IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ burns-2000-3 Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Burns K., Clatworthy J., Martin L., Martinon F., Plumpton C., Maschera B., Lewis A., Ray K., Tschopp J., Volpe F. EL-4.6.10 cells were treated with 50ng/ml interleukin 1 over a time course of 0 to 10 minutes and coimmunoprecipiations carried out on cell isolates. Nat. Cell Biol. (1465-7392) 2000 burns-2000-2 Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Burns K., Clatworthy J., Martin L., Martinon F., Plumpton C., Maschera B., Lewis A., Ray K., Tschopp J., Volpe F. Nat. Cell Biol. (1465-7392) 2000 burns-2000-1 Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Burns K., Clatworthy J., Martin L., Martinon F., Plumpton C., Maschera B., Lewis A., Ray K., Tschopp J., Volpe F. Nat. Cell Biol. (1465-7392) 2000 il1r1_mouse Interleukin-1 receptor type 1 Il1r1 Il-1r1 Il1ra Interleukin-1 receptor type I p80 CD121 antigen-like family member A Interleukin-1 receptor alpha protein protein mouse Mus musculus Mouse MENMKVLLGLICLMVPLLSLEIDVCTEYPNQIVLFLSVNEIDIRKCPLTPNKMHGDTIIWYKNDSKTPISADRDSRIHQQNEHLWFVPAKVEDSGYYYCIVRNSTYCLKTKVTVTVLENDPGLCYSTQATFPQRLHIAGDGSLVCPYVSYFKDENNELPEVQWYKNCKPLLLDNVSFFGVKDKLLVRNVAEEHRGDYICRMSYTFRGKQYPVTRVIQFITIDENKRDRPVILSPRNETIEADPGSMIQLICNVTGQFSDLVYWKWNGSEIEWNDPFLAEDYQFVEHPSTKRKYTLITTLNISEVKSQFYRYPFICVVKNTNIFESAHVQLIYPVPDFKNYLIGGFIILTATIVCCVCIYKVFKVDIVLWYRDSCSGFLPSKASDGKTYDAYILYPKTLGEGSFSDLDTFVFKLLPEVLEGQFGYKLFIYGRDDYVGEDTIEVTNENVKKSRRLIIILVRDMGGFSWLGQSSEEQIAIYNALIQEGIKIVLLELEKIQDYEKMPDSIQFIKQKHGVICWSGDFQERPQSAKTRFWKNLRYQMPAQRRSPLSKHRLLTLDPVRDTKEKLPAATHLPLG tolip_mouse Toll-interacting protein Tollip protein protein mouse Mus musculus Mouse MATTVSTQRGPVYIGELPQDFLRITPTQQQQQIQLDAQAAQQLQYGGTVGTVGRLSITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIQCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLKQGQVEDEWYSLSGRQGDDKEGMINLVMSYTSLPAAMMMPPQPVVLMPTVYQQGVGYVPIAGMPAVCSPGMVPMAMPPPAVAPQPRCNEEDLKAIQDMFPNMDREVIRSVLEAQRGNKDAAINSLLQMGEES myd88_mouse Myeloid differentiation primary response protein MyD88 Myd88 protein protein mouse Mus musculus Mouse MSAGDPRVGSGSLDSFMFSIPLVALNVGVRRRLSLFLNPRTPVAADWTLLAEEMGFEYLEIRELETRPDPTRSLLDAWQGRSGASVGRLLELLALLDREDILKELKSRIEEDCQKYLGKQQNQESEKPLQVARVESSVPQTKELGGITTLDDPLGQTPELFDAFICYCPNDIEFVQEMIRQLEQTDYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDDYLQSKECDFQTKFALSLSPGVQQKRLIPIKYKAMKKDFPSILRFITICDYTNPCTKSWFWTRLAKALSLP q62406-2 Interleukin-1 receptor-associated kinase 1 Irak1 Il1rak Pelle-like protein kinase protein protein mouse Mus musculus Mouse MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLPVSLGPPLPSSAPSSTKSSPESPVSGLQRAHPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGLYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGAKASQSSTVARTSTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEAGVTLKSTQPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQSGDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTTRESSVRSSPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPDFVDIDAIGIEAFMSELFINHI irak1_mouse Interleukin-1 receptor-associated kinase 1 Pelle-like protein kinase Irak1 Il1rak protein protein mouse Mus musculus Mouse MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLPVSLGPPLPSSAPSSTKSSPESPVSGLQRAHPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGLYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGAKASQSSTVARTSTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEAGVTLKSTQPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQSGDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTTRESSVRSSPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKSQGPEESDEFQS il1ap_mouse Interleukin-1 receptor accessory protein Il1rap Interleukin-33 receptot beta chain protein protein mouse Mus musculus Mouse MGLLWYLMSLSFYGILQSHASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSAMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNYTCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEVWWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQKVIPPRYTVELACGFGATVFLVVVLIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNVEEEEFVLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQALLELKAGLENMASRGNINVILVQYKAVKDMKVKELKRAKTVLTVIKWKGEKSKYPQGRFWKQLQVAMPVKKSPRWSSNDKQGLSYSSLKNV il1r1-il1rl1-1 Coimmunoprecipitation reveals interaction between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Toll-interacting protein (Tollip) 175879 n/a 175882 unspecified role unspecified role bait bait N/A 175883 unspecified role unspecified role prey prey physical association physical association false false false Association between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Toll-interacting protein (Tollip) was detected 2 minutes after treatment of EL4 cells with Interleukin 1 and was undetectable after 5 minutes treatment. il1r1-il1rl1 Coimmunoprecipitation reveals interaction between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Myeloid differentiation primary response protein (MyD88) 175879 n/a 175882 unspecified role unspecified role bait bait N/A 175884 unspecified role unspecified role prey prey physical association physical association false false false Association between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Myeloid differentiation primary response protein (MyD88) occurred 30 seconds after treatment of EL4 cells with Interleukin 1 and was detected at all timepoints thereafter. il1r1-il1rl1-2 Coimmunoprecipitation reveals interaction between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Interleukin-1 receptor-associated kinase 1 (IRAK1) 175879 n/a 175882 unspecified role unspecified role bait bait N/A 175885 unspecified role unspecified role prey prey physical association physical association false false false Association between the Interleukin-1 receptor-like 1 precursor (IL-1R1) and the Interleukin-1 receptor-associated kinase 1 (IRAK1) occurred 30 seconds after treatment of EL4 cells with Interleukin 1 and was detected at all timepoints thereafter. tollip-irak1 Coimmunoprecipitation reveals interaction between Interleukin-1 receptor-associated kinase 1 and the Toll-interacting protein 175879 N/A 175883 unspecified role unspecified role bait bait N/A 175886 unspecified role unspecified role prey prey physical association physical association false false false The interaction between Interleukin-1 receptor-associated kinase 1(IRAK1) and the Toll-interacting protein was only detected in EL-4 cells which were not stimulated with Interleukin-1 il_1racp-tollip-1 Coimmunoprecipitation reveals interaction between Interleukin-1 receptor accessory protein and 175880 N/A 175883 unspecified role unspecified role prey prey region VSV tag (YTDIEMNRLGK) tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 175887 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false tollip-irak1 Coimmunoprecipitation reveals interaction between Interleukin-1 receptor-associated kinase 1 and the Toll-interacting protein 175880 N/A 175883 unspecified role unspecified role bait bait region VSV tag (YTDIEMNRLGK) tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 175886 unspecified role unspecified role prey prey n terminal N-terminal domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q62406:p.Thr66Ala Threonine to alanine subsititution mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Tollip binding to the N terminal region of IRAK1 prevents phosphorylation of IRAK1 The 293T cells expressed murine Interleukin receptor 1 (IL-1R1). irak1-myd88 Coimmunoprecipitation reveals interaction between Interleukin-1 receptor-associated kinase 1 and the Myeloid differentiation primary response protein (MyD88) 175880 N/A 175886 unspecified role unspecified role prey prey Q62406:p.Thr66Ala Threonine to alanine subsititution mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 175884 unspecified role unspecified role bait bait death domain death domain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Tollip binding to the N terminal region of IRAK1 prevents phosphorylation of IRAK1 il_1racp-tollip Yeast-2-hybrid reveals interaction between Interleukin-1 receptor accessory protein and 175881 N/A 175883 unspecified role unspecified role prey prey N/A 175887 unspecified role unspecified role bait bait intracellular domain Intracellular domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false