IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ stahl-1999-1 Tic40, a new "old" subunit of the chloroplast protein import translocon. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stahl T., Glockmann C., Soll J., Heins L. Purified chloroplast inner envelope membranes from pea (Pisum sativum) were were cross-linked using either 0.5mM dithiobissuccinimidyl propionate (DSP) or CuCl2 prior to coimmunoprecipitation. Figure 6. jsoll@bot.uni-kiel.de J. Biol. Chem. (0021-9258) 1999 tic40_pea Protein TIC 40, chloroplastic TIC40 Translocon at the inner envelope membrane of chloroplasts 40 protein protein pea Pisum sativum (Garden pea) Garden pea MENLNLALVSSPKPLLLGHSSSKNVFSGRKSFTFGTFRVSANSSSSHVTRAASKSHQNLKSVQGKVNAHDFASISSSNGQETTSVGVSPQLSPPPPSTVGSPLFWIGIGVGFSALFSVVASRVKKYAMQQAFKSMMGQMNTQNNPFDSGAFSSGPPFPFPMPSASGPATPAGFAGNQSQATSTRSASQSTVTVDIPATKVEAAAPAPDINVKEEVEVKNEPKKSAFVDVSPEETVQKNAFERFKDVDESSSFKEARAPAEASQNGTPFKQGFGDSPSSPSERKSALSVDALEKMMEDPTVQQMVYPYLPEEMRNPSTFKWMMQNPEYRQQLEAMLNNMGGGTEWDSRMMDTLKNFDLNSPDVKQQFDQIGLSPQEVISKIMANPDVAMAFQNPRVQAAIMDCSQNPMSIVKYQNDKEVMDVFNKISELFPGVSGPP ti110_pea Protein TIC110, chloroplastic TIC110 IEP110 Chloroplast inner envelope protein, 110 kDa IAP100 Translocon at the inner envelope membrane of chloroplasts 110 protein protein pea Pisum sativum (Garden pea) Garden pea MNPSTLKPSHTHPSLLLPAPSPLRTQRRRFRVSLPRCSSDTNNPASSSSPPQRPPKELNGIEILVDKLSSPARLATSAVIVAGAVAAGYGLGSRFGGSRNAALGGAVALGAAGGAAAYALNAAAPQVAAVNLHNYVAGFDDPSILTREDIEVIANKYGVSKQDEAFKAEICDIYSEFVSSVIPPGGEELKGDEVDKIVNFKSSLGLDDPDAAAVHMEIGRKLFRQRLEVGDREGGVEQRRAFQKLIYVSNIVFGDASSFLLPWKRVFKVTESQVEVAIRDNAQRLYASKLKSVGRDFDLGKLVTLKETQSLCCLSDELAENLFREHARKLVEENISVALGILKSRTRAVPGVSQVVEEIEKVLAFNDLLISFKNHSDIDRLARGVGPVSLVGGEYDADRKIEDLKLLYRAYVSDALSSGRMEDNKFAALNQLKNIFGLGKREAEAILLDITRKVYRKRLGQTVSSGELEMADSKAAFLQNLCDELHFDPQKASELHEEIYRQKLQQCVADGELTDENVAALLKLRVMLCVPQQTVEAAHAEICGNLFEKIVKDAIASGVDGYDDETKKSVRKAAHGLRLTKETALSIASKAVRKMFITYVKRSRSAKGNGESAKELKKLIAFNTLVVTKLVEDIKGESPDVKIEEPKIEEPEEIRESEEYEMRITSDTQENKTGQRACRKDGKAWSDRITLKDDLPEKDRADLYKTFLTYCLTGDVVRIPFGVEIKKKKDDTEYIYLNQLGGILGLTGKVIMDVHRGLAEQAFRKQAEVLLADGQLTKARVEQLGKMQKEIGLSQEYAQKIIKNITTTKMAAAIETAVTQGKLNMKQIRELKESNVDLDSMVSVSLRETIFKKTVGDIFSSGTGEFDEEEVYEKIPLDLNINKEKARGVVCELAQNRLSNSLIQAVALLRQRNHKGVVFSLNNLLACDKAVPSQTLSWEVSEELSDLYTIYLKSDPSPEKLSRLQYLLGINDSTAAALRDSEDSLLETAEEEKFVF This sequence has been withdrawn from Uniprot. E36649FF7A0C2680 toc40-toc110 Anti bait coimmunoprecipitaion reveals interaction between the Toc40 and Toc110 proteins 209712 N/A 209713 unspecified role unspecified role bait bait pea Pisum sativum (Garden pea) Garden pea N/A 209714 unspecified role unspecified role prey prey pea Pisum sativum (Garden pea) Garden pea physical association physical association false false false