IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nakamura-2004-3 Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. drome-ovary Drosophila melanogaster ovary ovary Ovary coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Dev. Cell (1534-5807) 2004 nakamura-2004-1 Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Dev. Cell (1534-5807) 2004 nakamura-2004-2 Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Dev. Cell (1534-5807) 2004 ddx6_drome ATP-dependent RNA helicase me31b me31B CG4916 Maternal expression at 31B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMTEKLNSGHTNLTSKGIINDLQIAGNTSDDMGWKSKLKLPPKDNRFKTTDVTDTRGNEFEEFCLKRELLMGIFEKGWERPSPIQEAAIPIALSGKDVLARAKNGTGKTGAYCIPVLEQIDPTKDYIQALVMVPTRELALQTSQICIELAKHLDIRVMVTTGGTILKDDILRIYQKVQLIIATPGRILDLMDKKVADMSHCRILVLDEADKLLSLDFQGMLDHVILKLPKDPQILLFSATFPLTVKNFMEKHLREPYEINLMEELTLKGVTQYYAFVQERQKVHCLNTLFSKLQINQSIIFCNSTQRVELLAKKITELGYCCYYIHAKMAQAHRNRVFHDFRQGLCRNLVCSDLFTRGIDVQAVNVVINFDFPRMAETYLHRIGRSGRFGHLGIAINLITYEDRFDLHRIEKELGTEIKPIPKVIDPALYVANVGASVGDTCNNSDLNNSANEEGNVSK if4e_drome Eukaryotic translation initiation factor 4E1 eIF4E1 Eif4e eIF-4E eIF-4F 25 kDa subunit mRNA cap-binding protein CG4035 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSDFHRMKNFANPKSMFKTSAPSTEQGRPEPPTSAAAPAEAKDVKPKEDPQETGEPAGNTATTTAPAGDDAVRTEHLYKHPLMNVWTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKDTMVKQGSNVKSIYTL cup_drome Protein cup cup fs(2)cup Oskar ribonucleoprotein complex 147 kDa subunit CG11181 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQMAEAEQENGAGALKIATNAGATDRPAHQQLPLPVEDQQDEVLTPAEKGKFEYPPPPPPPTPVQAPLATKATALNASQEHDDDEANSEKWEDPCAPPPPPPLPTSAFLATGLGYLKLPAFKLKDALEKAITKLEANKRTLKASPESSRSIKNKNVVALEMLPRRSNPETIGDGSMLASTSTAVMLQTKKPAVIVEMERRCKIINLLAKQNQILESISGEAIPMHGPSKHLHEDEGLTLQVLSARASTPYTQPSSMLSCTAVSCDLEHDSPRKQVASKEAVPEQQSSQVQQKRPPSTGIHKPGSLRAPKAVRPTTAPVVSSKPVKSYTRSRLMDIRNGMFNALMHRSKESFVMPRIATCDDIELEGRLRRMNIWRTSDGTRFRTRSTTANLNMNNNNNNECMPAFFKNKNKPNLISDESIIQSQPPQPQTEFQDPAIVNQRRIGSGRLNHSKWGYNDEDYHSYHNGKSQHMEEVNSKNSKNMTVLQFFDNGEISSQPQRRPNTPVMGMSINRSENDTLHSNESSEDLSRANENYVKRVMSGFLVVSKPKSRDVEDRHHRRYRNQNEEPEWFSCGPTSRLDTIELCGFDEDEEKMLKEGNKNHGLGETERETSKQKMDHKYKWTHAEPMGRSKYMPKHDTNNNHNVENMNNVMATEHQQQKEEKRPGSGRSFQFDKFNQSQQNYESSSYVNHQQPPQTQPQQMQQQSNTNTNNSKFMSFFANEGNSSSSSLNEFFKQAINQGHGNNPEQPKSLGHIGQMPSVDQLEAKWRRNSLNNVGETANKQTDNFQKLIGSLSSAKPQSQAVGYDAISNFIMQQQQYQQQQQKQHLIIQQQQQHTAFLASLQLKAILGRADTQLLLLRLTKGEISKHGLLVQLANPRLTDMDREAITAVLQFTNTQQQQQQHKQQLDMLSSTVIASQLQNLHNLAIVQQTLAARQQPQHNPQTQAPHQLSQEDLQAHANVIMRNAVMKRKIEEQTSKLINGGAKHQAQQQYLNRGQQRQARPDANSNALLHALISGGGNNHASGYPMNGQPQKHHSNLRFGDNQNFQSFESNQPHFATQYKQQYQQSQQQHPHQQPQQLNSLHQNNAGAVNSFNKAQMQAQSAISMLPNSGDEFH o18409_drome bru1 aret bruno CG31762 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLSSLDALAGKIATATPGTGAVTADSKNTQSLHHHHRLDYDYSAVEIEPPAEQLANSPRSERERLQQAQQAYEQQAQAELEFGLAEVDAKLTQLKSSHSLQHHHSHHHQLQRHTALHQQQQQLHHHQPHHNHHHHHPYQRPRPTSPPQQQTSTYQQQLSQLAALQDHHDLLQTQQELFHKQLATMQEYQRERERERERERERERERERDRSSFIDNSDYDSQTEFKICLRDSAFGMSDKSSSATNSLPNSPIHSNNNNPSLLNNNNNNSNGTSSNNSLNVGNNSSNPSLGGTNSNALVSVGSNGIMSAAGLVNNNNNPCSANRNVVAMVDDDACFRLDTDATVTYGEKEPDPDNIKMFVGQVPKSMDESQLREMFEEYGAVHSINVLRDKATGISKGCCFVTFYTRRAALKAQDALHNVKTLNGMYHPIQMKPADSENRNERKLFVGMLNKKLNENDVRKLFEVHGAIEECTVLRDQNGQSKGCAFVTFATKHAAISAIKVTLSQNKIMEGCTSPLVVKFADTQKEKEQKKIQQIQANLWNLASNINIPLGQTATSVTTPILPNPAQQPSPVLGADAITPASIQLLQQLQAVGLQHQLLQALTGLGAQQSSSATDTSAVAAAGLLTPMTVQNLAAIAAMTQPSLSNAAAAAARATSPGSAQLTNTAALLWSDPNPMASAYMSTAAGLPQFGSASALSTSPLASVALSAAAAAAAGKQIEGPEGCNLFIYHLPQEFTDTDLASTFLPFGNVISAKVFIDKQTSLSKCFGFVSFDNPDSAQVAIKAMNGFQVGTKRLKVQLKKPKDSKPY cup-eif4e-2 Interaction detected by coIP 103225 N/A 103228 unspecified role unspecified role bait bait in vitro In vitro N/A 103229 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary N/A 103230 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary association association false false false Similar interaction was observed using anti eIF4E. The interaction was RNase sensitive. cup-eif4e-3 Interaction detected by CoIP 103225 N/A 103231 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary N/A 103228 unspecified role unspecified role bait bait in vitro In vitro N/A 103230 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary N/A 103229 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary association association false false false This complex was RNase sensitive. However, the complex between eIF4E-Cup-Bru is RNase resistant cup-aret-1 Interaction detected by Y2D 103226 N/A 103230 unspecified role unspecified role bait bait N/A 103231 unspecified role unspecified role prey prey physical association physical association false false false AA 821-1132 of Cup used as bait. AA 320-520 of Bru used as prey. cup-eif4e-1 Interaction detected by GST-pulldown 103227 N/A 103230 unspecified role unspecified role prey prey in vitro In vitro N/A 103229 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Full length cup cDNA was used to synthesize Cup protein in vitro using TNT T7 Quick kit. GST-EIF4E were expressed in E. coli. The interaction was RNase resistant.