IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mas-2000-1 Functional interaction of phytochrome B and cryptochrome 2. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mas P., Devlin PF., Panda S., Kay SA. Transgenic arabidopsis thaliana plants over-expressing cry2 were used in this study Nature (0028-0836) 2000 mas-2000-2 Functional interaction of phytochrome B and cryptochrome 2. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Mas P., Devlin PF., Panda S., Kay SA. Transgenic arabidopsis thaliana plants over-expressing cry2 were used in this study Nature (0028-0836) 2000 mas-2000-3 Functional interaction of phytochrome B and cryptochrome 2. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Mas P., Devlin PF., Panda S., Kay SA. Transgenic arabidopsis thaliana plants over-expressing cry2 were used in this study Nature (0028-0836) 2000 phyb_arath Phytochrome B PHYB HY3 OOP1 Protein LONG HYPOCOTYL 3 Protein OUT OF PHASE 1 MSF3.17 At2g18790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRMACPGEGLPPELVRDMFHSSRWTSPEGLGLSVCRKILKLMNGEVQYIRESERSYFLIILELPVPRKRPLSTASGSGDMMLMMPY cry2_arath Cryptochrome-2 Blue light photoreceptor CRY2 PHH1 Protein PHR homolog 1 Protein SUPPRESSOR OF elf3 20 SEL20 F19P19.14 At1g04400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYDPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEPGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVFFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK phyb-cry2 Coimmunoprecipitation reveals interaction between phytochrome B (phyB) and crypotochrome 2 (cry2) 178503 N/A 178506 unspecified role unspecified role bait bait N/A 178507 unspecified role unspecified role prey prey physical association physical association false false false phyb-cry2-1 Colocalisation analysis reveals interaction between phytochrome B (phyB) and crypotochrome 2 (cry2) 178504 N/A 178506 unspecified role unspecified role neutral component neutral component gfp green florescent protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 178507 unspecified role unspecified role neutral component neutral component rfp red florescent protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false The photoreceptors colocalised to the nucleus phyb-cry2-2 Colocalisation analysis reveals interaction between phytochrome B (phyB) and crypotochrome 2 (cry2) 178505 N/A 178507 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept rfp red florescent protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 178506 unspecified role unspecified role fluorescence donor fluorescence donor gfp green florescent protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false The photoreceptors colocalised to the nucleus