<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2004-10-01+01:00">
            <names>
                <shortLabel>IntAct</shortLabel>
                <fullName>European Bioinformatics Institute</fullName>
                <alias typeAc="MI:1041" type="synonym">IntAct</alias>
                <alias typeAc="MI:1041" type="synonym">intact</alias>
            </names>
            <bibref>
                <xref>
                    <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
            </bibref>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="22121220" refType="method reference" refTypeAc="MI:0357"/>
            </xref>
            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
                <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
                <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
                <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
                <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
                <attribute name="url">http://www.ebi.ac.uk/</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="143391">
                <names>
                    <shortLabel>cohlberg-1995-1</shortLabel>
                    <fullName>Neurofilament protein heterotetramers as assembly intermediates.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="7721855" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-444248" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>affinity chrom</shortLabel>
                        <fullName>affinity chromatography technology</fullName>
                        <alias typeAc="MI:0303" type="go synonym">Affinity purification</alias>
                        <alias typeAc="MI:1041" type="synonym">Affinity purification</alias>
                        <alias typeAc="MI:1041" type="synonym">affinity chrom</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0004" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-86" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>predetermined</shortLabel>
                        <fullName>predetermined participant</fullName>
                        <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Cohlberg JA., Hajarian H., Tran T., Alipourjeddi P., Noveen A.</attribute>
                    <attribute name="exp-modification" nameAc="MI:0627">The components of the interaction (complex) were separated by electrophoretic native gel in 2M urea</attribute>
                    <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">1995</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="143392">
                <names>
                    <shortLabel>nfl_bovin</shortLabel>
                    <fullName>Neurofilament light polypeptide</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">Neurofilament triplet L protein</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">68 kDa neurofilament protein</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Micro glutamic acid-rich protein</alias>
                    <alias typeAc="MI:0301" type="gene name">NEFL</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P02548" version="SP_68" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0008090" secondary="retrograde axonal transport" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0008089" secondary="anterograde axonal transport" version="SP_171"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q17QQ0" version="SP_68" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005200" secondary="structural constituent of cytoskeleton" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0000226" secondary="microtubule cytoskeleton organization" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0060074" secondary="synapse maturation" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0033693" secondary="neurofilament bundle assembly" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031133" secondary="regulation of axon diameter" version="SP_171"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039008" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0099184" secondary="structural constituent of postsynaptic intermediate filament cytoskeleton" version="SP_171"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006821" version="SP_171"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P79127" version="SP_68" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAT00000029264" version="SP_171" refType="transcript"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0008022" secondary="protein C-terminus binding" version="SP_171"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_776546.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031594" secondary="neuromuscular junction" version="SP_171"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-444335" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005882" secondary="intermediate filament" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0060052" secondary="neurofilament cytoskeleton organization" version="SP_171"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAP00000029264" version="SP_171" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0040011" secondary="locomotion" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" secondary="identical protein binding" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0050772" secondary="positive regulation of axonogenesis" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:1904115" secondary="axon cytoplasm" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0019896" secondary="axonal transport of mitochondrion" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0099160" secondary="postsynaptic intermediate filament cytoskeleton" version="SP_171"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027692" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0099182" secondary="presynaptic intermediate filament cytoskeleton" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0045109" secondary="intermediate filament organization" version="SP_171"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018039" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0043524" secondary="negative regulation of neuron apoptotic process" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0048812" secondary="neuron projection morphogenesis" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030674" secondary="protein-macromolecule adaptor activity" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0045110" secondary="intermediate filament bundle assembly" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0014012" secondary="peripheral nervous system axon regeneration" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030424" secondary="axon" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0098981" secondary="cholinergic synapse" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0098685" secondary="Schaffer collateral - CA1 synapse" version="SP_171"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005883" secondary="neurofilament" version="SP_171"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAG00000021949" version="SP_171" refType="gene"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0050885" secondary="neuromuscular process controlling balance" version="SP_171"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="9913">
                    <names>
                        <shortLabel>bovin</shortLabel>
                        <fullName>Bos taurus (Bovine)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Bovine</alias>
                    </names>
                </organism>
                <sequence>MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLESLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSLTTGYTQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEAEAEAEAEAEAEAEEEEGAQEEEAAKEDAEEAKEEEGGEGEEAEETKEAEEEEKKDEGAGEEQATKKKD</sequence>
            </interactor>
            <interactor id="143393">
                <names>
                    <shortLabel>nfm_bovin</shortLabel>
                    <fullName>Neurofilament medium polypeptide</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">Neurofilament triplet M protein</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">160 kDa neurofilament protein</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Neurofilament 3</alias>
                    <alias typeAc="MI:0301" type="gene name">NEFM</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">NEF3</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">NFM</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="O77788" version="SP_54" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005198" secondary="structural molecule activity" version="SP_130"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005883" secondary="neurofilament" version="SP_130"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039008"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018039"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027697"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030424" secondary="axon" version="SP_130"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0033693" secondary="neurofilament bundle assembly" version="SP_130"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006821"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-444343" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002957"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="9913">
                    <names>
                        <shortLabel>bovin</shortLabel>
                        <fullName>Bos taurus (Bovine)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Bovine</alias>
                    </names>
                </organism>
                <sequence>MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLDGGSGPGGDYKLSRSNEKEQIQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPTAKSPTAKSPVAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAPKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKPKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction id="143394">
                <names>
                    <shortLabel>nfl-nfm-1</shortLabel>
                    <fullName>complex between NFL and NFM detected by electrophorectic native gel in urea 2M</fullName>
                </names>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-444354" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>143391</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="143395">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-444355" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>143392</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>neutral component</shortLabel>
                                    <fullName>neutral component</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                    <participant id="143396">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-444356" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>143393</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>neutral component</shortLabel>
                                    <fullName>neutral component</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="comment" nameAc="MI:0612">NFL also form a complex with NFH</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
