IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wigelsworth-2004-2 Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 J. Biol. Chem. (0021-9258) 2004 wigelsworth-2004-1 Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 J. Biol. Chem. (0021-9258) 2004 wigelsworth-2004-3 Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wigelsworth DJ., Krantz BA., Christensen KA., Lacy DB., Juris SJ., Collier RJ. fig 2 J. Biol. Chem. (0021-9258) 2004 antr2_human Anthrax toxin receptor 2 Capillary morphogenesis gene 2 protein ANTXR2 CMG2 protein protein human Homo sapiens Human MVAERSPARSPGSWLFPGLWLLVLSGPGGLLRAQEQPSCRRAFDLYFVLDKSGSVANNWIEIYNFVQQLAERFVSPEMRLSFIVFSSQATIILPLTGDRGKISKGLEDLKRVSPVGETYIHEGLKLANEQIQKAGGLKTSSIIIALTDGKLDGLVPSYAEKEAKISRSLGASVYCVGVLDFEQAQLERIADSKEQVFPVKGGFQALKGIINSILAQSCTEILELQPSSVCVGEEFQIVLSGRGFMLGSRNGSVLCTYTVNETYTTSVKPVSVQLNSMLCPAPILNKAGETLDVSVSFNGGKSVISGSLIVTATECSNGIAAIIVILVLLLLLGIGLMWWFWPLCCKVVIKDPPPPPAPAPKEEEEEPLPTKKWPTVDASYYGGRGVGGIKRMEVRWGDKGSTEEGARLEKAKNAVVKIPEETEEPIRPRPPRPKPTHQPPQTKWYTPIKGRLDALWALLRRQYDRVSLMRPQEGDEVCIWECIEKELTA pag_bacan Protective antigen PA-83 Anthrax toxins translocating protein pagA pag pXO1-110 BXA0164 GBAA_pXO1_0164 protein protein bacan Bacillus anthracis MKKRKVLIPLMALSTILVSSTGNLEVIQAEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG paga-antxr2-3 FRET-based titration : human receptor CMG2 (antrx2) and the protective antigen of anthrax toxin (PA-83) 164515 N/A 164518 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 164519 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 1.0 direct interaction direct interaction false false false At 2mM Mg2+, ka= 5.3E4 M-1 s-1 and kd = 9.2E-6 s-1 paga-antxr2-4 FRET-based titration : human receptor CMG2 (antrx2) and the protective antigen of anthrax toxin (PA-63) 164515 N/A 164518 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore CMG2 (antrx2) is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 N/A 164519 unspecified role unspecified role neutral component neutral component P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false fluorophore PA-63 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 7.0 direct interaction direct interaction false false false paga-paga-2 FRET: the heptamerization of protective antigen (PA-63) requires anthrax lethal factor (LF) 164515 N/A 164519 unspecified role unspecified role neutral component neutral component P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false fluorophore alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 1.0 N/A 164519 unspecified role unspecified role neutral component neutral component pa-63 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 6.0 direct interaction direct interaction false false false A mixture of fluorescently labeled PA-63 on E762C is used (donor AF488 and acceptor) to measure heptamerization Addition of anthrax lethal factor (LF) VWFA protein fragment (34-296) is required for PA-63 heptamerization heptamerization reaction profiles fit to a second order rate with rate constants of 8.8 x 10-4 and 7.8 x 10-4 s-1 in the presence and absence of CMG2, respectively. paga-antxr2-7 FRET-based titration : human receptor CMG2 (antrx2) and the protective antigen of anthrax toxin (PA-83) 164515 N/A 164519 unspecified role unspecified role neutral component neutral component undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false pa-83-x binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false fluorophore-x PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 1.0 N/A 164519 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P13423:p.Glu762Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore PA-83 is modified for AF488 fluorophore attachment : E762C (on PAGA sequence) alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 7.0 N/A 164518 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P58335:p.Arg40Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 7.0 N/A 164518 unspecified role unspecified role neutral component neutral component vwfa-x binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false fluorophore-x CMG2 is modified for AF546 fluorophore attachment : R40C alexa 546 label alexa 546 label certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 direct interaction direct interaction false false false stoechiometry 1:1 or 7:7 At 2mM Mg2+, ka= 5.3E4 M-1 s-1 and kd = 9.2E-6 s-1 paga-antxr2-2 istothermal titration calorimetry : human receptor CMG2 (antrx2) and the protective antigen of anthrax toxin (PA-83) 164516 N/A 164519 unspecified role unspecified role neutral component neutral component pa-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis Stoichiometry: 1.0 N/A 164518 unspecified role unspecified role neutral component neutral component vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) Stoichiometry: 1.0 physical association physical association false false false stoichiometry 1:1 paga-antxr2-5 surface plasma resonance : human receptor CMG2 (antrx2) and the protective antigen of anthrax toxin (PA-83) 164517 N/A 164518 unspecified role unspecified role prey prey vwfa binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 164519 unspecified role unspecified role bait bait pa-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false bacan Bacillus anthracis direct interaction direct interaction false false false At 1 mM Ca2+, ka= 1.7.10E5 M-1 s-1 and kd= 7.10E-5 s-1