm CG11579 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSYMPAQNRTMSHNNQYNPPDLPPMVSAKEQTLMWQQNSYLGDSGIHSGAVTQVPSLSGKEDEEMEGDPLMFDLDTGFPQNFTQDQVDDMNQQLSQTRSQRVRAAMFPETLEEGIEIPSTQFDPQQPTAVQRLSEPSQMLKHAVVNLINYQDDAELATRAIPELIKLLNDEDQVVVSQAAMMVHQLSKKEASRHAIMNSPQMVAALVRAISNSNDLESTKAAVGTLHNLSHHRQGLLAIFKSGGIPALVKLLSSPVESVLFYAITTLHNLLLHQDGSKMAVRLAGGLQKMVTLLQRNNVKFLAIVTDCLQILAYGNQESKLIILASGGPNELVRIMRSYDYEKLLWTTSRVLKVLSVCSSNKPAIVDAGGMQALAMHLGNMSPRLVQNCLWTLRNLSDAATKVEGLEALLQSLVQVLGSTDVNVVTCAAGILSNLTCNNQRNKATVCQVGGVDALVRTIINAGDREEITEPAVCALRHLTSRHVDSELAQNAVRLNYGLSVIVKLLHPPSRWPLIKAVIGLIRNLALCPANHAPLREHGAIHHLVRLLMRAFQDTERQRSSIATTGSQQPSAYADGVRMEEIVEGTVGALHILARESHNRALIRQQSVIPIFVRLLFNEIENIQRVAAGVLCELAADKEGAEIIEQEGATGPLTDLLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSIELTNSLLREDNNIWANADLGMGPDLQDMLGPEEAYEGLYGQGPPSVHSSHGGRAFHQQGYDTLPIDSMQGLEISSPVGGGGAGGAPGNGGAVGGASGGGGNIGAIPPSGAPTSPYSMDMDVGEIDAGALNFDLDAMPTPPNDNNNLAAWYDTDC med17_drome Mediator of RNA polymerase II transcription subunit 17 MED17 CG7957 Mediator complex subunit 17 dMED17 dTRAP80 Trap80 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSNSVNISVETTCENQIREIGYDGTELYQPPPTLSESLAKCAARIDFSKTSLDDLKKEEKSAAAAADEDKDATQFQESLWPWDAVRNKLKDALTEICVLSDVISIAKDKRYLVLDPLLEEADDTKPIVQVYSRKKAISQAAQVLLSGAERLRNAHSEQRNRNVSDFHIELLRLRQNWRLKKVSNAIIGDLSYRTAGSKFGMSGTFEVTKAEETGDEDTASSSNSSSSVSGNNGMQLKASSALRVIVPAELQGVAYIKVITQKDQEDLCTAQLNLMGHGPNITAQVGVWQKTLEFAQNVLFCKELFAQLAREAIQLQAPIPHVVIGNQIRATLLPNIQLIISLCHSTTFDSSQPAPINDHDHVLEHSLHQLLREVHYKNSHHPFPHPASAPLGPTKKRMLAGPMAADRETLLDMTKSQTILEQIIAQAQHIFMRKRTQYVLDTLARDVKDPQIVSHWNAMNSPTMSCVKINIVTHGYDAIGRTSLVIHVKERSLKCICRDGRVMRLSYEPQELRDLILCQINSHQISCLISLARCMSWTVLSNSNHLGIGKVEPLGNASSCLLASPNSDRMIAVQIRCDPQIDVKVYIARSPRQDFFPSPLVPEKLWENLGGTFKEVRFDKIEGKSFLNKMEFLMASLTSNTA dif_drome Dorsal-related immunity factor Dif Dif CG6794 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFEEAFGDIQEIINASMELNGGATGGGSVAGAVGGGGAAHHILSQSTSLPVMPSHIPLHLQNQNMNQNLPEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGKTFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVGIQCAKKLEMRDSLVERERRNIDPFNAKFDHKDQIDKINRYELRLCYQAFITVGNSKVPLDPIVSSPIYGKSSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPELLTKHNRRVAQKTVESLKRSLMSTNLHPSKQVKTSSQYTIFSKPQIATTTPQTQVSPGMPLMFPGGSPNFVQDIKMENGFMDVDSQSSQCPSVERNFASPRSNCSTVDSIPPMQMGQNQTHLYLPDATNFTFNGNFASPSSNCSTVDSIPPFQIGQRNNHMYLPENSNFPVNGCSPTHFSGGSMTPINNNNNVLINNNNNDFLSQKMSAISIPPQGNFGIKQVYQQTQQFLPQLQPESIPYLAQSHPEQSQYQQQQQPQEQQPPADEPTQSFSDLISSSIGMAPIDTSELIQDIEAELNSLGIQPFK hsf_drome Heat shock factor protein Hsf CG5748 Heat shock transcription factor protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL notch_drome Neurogenic locus Notch protein N CG3936 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSQRSRRRSRAPNTWICFWINKMHAVASLPASLPLLLLTLAFANLPNTVRGTDTALVAASCTSVGCQNGGTCVTQLNGKTYCACDSHYVGDYCEHRNPCNSMRCQNGGTCQVTFRNGRPGISCKCPLGFDESLCEIAVPNACDHVTCLNGGTCQLKTLEEYTCACANGYTGERCETKNLCASSPCRNGATCTALAGSSSFTCSCPPGFTGDTCSYDIEECQSNPCKYGGTCVNTHGSYQCMCPTGYTGKDCDTKYKPCSPSPCQNGGICRSNGLSYECKCPKGFEGKNCEQNYDDCLGHLCQNGGTCIDGISDYTCRCPPNFTGRFCQDDVDECAQRDHPVCQNGATCTNTHGSYSCICVNGWAGLDCSNNTDDCKQAACFYGATCIDGVGSFYCQCTKGKTGLLCHLDDACTSNPCHADAICDTSPINGSYACSCATGYKGVDCSEDIDECDQGSPCEHNGICVNTPGSYRCNCSQGFTGPRCETNINECESHPCQNEGSCLDDPGTFRCVCMPGFTGTQCEIDIDECQSNPCLNDGTCHDKINGFKCSCALGFTGARCQINIDDCQSQPCRNRGICHDSIAGYSCECPPGYTGTSCEININDCDSNPCHRGKCIDDVNSFKCLCDPGYTGYICQKQINECESNPCQFDGHCQDRVGSYYCQCQAGTSGKNCEVNVNECHSNPCNNGATCIDGINSYKCQCVPGFTGQHCEKNVDECISSPCANNGVCIDQVNGYKCECPRGFYDAHCLSDVDECASNPCVNEGRCEDGINEFICHCPPGYTGKRCELDIDECSSNPCQHGGTCYDKLNAFSCQCMPGYTGQKCETNIDDCVTNPCGNGGTCIDKVNGYKCVCKVPFTGRDCESKMDPCASNRCKNEAKCTPSSNFLDFSCTCKLGYTGRYCDEDIDECSLSSPCRNGASCLNVPGSYRCLCTKGYEGRDCAINTDDCASFPCQNGGTCLDGIGDYSCLCVDGFDGKHCETDINECLSQPCQNGATCSQYVNSYTCTCPLGFSGINCQTNDEDCTESSCLNGGSCIDGINGYNCSCLAGYSGANCQYKLNKCDSNPCLNGATCHEQNNEYTCHCPSGFTGKQCSEYVDWCGQSPCENGATCSQMKHQFSCKCSAGWTGKLCDVQTISCQDAADRKGLSLRQLCNNGTCKDYGNSHVCYCSQGYAGSYCQKEIDECQSQPCQNGGTCRDLIGAYECQCRQGFQGQNCELNIDDCAPNPCQNGGTCHDRVMNFSCSCPPGTMGIICEINKDDCKPGACHNNGSCIDRVGGFECVCQPGFVGARCEGDINECLSNPCSNAGTLDCVQLVNNYHCNCRPGHMGRHCEHKVDFCAQSPCQNGGNCNIRQSGHHCICNNGFYGKNCELSGQDCDSNPCRVGNCVVADEGFGYRCECPRGTLGEHCEIDTLDECSPNPCAQGAACEDLLGDYECLCPSKWKGKRCDIYDANYPGWNGGSGSGNDRYAADLEQQRAMCDKRGCTEKQGNGICDSDCNTYACNFDGNDCSLGINPWANCTANECWNKFKNGKCNEECNNAACHYDGHDCERKLKSCDSLFDAYCQKHYGDGFCDYGCNNAECSWDGLDCENKTQSPVLAEGAMSVVMLMNVEAFREIQAQFLRNMSHMLRTTVRLKKDALGHDIIINWKDNVRVPEIEDTDFARKNKILYTQQVHQTGIQIYLEIDNRKCTECFTHAVEAAEFLAATAAKHQLRNDFQIHSVRGIKNPGDEDNGEPPANVKYVITGIILVIIALAFFGMVLSTQRKRAHGVTWFPEGFRAPAAVMSRRRRDPHGQEMRNLNKQVAMQSQGVGQPGAHWSDDESDMPLPKRQRSDPVSGVGLGNNGGYASDHTMVSEYEEADQRVWSQAHLDVVDVRAIMTPPAHQDGGKHDVDARGPCGLTPLMIAAVRGGGLDTGEDIENNEDSTAQVISDLLAQGAELNATMDKTGETSLHLAARFARADAAKRLLDAGADANCQDNTGRTPLHAAVAADAMGVFQILLRNRATNLNARMHDGTTPLILAARLAIEGMVEDLITADADINAADNSGKTALHWAAAVNNTEAVNILLMHHANRDAQDDKDETPLFLAAREGSYEACKALLDNFANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMLSMTPQAMIGSPPPGQQQPQLITQPTVISAGNGGNNGNGNASGKQSNQTAKQKAAKKAKLIEGSPDNGLDATGSLRRKASSKKTSAASKKAANLNGLNPGQLTGGVSGVPGVPPTNSAAQAAAAAAAAVAAMSHELEGSPVGVGMGGNLPSPYDTSSMYSNAMAAPLANGNPNTGAKQPPSYEDCIKNAQSMQSLQGNGLDMIKLDNYAYSMGSPFQQELLNGQGLGMNGNGQRNGVGPGVLPGGLCGMGGLSGAGNGNSHEQGLSPPYSNQSPPHSVQSSLALSPHAYLGSPSPAKSRPSLPTSPTHIQAMRHATQQKQFGGSNLNSLLGGANGGGVVGGGGGGGGGVGQGPQNSPVSLGIISPTGSDMGIMLAPPQSSKNSAIMQTISPQQQQQQQQQQQQQHQQQQQQQQQQQQQQQQQLGGLEFGSAGLDLNGFCGSPDSFHSGQMNPPSIQSSMSGSSPSTNMLSPSSQHNQQAFYQYLTPSSQHSGGHTPQHLVQTLDSYPTPSPESPGHWSSSSPRSNSDWSEGVQSPAANNLYISGGHQANKGSEAIYI med6_drome Mediator of RNA polymerase II transcription subunit 6 MED6 CG9473 Mediator complex subunit 6 dMED6 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASRQMTNDHLRLSWHDTQMMATLSPQTVMDYFCRKSNPFYDHMCNNETVRMQRLGPEHLHNMIGLEYILLHVAEPILYVIRKQHRHNPSEATPIADYYIIGGTVYKAPDLANVINSRILNTVVNLQSAFEEASSYARYHPNKGYTWDFSSNKVFSDRSKSDKKDANSAKDENSGTLFQKQRVDMLLAELLRKFPPPIPPMLQNLQQPPPAGDDLNTARNASEMNNATGPLDIKTEGVDMKPPPEKKSK med30_drome Mediator of RNA polymerase II transcription subunit 30 MED30 CG17183 dMED20 dTRAP25 Trap25 Mediator complex subunit 30 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MWKYGQNQGNQGPSSGGGGGGGPNMMPMGGFGMQHGNMQQMHMSPQHQQQQQQMGMMGGPGSMQMNPQGPGGPGGLMPGMSPQHQMQQQQQQQMMQQQMMVPQQGVGVGVGMGGGVGMGGGGVVPQQQQQQPQQNMPQQNIPQQQQQLNPVAGIPPGGAGGSNNMLAISQQNPHKEINIVQLSRLGQETVQDIASRFQEVFASLKGIQPTSHRENSSEKKVQEYFRTIRLLFKRVRIIYEKCNDAGMDYMSAESLIPYRDEPEPRIEPSLCDEYRKVLQENHELIETVKLKNRQLREIIDRTRIIIWEINTMLAMRRS med22_drome Mediator of RNA polymerase II transcription subunit 22 MED22 dMED24 Med24 Mediator complex subunit 22 CG3034 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASGSRTTILPQSKEALLKSYNARLKDDVRSMLENFEEILKLARRESHSQISKTTQCEQDALEMQVRAANMVRAGESLMKLVADLKQYLILNDFHSVNEAITNNSQLFRNTQSECDKKLMKLRDEMAMDLYDLEEEYYTSIFK med11_drome Mediator of RNA polymerase II transcription subunit 11 MED11 CG6884 Med21 Mediator complex subunit 11 dMED21 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNPLDKIHALDEIEKEIILCMQSAGQALQELGKEKSSQKNAETQSQQFLKSLSSVESKLSEQINYLTQVSTGQPHEGSGYASAKVLQMAWHRIQHARSRVRELEETKAKHSHAARQQLKRQQEHAAAQQQQQQQQQQQQQQQQMQQAAQQQQQQTGGGNAGSGDHPMGGDSSMSTN med28_drome Mediator of RNA polymerase II transcription subunit 28 MED28 CG5121 Mediator complex subunit 28 dMED23 Med23 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASNESGGGNLMDEFEEAFQSCLLTLTKQEPNSGTNKEEIDLEVQKTTNRFIDVARQMEAFFLQKRFLVSTLKPYMLIKDENQDLSIEIQRKEALLQKHYNRLEEWKACLSDIQQGVHSRPTPPIGSGMLQGPGGGMPPMGGTPPRPGMMPGMPPGAMQPGGPMQPSPHMLQAQQMQQLRMISRQMPPK med20_drome Mediator of RNA polymerase II transcription subunit 20 MED20 Tmr CG18780 Mediator complex subunit 20 dMED20 Protein trf-proximal dTrfp dTrap26 Trfp protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGVTILQPYPLPEGKSGAHIIDQLSKRLLALGATHAGQFLVDCETFISTPQPHNGAPGRAVHVLHNSEYPASTFSIIDNGTGKQVAIVADNIFDLLMLKMTNTFTSKKQTKIESRGARFEYGDFVIKLGSVTMMEHFKGILIEIEYKSCVILAYCWEMIREMLQGFLGIAVNKDFPSYFAPQTIMTAMGQQQLHAKHNDIFEPMDTVKQYLEQFTNYRKHVTLMGGMGSGPGSQQVGPNVHMSPAVAGLHRS med21_drome Mediator of RNA polymerase II transcription subunit 21 Mediator complex subunit 21 dMED21 dTRAP19 dSRB7 Trap19 CG17397 MED21 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADRLTQLQDTVNQQAEHFCNAIGVIQQTSLPSKFVNFERIGPQTPIPCPPQEDYAQLFAQLIARCAKDIDTLIESLPNEDSSIELQNSSLKRLEIENQGTARDLEEVVQKGELLLEKMQYSLECIAQAQLDMQITLKHDLQ med4_drome Mediator of RNA polymerase II transcription subunit 4 MED4 CG8609 Trap36 Mediator complex subunit 4 dp34 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSFHLSTKERLLLLIDDIEMIAKELIEQAHQKISSTELVDLLDLLVAKDEEFRKMLELAEEQAKVEEAMDQLRAKVEVHDREIQKLQKSLKDAELILSTAIFQARQKLASINQANKRPVSSEELIKYAHRISSANAVSAPLTWCIGDLRRPYPTDIEMRNGLLGKSEQNINGGTVTHQNSGMPSEQQRTLSGSAGSGSGSGAGGEVPNAFQNQFNWNLGELHMTMGASGNTVALETRAQDDVEVMSTDSSSSSSSDSQ med31_drome Mediator of RNA polymerase II transcription subunit 31 MED31 CG1057 Mediator complex subunit 31 Protein SOH1 dSOH1 Trap18 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAKMYGKGKTAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN rpb9_drome DNA-directed RNA polymerase II subunit RPB9 RpII15 DNA-directed RNA polymerase II 15.1 kDa polypeptide CG3284 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTAFDAAHTEGPGFVGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE rpb1_drome DNA-directed RNA polymerase II subunit RPB1 RpII215 CG1554 DNA-directed RNA polymerase III largest subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTPTDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNTLGNSMLGGAAMFIGGGSTPSMTPPMTPWANCNTPRYFSPPGHVSAMTPGGPSFSPSAASDASGMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPGGASPNYSPSSPNYSPTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSSPQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPTFEESED arm-trap80 interaction detected by GST pull down. 179424 N/A 179431 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 179432 unspecified role unspecified role prey prey physical association physical association false false false trap80-dif interaction detected by GST pull down. 179424 N/A 179433 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 179432 unspecified role unspecified role prey prey physical association physical association false false false trap80-hsf interaction detected by GST pull down. 179424 N/A 179434 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 179432 unspecified role unspecified role prey prey physical association physical association false false false trap80-n interaction detected by GST pull down. 179424 N/A 179435 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 179432 unspecified role unspecified role prey prey physical association physical association false false false trap25-med6 179425 N/A 179432 unspecified role unspecified role prey prey N/A 179436 unspecified role unspecified role prey prey N/A 179437 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false med24-med6 179425 N/A 179436 unspecified role unspecified role prey prey N/A 179438 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 179432 unspecified role unspecified role prey prey association association false false false med21-med6 179425 N/A 179432 unspecified role unspecified role prey prey N/A 179439 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 179436 unspecified role unspecified role prey prey association association false false false med23-med6 179425 N/A 179432 unspecified role unspecified role prey prey N/A 179436 unspecified role unspecified role prey prey N/A 179440 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false trap80-med6-2 Complex purified by chromatography. 179426 N/A 179432 unspecified role unspecified role neutral component neutral component N/A 179441 unspecified role unspecified role neutral component neutral component N/A 179436 unspecified role unspecified role neutral component neutral component physical association physical association false false false C1 complex trap80-med6-3 Complex purified by chromatography. 179426 N/A 179442 unspecified role unspecified role neutral component neutral component N/A 179443 unspecified role unspecified role neutral component neutral component N/A 179441 unspecified role unspecified role neutral component neutral component N/A 179444 unspecified role unspecified role neutral component neutral component N/A 179432 unspecified role unspecified role neutral component neutral component N/A 179436 unspecified role unspecified role neutral component neutral component physical association physical association false false false C2 mediator complex trap80-med6-4 Complex purified by chromatography. 179426 N/A 179441 unspecified role unspecified role neutral component neutral component N/A 179442 unspecified role unspecified role neutral component neutral component N/A 179443 unspecified role unspecified role neutral component neutral component N/A 179444 unspecified role unspecified role neutral component neutral component N/A 179432 unspecified role unspecified role neutral component neutral component N/A 179436 unspecified role unspecified role neutral component neutral component physical association physical association false false false C3 mediator complex This corresponds in elution profile to the dMediator complex observed in Drosophila embryos. med6-trfp 179427 N/A 179436 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 179441 unspecified role unspecified role prey prey s35 radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false med6-trap80 179427 N/A 179432 unspecified role unspecified role prey prey s35 radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 179436 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false med6-trfp-1 179427 N/A 179432 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 179441 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false rpii15-med6 Interaction detected by anti flag coIp 179428 N/A 179436 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. N/A 179445 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association false false false rpii15-rpii215 Interaction detected by anti flag coIp 179428 N/A 179445 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. N/A 179446 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association false false false trap80-med6 Interaction detected on a superose 6 column. 179429 N/A 179441 unspecified role unspecified role neutral component neutral component N/A 179436 unspecified role unspecified role neutral component neutral component N/A 179432 unspecified role unspecified role neutral component neutral component physical association physical association false false false trap80-med6-1 Interaction detected on a superose 6 column. 179430 N/A 179441 unspecified role unspecified role neutral component neutral component N/A 179436 unspecified role unspecified role neutral component neutral component N/A 179432 unspecified role unspecified role neutral component neutral component physical association physical association false false false