IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nelson-2004-2 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 imex curation Only protein-protein interactions nelson-2004-6 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. in vitro In vitro pull down pull down autoradiography autoradiography Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 imex curation Only protein-protein interactions nelson-2004-3 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. pull down pull down western blot western blot Immuno blot Immuno blot Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 imex curation Only protein-protein interactions nelson-2004-5 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 imex curation Only protein-protein interactions nelson-2004-1 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 Only protein-protein interactions imex curation nelson-2004-4 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Nelson MR., Leidal AM., Smibert CA. c.smibert@utoronto.ca EMBO J. (0261-4189) 2004 imex curation Only protein-protein interactions cup_drome Protein cup cup fs(2)cup Oskar ribonucleoprotein complex 147 kDa subunit CG11181 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQMAEAEQENGAGALKIATNAGATDRPAHQQLPLPVEDQQDEVLTPAEKGKFEYPPPPPPPTPVQAPLATKATALNASQEHDDDEANSEKWEDPCAPPPPPPLPTSAFLATGLGYLKLPAFKLKDALEKAITKLEANKRTLKASPESSRSIKNKNVVALEMLPRRSNPETIGDGSMLASTSTAVMLQTKKPAVIVEMERRCKIINLLAKQNQILESISGEAIPMHGPSKHLHEDEGLTLQVLSARASTPYTQPSSMLSCTAVSCDLEHDSPRKQVASKEAVPEQQSSQVQQKRPPSTGIHKPGSLRAPKAVRPTTAPVVSSKPVKSYTRSRLMDIRNGMFNALMHRSKESFVMPRIATCDDIELEGRLRRMNIWRTSDGTRFRTRSTTANLNMNNNNNNECMPAFFKNKNKPNLISDESIIQSQPPQPQTEFQDPAIVNQRRIGSGRLNHSKWGYNDEDYHSYHNGKSQHMEEVNSKNSKNMTVLQFFDNGEISSQPQRRPNTPVMGMSINRSENDTLHSNESSEDLSRANENYVKRVMSGFLVVSKPKSRDVEDRHHRRYRNQNEEPEWFSCGPTSRLDTIELCGFDEDEEKMLKEGNKNHGLGETERETSKQKMDHKYKWTHAEPMGRSKYMPKHDTNNNHNVENMNNVMATEHQQQKEEKRPGSGRSFQFDKFNQSQQNYESSSYVNHQQPPQTQPQQMQQQSNTNTNNSKFMSFFANEGNSSSSSLNEFFKQAINQGHGNNPEQPKSLGHIGQMPSVDQLEAKWRRNSLNNVGETANKQTDNFQKLIGSLSSAKPQSQAVGYDAISNFIMQQQQYQQQQQKQHLIIQQQQQHTAFLASLQLKAILGRADTQLLLLRLTKGEISKHGLLVQLANPRLTDMDREAITAVLQFTNTQQQQQQHKQQLDMLSSTVIASQLQNLHNLAIVQQTLAARQQPQHNPQTQAPHQLSQEDLQAHANVIMRNAVMKRKIEEQTSKLINGGAKHQAQQQYLNRGQQRQARPDANSNALLHALISGGGNNHASGYPMNGQPQKHHSNLRFGDNQNFQSFESNQPHFATQYKQQYQQSQQQHPHQQPQQLNSLHQNNAGAVNSFNKAQMQAQSAISMLPNSGDEFH smg_drome Protein Smaug smg CG5263 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKYATGTDNAMTSGISGQTNNSNSASNEMQPTTSTPTAAHKEATSTATTTATYANGNPNSNANPSQSQPSNALFCEQVTTVTNLFEKWNDCERTVVMYALLKRLRYPSLKFLQYSIDSNLTQNLGTSQTNLSSVVIDINANNPVYLQNLLNAYKTFQPCDLLDAMSSSSSDKDSMPCYGSDFQITTSAQCDERKLYARKEDILHEVLNMLPLLKPGNEEAKLIYLTLIPVAVKDTMQQIVPTELVQQIFSYLLIHPAITSEDRRSLNIWLRHLEDHIQAAAAGLTNRSYFLQPSPQLVAGGSSTGSGSCSSSATSSSTASCSSVASSSLCPASGSRSSRTNDWQTIAPPSKQLQNKLAGDWRGNGGGSSSGSINPLCDNLNGITLNELASSQNSLGLSLEGSSSLVNGVVAGAGSMLGIAGGDDHDTSFSKNGTEILDFDPVTADMGEACSLASSSLCGRNGGNPVEDRSQPPPNLQQQLLQPPPYASILMGNVGDQFGEINRWSLDSKIAALKTRRSNSLTTQTISSCSSSSNSSVITVNDNCSNSTENLAQFANKPRSFSLSIEHQRGALMNSGSDTRLDEFKPNYIKFHTRNVGMSGIGLWLKSLRLHKYIELFKNMTYEEMLLITEDFLQSVGVTKGASHKLALCIDKLKERANILNRVEQELLSGQMELSTAVEELTNIVLTPMKPLESPGPPEENIGLRFLKVIDIVTNTLQQDPYAVQDDETLGVLMWILDRSIHNEAFMNHASQLKDLKFKLSKMKISMVPKMHHVKPAGVGPNNGNINKPRWNGKTRKCDTKNGSNDRINNRKNSNDMLNFSLNCLPHPLPHHSQQAPPPLPQFDYNGYGGGPSHQPQYKSSSYPSFMGNPQQQPPPPPSSKSHHHPQQMQQMLQQHNHFPALPQQTPPQSHRRSLNNLILVAGGPQQPQQLIFKPGQGVLTNNGSNDNLVLERNQQSQQQQQQRKLSGGVSSAEQQPKKTMAAVVMENLAKFDQHFTLF if4e_drome Eukaryotic translation initiation factor 4E1 eIF4E1 Eif4e eIF-4E eIF-4F 25 kDa subunit mRNA cap-binding protein CG4035 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSDFHRMKNFANPKSMFKTSAPSTEQGRPEPPTSAAAPAEAKDVKPKEDPQETGEPAGNTATTTAPAGDDAVRTEHLYKHPLMNVWTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKDTMVKQGSNVKSIYTL o61380_drome eIF4G1 eIF-4G eIF4G eIF4G-RA Dmel\CG10811 EIF4G Eif4G deIF4G eIF4g p200 Dmel_CG10811 CG10811 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQQAIPTLPTQSDIDKAMQPHSAQNMYVSTGNNNSGNTRSNPQSGGIFRGPPSTPNAPRGASGGATRHVHVQPMYSQPLHQNMVLQQYTQYNPRQQTFPASHLQYAPAPMPYYQYQYVPTLQQQPPHTRSAVTVNTNVNVGNNLQPVQSGPNGPLPVPGASSSQIQLLTSTVQPGASTVMGVGGPGSTMGQVGVPPMVGVGVMTTSVQPQPVQVQPASRRRHQHRLQIIDPTTKKNILDDFDKTKSNTDNEFSDQVTSTNTPATVLSEGPIRIPQQESVGLNNLTSTSSQGSESRTNAPYIPIETTPISRTDVGPTPIVSAMTDAPSVEILPTPQRGRSKKIPIVSPKNVSESIVAPITDETDDAGSTPISRATEESMPPNQTHPNLLISDSSKHKQAVSNSEISKDAPTGLKEMHVAELESSVASENHPAGILDVNVNKSSQSLDFSADPEIDSIGAISPTSPNAVSPILHEVLDNTELSKKLENSTTERFKDSQSVEKPTHQELSLRNATDETEISAMALQDVNSLDNNQIEKTYPSKPKLNVDVSEDISSRESAIKSTSTKNTGVDVGLQSDSKPETTLNDKQDSTDLKVKVSAKISSIINYNEGQWSPNNPSGKKQYDREQLLQLREVKASRIQPEVKNVSILPQPNLMPSFIRNNNNNKRVQSMVGIIGNRSNESAGNYIGKQMSMSGVQSGGGRSSMKGMIHVNLSLNQDVKLSENENAWRPRVLNKSDGDSDAKSALEKDELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEFTQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLLNPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKWQTSRNEAPKTMGQIEKEAKNEQLSAQYFGTLSSTTPGGSQGGSGKRDDRGNSRYGESRSSSAYGGSHSQRGDNGNLRHQQQNNVGGNVSGGAGHSNGNNDENTWHVQTSKGSRSLAVDSNKLEGLSKLSDQNLETKKMGGLTQFIWISSDTTRLSSAPTPTPSNPFAVLSSLIDKNSNERDRDRSGPRNKGSYNKGSMERDRYDRGMHSRTGSSQGSRENSSSRGGQQGRTLLSSSVQKSTSHSKYTQQAPPTRHTVKAQSSVGSSNVNTGPLYRGSEQQTSATFSQTTRSVAPVAVFIEASETDLKLIKSVVSEIVDLSAASKEVTPGAVSCIKRVPEKLRCSFIYYILTDYLHLANVGKQYRRYLSIAVSQLIQQNYISADHLRLAYNEFTVYANDLIVDIPELWLYILQFAGPLIVKKILTISDLWNNNLKENSPSNVAKKFLKTYLIYCTQEVGPNFARNMWIKFNLKWSDFMPESEVADFIKFNRLEYVENESKSPVIDHRETPEKHVKNVIDHIEHLLKEGTTADCIIDYSNGNIMVVDKLFIRGLTETLSNFSIHYKDNSYKLESETFQKFCIPVLQRYIDSNEDHQLECLYTLQLLVHGLEHPRGLLSELIGELYDAFVIQKESLCKWRDSKDQSAGKGVAVKSLNPFFNSLLNDDAN 7-methylguanosine 5'-diphosphate(1+) 7-methylguanosine 5'-diphosphate(1+) 7-methyl-GDP 7-methyl-GDP(1+) 7-methylguanosine diphosphate 7-methylguanosine diphosphate(1+) 7-methylguanosine 5'-(trihydrogen diphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1S/C11H17N5O11P2/c1-15-3-16(8-5(15)9(19)14-11(12)13-8)10-7(18)6(17)4(26-10)2-25-29(23,24)27-28(20,21)22/h3-4,6-7,10,17-18H,2H2,1H3,(H5-,12,13,14,19,20,21,22,23,24)/p+1/t4-,6-,7-,10-/m1/s1 SBASPRRECYVBRF-KQYNXXCUSA-O C[n+]1cn([C@@H]2O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]2O)c2nc(N)[nH]c(=O)c12 7-methyl-gtp 7-methyl-GTP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) smg-cup-1 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97707 n/a 97713 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97714 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cup binding sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig. 1b cup-eif4e Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97707 n/a 97715 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P48598:p.Glu106Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes N/A 97713 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false eif4e binding sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false eif4e binding sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9VMA3:p.Tyr327Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q9VMA3:p.[Leu364Ala;Leu368Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2 cup-smg Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97707 n/a 97715 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97713 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9VMA3:p.[Tyr327Ala;Leu364Ala;Leu368Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97714 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 583-763 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4 eif4e-cup Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97707 N/A 97715 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 97713 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1b eif_4e-cup Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97708 n/a 97716 unspecified role unspecified role prey prey 434-804 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97713 competitor competitor competitor prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 97715 unspecified role unspecified role bait bait cnbr-activated sepharose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 6 eif_4e-cup-1 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97708 n/a 97716 competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 97716 unspecified role unspecified role prey prey 434-804 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97715 unspecified role unspecified role bait bait cnbr-activated sepharose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 6 eif_4e-cup-2 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97708 n/a 97715 unspecified role unspecified role bait bait cnbr-activated sepharose tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 97716 unspecified role unspecified role prey prey 434-804 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false Fig. 6 7_methyl_gtp-cup Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97709 n/a 97717 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) n/a 97715 unspecified role unspecified role prey prey n/a 97713 unspecified role unspecified role prey prey Q9VMA3:p.[Leu364Ala;Leu368Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9VMA3:p.Tyr327Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 97718 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 3a 7_methyl_gtp-cup-1 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97709 n/a 97718 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) n/a 97713 unspecified role unspecified role prey prey Q9VMA3:p.[Leu364Ala;Leu368Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9VMA3:p.Tyr327Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 97715 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3a smg-cup-2 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97710 n/a 97714 unspecified role unspecified role bait bait n/a 97713 unspecified role unspecified role prey prey n/a 97715 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4 The addition of RNAse reduced the interaction of smaug with cup and eIF4E, respectively. cup-eif_4e-2 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97710 n/a 97715 unspecified role unspecified role prey prey n/a 97713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4 The addition of RNAse reduced the interaction of smaug with cup and eIF4E, respectively. smg-cup Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97711 N/A 97714 weight silver stain molecular weight estimation by silver staining weight silver stain ms participant Identification by mass spectrometry ms participant unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cup binding sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 97713 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1a cup-eif_4e-1 Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression 97712 n/a 97713 unspecified role unspecified role bait bait Q9VMA3:p.Tyr327Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9VMA3:p.[Tyr327Ala;Leu364Ala;Leu368Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 97715 unspecified role unspecified role prey prey radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P48598:p.Glu106Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false Fig. 3b