IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sakuma-2001-1 Cloning and expression pattern of a novel PEBP2 beta-binding protein (charged amino acid rich leucine zipper-1[Crl-1]) in the mouse. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Sakuma T., Li QL., Jin Y., Choi LB., Kim EG., Ito K., Ito Y., Nomura S., Bae SC. A mouse brain cDNA library was used in the detection of Crlz1. Two proteins involved: Crlz1 and Cbfb. Mech. Dev. (0925-4773) 2001 sas10_mouse Something about silencing protein 10 Crlz1 Disrupter of silencing SAS10 UTP3 homolog Charged amino acid-rich leucine zipper 1 Utp3 Sas10 Crl1 protein protein mouse Mus musculus Mouse MVKKSRRRGAAQWAAVRAQAGLTATDENEDDLGLPPSPGDSSYYQDQVDEFHEARSRAVLAKGWNEVESGEEDGDEEEEVLPLDIDDGDDEDGESSEEEEVGEDDDGGSSVQSEAEASVDPSLSWGQRKKLYYDTDYGSKSRGRQSQQEVEEEEREEEEEAQIIQRRLAQALQEDDFGVAWVEAFAKPVPQVDEAETRVVKDLAKVSVKEKLKMLKKESPELLELIEDLQAKLTEVKDELEPLLQLVEKGVIPTGRGSEYLKTKYNLYLNYCANISFYLILKARRVPAHGHPVIERLVTYRNLINKLSVVDQKLSSEIRHLLTAKDGAVKKEMTPKAKLTKTKPKSVKQAAAVALTDEPDFDGAALKYYKEMEDRQELKRKKEENSAEEQALEEQNAKRAITYQIAKNRGLTPRRKKIDRNPRVKHREKFRKAKIRRRGQVREVRREEQRYSGELSGIRAGVKKSIKLK pebb_mouse Core-binding factor subunit beta Polyomavirus enhancer-binding protein 2 beta subunit SL3-3 enhancer factor 1 subunit beta SL3/AKV core-binding factor beta subunit Cbfb Pebp2b Pebpb2 protein protein mouse Mus musculus Mouse MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDDLKLR crlz1-cbfb-1 Y2H 168615 N/A 168616 unspecified role unspecified role prey prey in vitro In vitro N/A 168617 unspecified role unspecified role bait bait crlz1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false The Y2H beta-galactosidase activity assay was used to determine the region of interaction between the three alternate splice variants of cbfb and crlz1. This showed that crlz1 interacts with a 17aa region unique only to the c-terminus of the second alternate splice variant of cbfb.