IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ peters-1997-1 Differential association of syntrophin pairs with the dystrophin complex. mouse-skeletal-muscle Mus musculus skeletal muscle. skeletal_muscle Skeletal muscle coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Peters MF., Adams ME., Froehner SC. J. Cell Biol. (0021-9525) 1997 peters-1997-2 Differential association of syntrophin pairs with the dystrophin complex. mouse-skeletal-muscle Mus musculus skeletal muscle. skeletal_muscle Skeletal muscle imaging technique imaging technique microscopy immunostaining immunostaining Peters MF., Adams ME., Froehner SC. Tissue:Skeletal muscle from transgenic mdx mice expressing a dystrophin construct with a deletion of the syntrophin binding site (exons 71-74 of the dystrophin gene) J. Cell Biol. (0021-9525) 1997 peters-1997-3 Differential association of syntrophin pairs with the dystrophin complex. mouse-skeletal-muscle Mus musculus skeletal muscle. skeletal_muscle Skeletal muscle coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Peters MF., Adams ME., Froehner SC. Tissue:Skeletal muscle from transgenic mdx mice expressing a dystrophin construct with a deletion of the syntrophin binding site (exons 71-74 of the dystrophin gene)but the delta 71-74 dystrophin is targetted to the sarcolemma and normal levels of membrane-bound dystrophin are present. J. Cell Biol. (0021-9525) 1997 dmd_mouse Dystrophin Dmd protein protein mouse Mus musculus Mouse MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIDNLFSDLQDGKRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKTIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTSSWSDGLALNALIHSHRPDLFDWNSVVSQHSATQRLEHAFNIAKCQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRTSSKVTREEHFQLHHQMHYSQQITVSLAQGYEQTSSSPKPRFKSYAFTQAAYVATSDSTQSPYPSQHLEAPRDKSLDSSLMETEVNLDSYQTALEEVLSWLLSAEDTLRAQGEISNDVEEVKEQFHAHEGFMMDLTSHQGLVGNVLQLGSQLVGKGKLSEDEEAEVQEQMNLLNSRWECLRVASMEKQSKLHKVLMDLQNQKLKELDDWLTKTEERTKKMEEEPFGPDLEDLKCQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWIVLQDILLKWQHFTEEQCLFSTWLSEKEDAMKNIQTSGFKDQNEMMSSLHKISTLKIDLEKKKPTMEKLSSLNQDLLSALKNKSVTQKMEIWMENFAQRWDNLTQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREQIMVKHAQEELPPPPPQKKRQITVDSELRKRLDVDITELHSWITRSEAVLQSSEFAVYRKEGNISDLQEKVNAIAREKAEKFRKLQDASRSAQALVEQMANEGVNAESIRQASEQLNSRWTEFCQLLSERVNWLEYQTNIITFYNQLQQLEQMTTTAENLLKTQSTTLSEPTAIKSQLKICKDEVNRLSALQPQIEQLKIQSLQLKEKGQGPMFLDADFVAFTNHFNHIFDGVRAKEKELQTIFDTLPPMRYQETMSSIRTWIQQSESKLSVPYLSVTEYEIMEERLGKLQALQSSLKEQQNGFNYLSDTVKEMAKKAPSEICQKYLSEFEEIEGHWKKLSSQLVESCQKLEEHMNKLRKFQNHIKTLQKWMAEVDVFLKEEWPALGDAEILKKQLKQCRLLVGDIQTIQPSLNSVNEGGQKIKSEAELEFASRLETELRELNTQWDHICRQVYTRKEALKAGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEALQKETKVKLLTETVNSVIAHAPPSAQEALKKELETLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTMENVPAGPEEITEVLESLENLMHHSEENPNQIRLLAQTLTDGGVMDELINEELETFNSRWRELHEEAVRKQKLLEQSIQSAQEIEKSLHLIQESLEFIDKQLAAYITDKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKDANQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLEESKMILDEVKMHLPALETKSVEQEVIQSQLSHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNVLTEWLAATDTELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKAHLKSVTELGESLKMVLGKKETLVEDKLSLLNSNWIAVTSRVEEWLNLLLEYQKHMETFDQNIEQITKWIIHADELLDESEKKKPQQKEDILKRLKAEMNDMRPKVDSTRDQAAKLMANRGDHCRKVVEPQISELNRRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVNELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDEIEKKLASLPEPRDERKLKEIDRELQKKKEELNAVRRQAEGLSENGAAMAVEPTQIQLSKRWRQIESNFAQFRRLNFAQIHTLHEETMVVTTEDMPLDVSYVPSTYLTEISHILQALSEVDHLLNTPELCAKDFEDLFKQEESLKNIKDNLQQISGRIDIIHKKKTAALQSATSMEKVKVQEAVAQMDFQGEKLHRMYKERQGRFDRSVEKWRHFHYDMKVFNQWLNEVEQFFKKTQNPENWEHAKYKWYLKELQDGIGQRQAVVRTLNATGEEIIQQSSKTDVNILQEKLGSLSLRWHDICKELAERRKRIEEQKNVLSEFQRDLNEFVLWLEEADNIAITPLGDEQQLKEQLEQVKLLAEELPLRQGILKQLNETGGAVLVSAPIRPEEQDKLEKKLKQTNLQWIKVSRALPEKQGELEVHLKDFRQLEEQLDHLLLWLSPIRNQLEIYNQPSQAGPFDIKEIEVTVHGKQADVERLLSKGQHLYKEKPSTQPVKRKLEDLRSEWEAVNHLLRELRTKQPDRAPGLSTTGASASQTVTLVTQSVVTKETVISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQIQWDEVQEQLQNRRQQLNEMLKDSTQWLEAKEEAEQVIGQVRGKLDSWKEGPHTVDAIQKKITETKQLAKDLRQRQISVDVANDLALKLLRDYSADDTRKVHMITENINTSWGNIHKRVSEQEAALEETHRLLQQFPLDLEKFLSWITEAETTANVLQDASRKEKLLEDSRGVRELMKPWQDLQGEIETHTDIYHNLDENGQKILRSLEGSDEAPLLQRRLDNMNFKWSELQKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNAEWDKLNLRSADWQRKIDEALERLQELQEAADELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVNRVNDLAHQLTTLGIQLSPYNLSTLEDLNTRWRLLQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNAPGKPMREDTM snta1_mouse Alpha-1-syntrophin Snta1 Snt1 59 kDa dystrophin-associated protein A1 acidic component 1 Syntrophin-1 protein protein mouse Mus musculus Mouse MASGRRAPRTGLLELRCGAGSGAGGERWQRVLLSLAEDALTVSPADGEPGPEPEPAQLNGAAEPGAAPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQALKKTGKEVVLEVKYMKEVSPYFKNSAGGTSVGWDSPPASPLQRQPSSPGPQPRNLSEAKHVSLKMAYVSRRCTPTDPEPRYLEICAADGQDAVFLRAKDEASARSWAGAIQAQIGTFIPWVKDELQALLTATGTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLFYCSLPQSREALSRPTRTAPLIATSSAHRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGIQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAMLLRQPFEKLQMSSDDGTSLLFLDFGGAEGEIQLDLHSCPKTMVFIIHSFLSAKVTRLGLLA sntb1_mouse Beta-1-syntrophin Sntb1 Snt2b1 59 kDa dystrophin-associated protein A1 basic component 1 Syntrophin-2 protein protein mouse Mus musculus Mouse MAVAAAAVAAPAGGGGARAQRSGLLEVLVRDRWHKVLVNLSEDALVLSCEEGAAAYNGIGAATNGSFCRGSGTGHPVPGVAQAPDSPAGVRTAFTDLPEQVPESISNQKRGVKVLKQELGGLGISIKGGKENKMPILISKIFKGLAADQTQALYVGDAILSVNGADLRDATHDEAVQALKRAGKEVLLEVKYMREATPYVKKGSPVSEIGWETPPPESPRLGGGSAEPLSSQSFSFHRDRKSIPLKMCYVTRNMTLADPENRQLEIHSPDAKHTVILRSKDSATAQAWFSAIHSNAGDLLTRVVADIREQLGKTGIAGSREIRHLGWLAEKVPGESEKQWKPALVVLTEKDLLIYDSMPRRKEAWFSPVHSYPLLATRLVHSGPGKGSPQAGMDLSFATRTGTKQGIETHLFRAEISRDLSHWTRSIVQGCHNSAELTAEITTACTYRNQECRLTIHYDNGFSISTEPQDGAFPKTIIQSPYEKLKMSSDDGIRMLYLDFGGKEGEIQLDLHSCPKPIVFIIHSFLSAKITRLGLVA q8c7r5_mouse protein protein mouse Mus musculus Mouse MAKYGDLEARPDDGQNEFSDIIKSRSDEHNDVQKKTFTKWINARFSKSGKPPISDMFSDLKDGRKLLDLLEGLTGTSLPKERGSTRVHALNNVNRVLQVLHQNNVDLVNIGGTDIVDGNPKLTLGLLWSIILHWQVKDVMKDIMSDLQQTNSEKILLSWVRQTTRPYSQVNVLNFTTSWTDGLAFNAVLHRHKPDLFSWDRVVKMSPIERLEHAFSKAHTYLGIEKLLDPEDVAVHLPDKKSIIMYLTSLFEVLPQQVTIDAIREVETLP sntb2_mouse Beta-2-syntrophin Sntb2 Snt2b2 59 kDa dystrophin-associated protein A1 basic component 2 Syntrophin-3 Syntrophin-like protein protein mouse Mus musculus Mouse MAVWTRATKAGLVELLLRERWVRVVAELSGESLSLTGDAAAVEPEPPAAAFNGLPNGGGGESLPGSPNRGLGPPSPPAPPRGPAGEASASPPVRRVRVVKQEAGGLGISIKGGRENRMPILISKIFPGLAADQSRALRLGDAILSVNGTDLRQATHDQAVQALKRAGKEVLLEVKFIREVTPYIKKPSLVSDLPWEGASPQSPSFSGSEDSGSPKHQNTTKDRKVIPLKMCFAARNLSMPDLENRLIELHSPDSRNTLILRCKDTATAHSWFVAIHTNIMALLPQVLAELNAMLGATSTAGGSKEVKHIAWLAEQAKLDGGRQQWRPVLMAVTEKDLLLYDCMPWTRDAWASPCHSYPLVATRLVHSGSGCRSPSLGSDLTFATRTGSRQGIEMHLFRVETHRDLSTWTRILVQGCHAAAELIKEVSLGCTLSGQEVRFTVHYEHGFTITRDNGGASSILYRYPFERLKMSADDGIRNLYLDFGGPEGELTMDLHSCPKPIVFVLHTFLSAKVTRMGLLV dtna_mouse Dystrobrevin alpha Dtna Dtn Alpha-dystrobrevin protein protein mouse Mus musculus Mouse MIEDSGKRGNTMAERRQLFAEMRAQDLDRIRLSTYRTACKLRFVQKKCNLHLVDIWNVIEALRENALNNLDPNIELNVARLEAVLSTIFYQLNKRMPTTHQIHVEQSISLLLNFLLAAFDPEGHGKISVFAVKMALATLCGGKIMDKLRYIFSMISDSSGVMVYGRYDQFLREVLKLPTAVFEGPSFGYTEQSARSCFSQQKKVTLNGFLDTLMSDPPPQCLVWLPLLHRLANVENVFHPVECSYCHSESMMGFRYRCQQCHNYQLCQDCFWRGHAGGSHSNQHQMKEYTSWKSPAKKLTNALSKSLSCASSREPLHPMFPDQPEKPLNLAHIVPPRPVTSMNDTLFSHSVPSSGSPFITRSSPPKDSEVEQNKMLARAAPAFLKGRGIQYSLNVADRLADEHVLIGLYVNMLRNDPPCMLESSNRLDEEHRLIARYAARLAAESSSSQPTQQRSAPDISFTIDANKQQRQLIAELENKNREILQEIQRLRVEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKEEELKQGTQGASSPRSSPSHTISRPIPMPIRSASACPTPTHTPQDSLTGVGGDVQEAFAQSSRRNLRSDLLVAADSITNTMSSLVKELNSEVASETESTVDSEFSRPQFEDLAPSPTSEKAFLAQIHSRKPGYIHGGAASTTHGDMVPEDGDPYTQPEDGNYENESVRQLENELQLEEYLKQKLQDEAYQVSLQG dmd-sntb2 dystrophin-alpha-1 and beta-1 syntrophin interacation 98635 N/A 98638 unspecified role unspecified role bait bait N/A 98639 unspecified role unspecified role prey prey N/A 98640 unspecified role unspecified role prey prey association association false false false Immunoaffinity purification from skeletal muscle. In mdx mice (where dystrophin is deficient due to a premature stop codon in exon 23 of the gene) sarcolemmal staining of alpha-1 and beta-1 syntrophin is lost. This result supports the association of these proteins in wt tissue. q8c7r5-sntb1 utrophin-beta-1-syntrophin-beta-2-syntrophin interaction. 98635 N/A 98641 unspecified role unspecified role bait bait N/A 98642 unspecified role unspecified role prey prey N/A 98640 unspecified role unspecified role prey prey association association false false false Immnuno-affinity purification from skeletal muscle. snta1-dmd mouse alpha-1-syntrophin-dystrophin interaction 98635 N/A 98639 unspecified role unspecified role bait bait N/A 98638 unspecified role unspecified role prey prey physical association physical association false false false Immunoaffinity purification from skeletal muscle extracts. sntb1-q8c7r5 beta-1-syntrophin-utrophin-dysrophin interaction. 98635 N/A 98641 unspecified role unspecified role prey prey N/A 98640 unspecified role unspecified role bait bait N/A 98638 unspecified role unspecified role prey prey association association false false false The amount of co-purifying Dystrophin was very low compared with that of Utrophin. sntb2-q8c7r5 mouse beta-2-syntrophin-dystrophin intraction. 98635 N/A 98642 unspecified role unspecified role bait bait N/A 98638 unspecified role unspecified role prey prey N/A 98641 unspecified role unspecified role prey prey association association false false false Amount of dystrophin detected very low compared with utrophin. dmd-snta1 Dystrobrevin-Alpha-1-syntrophin-Beta-1-syntrophin interaction 98636 N/A 98639 unspecified role unspecified role neutral component neutral component N/A 98643 unspecified role unspecified role neutral component neutral component N/A 98640 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false dtna-snta1 Immunoprecipitation of Dystrobrevin complexes from mouse skeletal muscle. 98637 N/A 98643 unspecified role unspecified role bait bait N/A 98638 unspecified role unspecified role prey prey N/A 98640 unspecified role unspecified role prey prey N/A 98639 unspecified role unspecified role prey prey association association false false false The authors present evidence that the interaction between dystrobrevin and dystrophin is mediated by their coiled-coil regions.