IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nishihama-2002-1 Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nishihama R., Soyano T., Ishikawa M., Araki S., Tanaka H., Asada T., Irie K., Ito M., Terada M., Banno H., Yamazaki Y., Machida Y. NACK1 and NPK1 were coimmunoprecipitation from BY-2 cells that had been harvested 1.5 hr after two-step synchronization. Figure 3 (D) yas@biol1.bio.nagoya-u.ac.jp Cell (0092-8674) 2002 nishihama-2002-4 Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nishihama R., Soyano T., Ishikawa M., Araki S., Tanaka H., Asada T., Irie K., Ito M., Terada M., Banno H., Yamazaki Y., Machida Y. NACK1 and NPK1 were coimmunoprecipitation from BY-2 cells that had been harvested 1.5 hr after two-step synchronization. Figure 3 (D) yas@biol1.bio.nagoya-u.ac.jp Cell (0092-8674) 2002 nishihama-2002-2 Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. tobac Nicotiana tabacum (Common tobacco) Common tobacco fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Nishihama R., Soyano T., Ishikawa M., Araki S., Tanaka H., Asada T., Irie K., Ito M., Terada M., Banno H., Yamazaki Y., Machida Y. NACK1 and NPK1 were coimmunoprecipitation from BY-2 cells that had been harvested 1.5 hr after two-step synchronization. Figure 3 (D) yas@biol1.bio.nagoya-u.ac.jp Cell (0092-8674) 2002 nishihama-2002-3 Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. An undifferentiated cell line derived from tobacco callus tissue. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nishihama R., Soyano T., Ishikawa M., Araki S., Tanaka H., Asada T., Irie K., Ito M., Terada M., Banno H., Yamazaki Y., Machida Y. NACK1 and NPK1 were coimmunoprecipitation from BY-2 cells that had been harvested 1.5 hr after two-step synchronization. Figure 3 (D) yas@biol1.bio.nagoya-u.ac.jp Cell (0092-8674) 2002 npk1_tobac Mitogen-activated protein kinase kinase kinase NPK1 NPK1 Nicotiana protein kinase 1 protein protein tobac Nicotiana tabacum (Common tobacco) Common tobacco MQDFIGSVRRSLVFKQSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPALPSISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKFGSFPESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFMGNPENMAAQRMDVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNSDDDMCQMDNDDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQANLHYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQTPLYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSPVTHSKRISNIGGLNGEAIQEAQLPRHNEWKDLLGSQREAVNSSFSERQRRWKEELDEELQRKREIMRQAVNLSPPKDPILNRCRSKSRFASPGR kn7a_tobac Kinesin-like protein NACK1 NPK1-activating kinesin-1 NACK1 protein protein tobac Nicotiana tabacum (Common tobacco) Common tobacco MTVRTPGTPASKIDKTPATTPNGHRGREEKIVVTVRLRPLNKRELSAKDHAAWECIDDHTIIYRPVPQERAAQPASSFTFDKVFGPDSITEAVYEEGVKNVALSSLMGINATIFAYGQTSSGKTYTMRGITEKAVNDIYAHIMSTPEREFRIRISGLEIYNENVRDLLNSESGRSLKLLDDPEKGTVVEKLVEETASNDQHLRHLISICEAQRQVGETALNDTSSRSHQIIRLTIESTLRESSDCVRSYVASLNFVDLAGSERASQTNADGARLREGCHINLSLMTLTTVIRKLSVGKRSGHIPYRDSKLTRILQHSLGGNARTAIICTLSPASSHVEQSRNTLYFATRAKEVTNNAQVNMVVSDKQLVKHLQKEVARLEAELRTPDPANEKDWKIQQMEMEIEELKRQRDLAQSQVDELRRKLQEEQGPKPSESVSPVVKKCLSFSGTLSPNLEEKAPVRSERTRNTMGRQSMRQSLAAPFTLMHEIRKLEHLQEQLGDEANRALEVLQKEVACHRLGNQDAAETIAKLQAEIREMRSIRPLPKEVEVGSVVAVNKSVSANLKEEIARLHSQGSTIADLEEQLENVQKSLDKLVMSLPSNNDQQSNNDTTQKAKHPSKKKKLLPLTSSNSINRQNFLKSPCSPLSTARQVLDCEVENRAPDSDDLSCEIQPDETPTKSDGGDVSSKEGTPYRRSSSVNMRKMQKMFQEAAEENVRNIRSYVTELKERVAKLQYQKQLLVCQVLELEANEAAGYNLEDDENIHQIPEESPVSWQITFKEQRQQIIDLWDVCYVSIIHRSQFYLLFKGDPADEIYLEVELRRLTWLQQHLAELGNATPARVGNEPTVSLSSSIRALKREREFLAKRLTTRLTAEERDYLYIKWEVPLEGKQRRMQFINKLWTNPHDAKHVHESAEIVAKLVGFCEGGNMSREMFELNFVLPSDRRPWFAGWNQISDLLHI npk1-nack1 Anti bait coimmunoprecipitaion reveals interaction between the NPK1 and NACK1 proteins 209884 N/A 209888 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 209889 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false nack1-npk1 Anti bait coimmunoprecipitaion reveals interaction between the NACK1 and NPK1 proteins 209884 N/A 209888 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 209889 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false nack1-npk1-2 Anti bait coimmunoprecipitaion reveals interaction between the NACK1 and NPK1 proteins 209885 N/A 209888 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 209889 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false npk1-nack1-2 Anti bait coimmunoprecipitaion reveals interaction between the NPK1 and NACK1 proteins 209885 N/A 209889 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 209888 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false npk1-nack1-1 Co-localisation analysis reveals interaction between the NPK1 and NACK1 proteins 209886 N/A 209889 unspecified role unspecified role neutral component neutral component N/A 209888 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false NPK1 appears to be recruited to the phragmoplast equator through the carboxy-terminal region, probably via association with NACK1. nack1-npk1-1 Anti bait coimmunoprecipitaion reveals interaction between the NACK1 and NPK1 proteins 209887 N/A 209889 unspecified role unspecified role bait bait tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. An undifferentiated cell line derived from tobacco callus tissue. N/A 209888 unspecified role unspecified role prey prey tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. An undifferentiated cell line derived from tobacco callus tissue. physical association physical association false false false These data revealed the physical interaction between NACK1 and NPK1 at late M phase in tobacco cells.