UniProt UniProt http://www.uniprot.org czaplinski-2000-1 Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency. in vitro In vitro pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Czaplinski K., Majlesi N., Banerjee T., Peltz SW. RNA (1355-8382) 2000 ecm32_yeast Putative ATP-dependent RNA helicase ECM32 Extracellular mutant protein 32 Helicase 1 DNA helicase B DNA helicase III Modulator of translation termination protein 1 ECM32 HEL1 MTT1 SYGP-ORF61 YER176W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDFQCRTCSQAYDAEQMMKHLSSTRHKTVFDTSNDEDICCEECQDKNIHQLQIIRFGGEDMVLLCNSCFRKEYSETERPSTSYSLQNGSILKFWEKYVKVRECCCDECGEESNLNANRNGEVLCDKCLPKSNRAKDFVSEKSGRFLYIYLGLNETQNSTRKPRKKGGRRVGRGKKGRKGAKIKKEKKETFEAKISRIAYEVKKENSTIQSSSSSNLRNFKGFKAVESDPVVAAKVSKSETSRSNPGPSNRNKGKGNKANHKKNSGNGIGKEKERKTNIRNNVRNSQPIPEDRKNTNSHVTTNSGGKGKNESVDKHQLPQPKALNGNGSGSTNTTGLKKGKKDHAGQKTKGNDKTGNKNPREAKLNSAGRKNALGKKSNNQPNKGTSRWTIGSDTESSREPSISPNENTTSITKSRNRNKKASKPTLNEKSKTTTMPKKLETKNQEKNNGKTKDGKLIYEEGEPLTRYNTFKSTLSYPDLNTYLNDYSFALFLEQKLENEFVQNFNILWPRNEKDTAFIINVEKNNNSELEKLLPANLLALGRPAFNERQPFFFCTQDEQKVWYIFIKELSIQRGKYVLLVELFSWNNLSLPTKNGSSQFKLLPTSAQTSRILFAMTRITNPKFIDLLLGQKPIKEIYFDNRLKFSSDKLNRSQKTAVEHVLNNSITILQGPPGTGKTSTIEEIIIQVIERFHAFPILCVAASNIAIDNIAEKIMENRPQIKILRILSKKKEQQYSDDHPLGEICLHNIVYKNLSPDMQVVANKTRRGEMISKSEDTKFYKEKNRVTNKVVSQSQIIFTTNIAAGGRELKVIKECPVVIMDEATQSSEASTLVPLSLPGIRNFVFVGDEKQLSSFSNIPQLETSLFERVLSNGTYKNPLMLDTQYRMHPKISEFPIKKIYNGELKDGVTDEQKAWPGVQHPLFFYQCDLGPESRVRSTQRDIVGFTYENKHECVEIVKIIQILMLDKKVPLEEIGVITPYSAQRDLLSDILTKNVVINPKQISMQQEYDEIELFNAAGSQGTAGSLQNNVINIINGLHVATVDSFQGHEKSFIIFSCVRNNTENKIGFLRDKRRLNVALTRAKHGLIVVGNKNVLRKGDPLWKDYITYLEEQEVIFTDLTAY erf3_yeast Eukaryotic peptide chain release factor GTP-binding subunit SUP35 GST1 PNM2 SAL3 SUF12 SUP2 YDR172W YD9395.05 ERF2 ERF-3 Translation release factor 3 Polypeptide release factor 3 Omnipotent suppressor protein 2 G1 to S phase transition protein 1 PSI no more protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGGYYQNYQGYSGYQQGGYQQYNPDAGYQQQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYKNFNYNNNLQGYQAGFQPQSQGMSLNDFQKQQKQAAPKPKKTLKLVSSSGIKLANATKKVGTKPAESDKKEEEKSAETKEPTKEPTKVEEPVKKEEKPVQTEEKTEEKSELPKVEDLKISESTHNTNNANVTSADALIKEQEEEVDDEVVNDMFGGKDHVSLIFMGHVDAGKSTMGGNLLYLTGSVDKRTIEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETEKRRYTILDAPGHKMYVSEMIGGASQADVGVLVISARKGEYETGFERGGQTREHALLAKTQGVNKMVVVVNKMDDPTVNWSKERYDQCVSNVSNFLRAIGYNIKTDVVFMPVSGYSGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHINAPFMLPIAAKMKDLGTIVEGKIESGHIKKGQSTLLMPNKTAVEIQNIYNETENEVDMAMCGEQVKLRIKGVEEEDISPGFVLTSPKNPIKSVTKFVAQIAIVELKSIIAAGFSCVMHVHTAIEEVHIVKLLHKLEKGTNRKSKKPPAFAKKGMKVIAVLETEAPVCVETYQDYPQLGRFTLRDQGTTIAIGKIVKIAE 43912A6D77DFA153 erf1_yeast Eukaryotic peptide chain release factor subunit 1 SUP45 SAL4 SUP1 YBR143C YBR1120 Omnipotent suppressor protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNEVEKNIEIWKVKKLVQSLEKARGNGTSMISLVIPPKGQIPLYQKMLTDEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNTLPKNGLVLYCGDIITEDGKEKKVTFDIEPYKPINTSLYLCDNKFHTEVLSELLQADDKFGFIVMDGQGTLFGSVSGNTRTVLHKFTVDLPKKHGRGGQSALRFARLREEKRHNYVRKVAEVAVQNFITNDKVNVKGLILAGSADFKTDLAKSELFDPRLACKVISIVDVSYGGENGFNQAIELSAEALANVKYVQEKKLLEAYFDEISQDTGKFCYGIDDTLKALDLGAVEKLIVFENLETIRYTFKDAEDNEVIKFAEPEAKDKSFAIDKATGQEMDVVSEEPLIEWLAANYKNFGATLEFITDKSSEGAQFVTGFGGIGAMLRYKVNFEQLVDESEDEYYDEDEGSDYDFI 9B59133EE3B1F61E mtt1-erf3 Interaction detected by GST pull-down 170124 N/A 170125 unspecified role unspecified role prey prey n-terminal FLAG tagged full length mtt1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged monoclonal western monoclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 170126 unspecified role unspecified role bait bait n-terminal GST tagged full length erf3 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false mtt1-erf3-1 Interaction detected by GST pull-down 170124 N/A 170126 unspecified role unspecified role bait bait n-terminal GST tagged erf3 N-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false n-terminus N-terminal regulatory domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 170125 unspecified role unspecified role prey prey n-terminal FLAG tagged full length mtt1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged monoclonal western monoclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false The interaction of the erf3 N-terminal regulatory domain with mtt1 is weaker than for the erf3 C-terminal functional domain with mtt1. mtt1-erf3-2 Interaction detected by GST pull-down 170124 N/A 170125 unspecified role unspecified role prey prey n-terminal FLAG tagged full length mtt1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged monoclonal western monoclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 170126 unspecified role unspecified role bait bait erf3-254 GST tagged erf3 C-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false c-terminus C-terminal functional domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false The interaction of the erf3 N-terminal regulatory domain with mtt1 is weaker than for the erf3 C-terminal functional domain with mtt1. mtt1-erf1 Interaction detected by GST pull-down 170124 N/A 170125 unspecified role unspecified role prey prey n-terminal FLAG tagged full length mtt1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged monoclonal western monoclonal antibody western blot certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 170127 unspecified role unspecified role bait bait n-terminal GST tagged full lentgh erf1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false The mtt1-erf1 interaction is weaker than the mtt1-erf3 interaction.