IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ derenzo-2003-1 Exclusion of germ plasm proteins from somatic lineages by cullin-dependent degradation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence DeRenzo C., Reese KJ., Seydoux G. Nature (0028-0836) 2003 derenzo-2003-2 Exclusion of germ plasm proteins from somatic lineages by cullin-dependent degradation. in vitro In vitro pull down pull down predetermined predetermined participant predetermined DeRenzo C., Reese KJ., Seydoux G. Nature (0028-0836) 2003 zif1_caeel Zinc finger-interacting factor 1 F59B2.6 zif-1 tag-6 protein protein caeel Caenorhabditis elegans MSECSASTSQLSTFCVNEIPWKLVPQTCDEFSEYEFIDEKYRRLSDVESFVWKWSPCGRYGIDLPGGRDVINQQLRDRMAVFDLHTNKWTLYTMDRGAFEIPRVCFLYWARNDIIGTITLVKDYEAGPDDEIPLKFKQSFFYVSHETQTLVYSGSCKYRTNKTIVASYWYHIFENRRGEPFIDHNNYLWLVFSNGVDSLILLPFIPTSTCFKAKCVSFDMNDIVSQFTGIRVFLDPFVSSHYVPWVYRNDVNFFIRIDSHSFIEEQNRDYGLDPQIDNPETKPHRGVFQLRVNLKDVIEKGEKDAIHAVNPETIETRFNRGDIELWRKISDELRQEHQSIAQHGKMVVVQRWSHFTVDMGLSKKMRTRVADLDARDYCCSHKPRNSSFCCIDLDSERIKAVDALILGTDVVLPHPSGSVFLFRYKENYGMSYCELPFFQPLSLKLKCIQVLKESVEIDPSYYRIKCCLKMQEELEKETAENLCTINIQQ pie1_caeel Pharynx and intestine in excess protein 1 pie-1 pic-1 Y49E10.14 protein protein caeel Caenorhabditis elegans MAQTKPIAEQMAALNNSDDTSFAADRSNSLLNATCPARIQNSVDQRKINRSFNDSLSSGYSGKWLRPKREALKITPLAQIDEAPATKRHSSAKDKHTEYKTRLCDAFRREGYCPYNDNCTYAHGQDELRVPRRRQEYYSRDPPRERRDSRSRRDDVDTTINRSSSSASKHHDENRRPSNNHGSSNRRQICHNFERGNCRYGPRCRFIHVEQMQHFNANATVYAPPSSDCPPPIAYYHHHPQHQQQFLPFPMPYFLAPPPQAQQGAPFPVQYIPQQHDLMNSQPMYAPMAPTYYYQPINSNGMPMMDVTIDPNATGGAFEVFPDGFFSQPPPTIIS g5ef15_caeel pos-1 F52E1.1 CELE_F52E1.1 protein protein caeel Caenorhabditis elegans MADNDFLSGEAIMVFKKEILDSHSDFTRSLSHQSASPEAYDQENVFSQDFQPFMKQDKETQNSASQPTSEQSLANRDPCTVPDDLREEMMRQRRKEDAFKTALCDAYKRSQACSYGDQCRFAHGVHELRLPMNPRGRNHPKYKTVLCDKFSMTGNCKYGTRCQFIHKIVDGNAAKLASGAHANTSSKSPARNAAAHNHSLFVPQGSTDRSMDLNQSLPIRQSDLVRAFARATRLDVSGYNSTAQLAQYFESQFQRIQQLSSHHH g5ecb9_caeel mex-1 W03C9.7 CELE_W03C9.7 protein protein caeel Caenorhabditis elegans MQSSNGEHHQQKQTPYFMNAPCDPALLALAQQTFLGFQPQKNMYQHGFPPMLPHRNLIPSLAAAIPEMDKLMLEEPIEAGSMPYRRATNKNISVSDDSFNNMHRSSSSSQYRRHSAQWETMTDDERESVQRQKRKEEAFKTALCDAFKRSGSCPYGEACRFAHGENELRMPSQPRGKAHPKYKTQLCDKFSNFGQCPYGPRCQFIHKLKKGLPLSEYNRALQEGEISPARDDEITNPDESSSQVEDLSELHHRQHQQQQQYQQRYRRPPFNNFHDMSDSGYSAPRRRLHHQFEHLGSEQQQQQQQQTPAPKIVYPSMQVVNIEMATGDGPKRSDNHHYHHHHHQRRHIPAPPSTGPLSSLIDQADYEASMASGKMFGKPENIRTYGAMIRANEEHVERNQTGVVKKERATVLPKRDLSLIREEDTLLVDGSHLNMTSSAILPAHQIPSTTTTSTSSPVQEQPWYEKIFGKMTMIQEESSMGGEDDDAHEDHYSR mex5_caeel Zinc finger protein mex-5 W02A2.7 mex-5 protein protein caeel Caenorhabditis elegans MKAASNSVSSAGGSVSPTTTQPPLPPGQSSHPQIYDQQMQYYFAAAMPNQPMATYAAQNGSSQQYAPAAPYYQDANGQYVQVPANGSMAPQQHMMVSGQPYLYMAQPQQGAQQVMQSGQPQLIYYQQSMAPQAAPMYFHPMQAAPMLPEQMGVMPHTQPAIPPQQQPRQVGVEISSTRTAPLTSSTPLPTSLEYETVQRDNRNRNIQFRYHRVMEHDELPIDEISKITLDNHNDDTMSAEKENHFHEHRGEKFGRRGFPIPETDSQQPPNYKTRLCMMHASGIKPCDMGARCKFAHGLKELRATDAPARYPNNKYKTKLCKNFARGGTGFCPYGLRCEFVHPTDKEFQNIPPYQRMSHDDQDYDQDVIPEDYVVARHQPRFMRTGGRATTPTKVMLKHRNVAGSMMCLSNAGRDLQAGGDYNQPESNEDDLPPHLRRNRRENPPMNKRRTSLSTKWTSEENLGLRGHY mex6_caeel Zinc finger protein mex-6 mex-6 AH6.5 protein protein caeel Caenorhabditis elegans MTATSNSAPLAGGSLSSSATAQPPQPPPGHQQQHPLPQIYDSQMQYYYGSSMPNQPIPTYTAQNGAPQQFGTPPYYQDANGQFGQVPAQQQMMTAGHPYFYMAQPQQGGQHVAQSGQPQIFYYQQPLGQMAQQAAPMYFHPMQAASTPMLSEQMSMMPQIQSTNPQQSEQLRKSGAQISTTRTVPLTSSTPLPTSREYETVQRDRNRNSQSRYQCPIEHDDLPIDEISKITIDNHNDDTMSAEKENRFNKNRVEKLGRRGFAKPEVDSQLPHNFKTRLCMTHAAGINPCALGARCKFAHGLKELRASDIPTRYPNNKYKTKLCKNFARGGSGVCPYGLRCEFVHPSDTEFQNIPPYQRKMVEEHDSIPEDYVVARYQPRFMHTSGKATTPTKVTLKQRNVAGSMMCLSNTGRDLEAGGDFNHPEINENDLPPHLRRIRRGNPPVTRSRPSFSTKWTSVENLGLRGHY q9bks1_caeel Elongin-C elc-1 Y82E9BR.15 CELE_Y82E9BR.15 protein protein caeel Caenorhabditis elegans MADQNNAIQCDQDAAQPKQYGGIEGPTSQYVKLVSSDDHEFIIKRELALTSGTIRAMLSGPGVYAENESNVVYFREIPSHVLQKVCQYFAYKVRYTHAATEIPEFPIPPDVALELLMAANFLDC zif1-pie1 Interaction of C.elegans zif-1 and pie-1 demonstrated by Y2H 103375 N/A 103377 unspecified role unspecified role bait bait N/A 103378 unspecified role unspecified role prey prey physical association physical association false false false Interaction requires at least one zinc finger domain (IPR000571) to be present in pie-1 zif1-pos1 Interaction of C.elegans zif-1 and pos-1 demonstrated by Y2H 103375 N/A 103377 unspecified role unspecified role bait bait N/A 103379 unspecified role unspecified role prey prey physical association physical association false false false Interaction requires at least one of the zinc fingers (IPR000571) to be present in pos-1 zif1-mex1 Interaction of C.elegans zif-1 and mex-1 demonstrated by Y2H 103375 N/A 103377 unspecified role unspecified role bait bait N/A 103380 unspecified role unspecified role prey prey physical association physical association false false false Interaction requires at least one zinc finger (IPR000571) to be present in mex-1 zif1-mex5 Interaction between C.elegans zif-1 and mex-5 identified by Y2H 103375 N/A 103381 unspecified role unspecified role prey prey N/A 103377 unspecified role unspecified role bait bait physical association physical association false false false Interaction requires at least one zinc finger domain (IPR000571) to be present in mex-5 zif1-mex6 Interaction between C.elegans zif-1 and mex-6 identified by Y2H 103375 N/A 103377 unspecified role unspecified role bait bait N/A 103382 unspecified role unspecified role prey prey physical association physical association false false false Interaction requires at least one zinc finger (IPR000571) to be present in mex-6 zif1-elc1 Interaction between C.elegans zif-1 and elc-1 identified by Y2H 103375 N/A 103377 unspecified role unspecified role bait bait N/A 103383 unspecified role unspecified role prey prey physical association physical association false false false Interaction requires Cys-447 to be present in zif-1 pie1-zif1 Interaction between C.elegans zif-1 and pie-1 confirmed by co-immunoprecipitation 103376 N/A 103377 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 103378 unspecified role unspecified role prey prey region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false Deletion mutant showed first Zinc-finger (IPR000571) of pie-1 to be required for interaction