IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kroczynska-2004-1 The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid western blot western blot Immuno blot Immuno blot Kroczynska B., Evangelista CM., Samant SS., Elguindi EC., Blond SY. J. Biol. Chem. (0021-9258) 2004 kroczynska-2004-2 The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kroczynska B., Evangelista CM., Samant SS., Elguindi EC., Blond SY. J. Biol. Chem. (0021-9258) 2004 kroczynska-2004-3 The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom monoclonal western monoclonal antibody western blot monoclonal western Kroczynska B., Evangelista CM., Samant SS., Elguindi EC., Blond SY. J. Biol. Chem. (0021-9258) 2004 kroczynska-2004-4 The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity. in vitro In vitro biochemical biochemical monoclonal western monoclonal antibody western blot monoclonal western Kroczynska B., Evangelista CM., Samant SS., Elguindi EC., Blond SY. J. Biol. Chem. (0021-9258) 2004 mt1h_human Metallothionein-1H MT1H Metallothionein-IH Metallothionein-0 protein protein human Homo sapiens Human MDPNCSCEAGGSCACAGSCKCKKCKCTSCKKSCCSCCPLGCAKCAQGCICKGASEKCSCCA dnjc1_human DnaJ homolog subfamily C member 1 DNAJC1 DnaJ protein homolog MTJ1 HTJ1 protein protein human Homo sapiens Human MTAPCSQPAQLPGRRQLGLVPFPPPPPRTPLLWLLLLLLAAVAPARGWESGDLELFDLVEEVQLNFYQFLGVQQDASSADIRKAYRKLSLTLHPDKNKDENAETQFRQLVAIYEVLKDDERRQRYDDILINGLPDWRQPVFYYRRVRKMSNAELALLLFIILTVGHYAVVWSIYLEKQLDELLSRKKREKKKKTGSKSVDVSKLGASEKNERLLMKPQWHDLLPCKLGIWFCLTLKALPHLIQDAGQFYAKYKETRLKEKEDALTRTELETLQKQKKVKKPKPEFPVYTPLETTYIQSYDHGTSIEEIEEQMDDWLENRNRTQKKQAPEWTEEDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVTDVTTKAKQLKDSVTCSPGMVRLSELKSTVQNSRPIKTATTLPDDMITQREDAEGVAAEEEQEGDSGEQETGATDARPRRRKPARLLEATAKPEPEEKSRAKRQKDFDIAEQNESSDEESLRKERARSAEEPWTQNQQKLLELALQQYPRGSSDRWDKIARCVPSKSKEDCIARYKLLVELVQKKKQAKS aact_human Alpha-1-antichymotrypsin Cell growth-inhibiting gene 24/25 protein SERPINA3 AACT GIG24 GIG25 Serpin A3 protein protein human Homo sapiens Human MERMLPLLALGLLAAGFCPAVLCHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA dnajc1-mt1h-1 Interaction detected by Y2H. 101783 N/A 101787 unspecified role unspecified role prey prey N/A 101788 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false dnajc1-act-1 ACT interacts with the SANT2 domain of HTJ1 detected by Y2H 101784 N/A 101788 unspecified role unspecified role bait bait N/A 101789 unspecified role unspecified role prey prey physical association physical association false false false SANT2 domain (AA 493-554) of Q96KC8 found necessary for interaction. AA520 and AA554 in Q96KC8 found important for successful interaction. AA 140-343 of Q9UNU9 used in interaction. dnajc1-act-2 HTJ1-ACT interaction in vitro 101785 N/A 101788 unspecified role unspecified role bait bait N/A 101789 unspecified role unspecified role prey prey physical association physical association false false false Interaction detected by monoclonal antibody to Q9UNU9 AA 493-554 of Q96KC8 and AA1-400 of Q9UNU9 used for interaction. AA497 and AA520 of Q96KC8 important for successful interaction. dnajc1-act-3 Interaction of the SANT2 domain of HTJ-1 with human plasma glycosylated ACT. 101786 N/A 101788 unspecified role unspecified role neutral component neutral component N/A 101789 unspecified role unspecified role neutral component neutral component physical association physical association false false false The SANT2 domain (AA 493-554)of Q96KC8 used for interaction. AA 497 and 520 of Q96KC8 were found to be important for the interaction. Interaction not SDS-resistant. Monoclonal antibody for Q9UNU9 used for detection.