IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ skoudy-2000-1 CD44 binds to the Shigella IpaB protein and participates in bacterial invasion of epithelial cells. in vitro In vitro pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Skoudy A., Mounier J., Aruffo A., Ohayon H., Gounon P., Sansonetti P., Tran Van Nhieu G. Cell. Microbiol. (1462-5814) 2000 skoudy-2000-2 CD44 binds to the Shigella IpaB protein and participates in bacterial invasion of epithelial cells. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay monoclonal western monoclonal antibody western blot monoclonal western Skoudy A., Mounier J., Aruffo A., Ohayon H., Gounon P., Sansonetti P., Tran Van Nhieu G. Cell. Microbiol. (1462-5814) 2000 skoudy-2000-3 CD44 binds to the Shigella IpaB protein and participates in bacterial invasion of epithelial cells. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa monoclonal western monoclonal antibody western blot monoclonal western Skoudy A., Mounier J., Aruffo A., Ohayon H., Gounon P., Sansonetti P., Tran Van Nhieu G. Cell. Microbiol. (1462-5814) 2000 skoudy-2000-4 CD44 binds to the Shigella IpaB protein and participates in bacterial invasion of epithelial cells. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip monoclonal western monoclonal antibody western blot monoclonal western Skoudy A., Mounier J., Aruffo A., Ohayon H., Gounon P., Sansonetti P., Tran Van Nhieu G. This experiment is carried out with Shigella flexneri strain M90T serotype 5. Negative controls are performed with the non-invasive derivate of M90T strain cured of the virulence plasmid carrying the IPA-B/C/D genes. Cell. Microbiol. (1462-5814) 2000 ipab_shifl Invasin IpaB 62 kDa antigen ipaB CP0128 protein protein shifl Shigella flexneri MHNVSTTTTGFPLAKILTSTELGDNTIQAANDAANKLFSLTIADLTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKITAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQIQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQANRTDVAKAILQQTTA cd44_human CD44 antigen Epican CDw44 Phagocytic glycoprotein I Extracellular matrix receptor III HUTCH-I Phagocytic glycoprotein 1 GP90 lymphocyte homing/adhesion receptor Hermes antigen Hyaluronate receptor Heparan sulfate proteoglycan CD44 LHR MDU2 MDU3 MIC4 protein protein human Homo sapiens Human MDKFWWHAAWGLCLVPLSLAQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPKEDSHSTTGTAAASAHTSHPMQGRTTPSPEDSSWTDFFNPISHPMGRGHQAGRRMDMDSSHSITLQPTANPNTGLVEDLDRTGPLSMTTQQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPHTKESRTFIPVTSAKTGSFGVTAVTVGDSNSNVNRSLSGDQDTFHPSGGSHTTHGSESDGHSHGSQEGGANTTSGPIRTPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINSGNGAVEDRKPSGLNGEASKSQEMVHLVNKESSETPDQFMTADETRNLQNVDMKIGV ipab-cd44-1 gst pull down of shigella ipaB and human receptor CD44 169758 N/A 169762 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 169763 unspecified role unspecified role prey prey biotin_extracell_dom hela cells are treated with biotin to tag all receptors tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Competitive inhibition of this interaction is observed when GST-complexes are incubated with anti-cd44 antibodies. ipab-cd44-2 filter binding of shigella ipaB and human receptor CD44 169759 N/A 169762 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 169763 unspecified role unspecified role prey prey ig cl domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false extracellular domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false IC50=150+/- 25nM ipab-cd44-3 ELISA of shigella ipaB and human receptor CD44 169760 N/A 169762 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 169763 unspecified role unspecified role prey prey ig cl domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false extracellular domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false The binding of bioin CD44-ig to IpaB-gst is inhibated by CD44-ig with a half maximum inhibitory concentration of (IC50=) 175+/- 20nM The binding of CD44-ig to IpaB-gst is saturable with a half maximal binding concentration of 150 +/- 25nM ipab-cd44-4 Coip of shigella ipaB and human receptor CD44 after infection of Hela cells 169761 N/A 169762 unspecified role unspecified role bait bait shifl Shigella flexneri N/A 169763 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Competitive inhibition of this interaction is observed when Hela cells are incubated with anti-cd44 antibodies during the shigella infection. Immunofluorescence show that this antibody treatment inhibit shigella internalisation. IpaB-CD44 interaction is required in the early steps of shigella invasion. The CoIp is carried out after shigella infection of the Hela cells.