IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ salvaing-2003-2 The Drosophila Corto protein interacts with Polycomb-group proteins and the GAGA factor. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Salvaing J., Lopez A., Boivin A., Deutsch JS., Peronnet F. Chromatin - Epigenetic interactions resulting in chromatin modulation heat shocked embroys used Nucleic Acids Res. (0305-1048) 2003 salvaing-2003-3 The Drosophila Corto protein interacts with Polycomb-group proteins and the GAGA factor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Salvaing J., Lopez A., Boivin A., Deutsch JS., Peronnet F. Chromatin - Epigenetic interactions resulting in chromatin modulation heat shocked embroys used Nucleic Acids Res. (0305-1048) 2003 salvaing-2003-1 The Drosophila Corto protein interacts with Polycomb-group proteins and the GAGA factor. drome Drosophila melanogaster (Fruit fly) Fruit fly coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Salvaing J., Lopez A., Boivin A., Deutsch JS., Peronnet F. Chromatin - Epigenetic interactions resulting in chromatin modulation heat shocked embroys used Nucleic Acids Res. (0305-1048) 2003 salvaing-2003-4 The Drosophila Corto protein interacts with Polycomb-group proteins and the GAGA factor. drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland imaging technique imaging technique microscopy immunostaining immunostaining Salvaing J., Lopez A., Boivin A., Deutsch JS., Peronnet F. Chromatin - Epigenetic interactions resulting in chromatin modulation heat shocked embroys used Nucleic Acids Res. (0305-1048) 2003 gaga_drome Transcription factor GAGA Trl GAGA TFGAGA Trithorax-like protein GAGA factor Adh transcription factor 2 Neural conserved at 70F Adf-2 Nc70F CG33261 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKTTSGKKSSSGSSGSGSGALSSSGSVPQVQTVQSLHTLQGVQVKKDPDAQQQQQQQQQQQQQQQQAMTVSGATGGQVQQQVQQVQQQVQQQQQQQQQQQQQLQHHQIIDSSGNITTATTSAQAAAAAQQQAAGQQQQLVAQSDGSESGAPLSIAQVQTLQGHQIIGNLNQVNMTDFQQQQPQQQQQQQQQQQQQQQQQQQTQQTL corto_drome Centrosomal and chromosomal factor corto ccf CG2530 Chromocentrosomin protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTMAACYANYDMSSLSHGMSALSALQQQQQQQQQQHSQTQQQHHHQQQQQHMYHAAVAAHQQQLLQQQQQQQHHRHHHQPANTSSSSNSRHSHAAATQTQVAAAVANSRQQQQQQQQQQQQQQQQQTASSNSNAAPAPSPQKDYSIPLHVDCSVEYELPNQPKPPAGQRVEPLLMIHPCYFRKMESQRRSPFVNNMHATARAVSSSSLSSGAALGGGGSGAAVATAPSSSAARRGARAATSAQQQQQQQQRYQQQQQQLRQQHQQMSQMSQQAHYPQQQSSLVRQHQQQQQQQQQQQRASSNQSQRQSQSQSQSQSHAANSAAAAAAQASIAAAAAGQWDQLAALAARTALTPHHMLHPHSHYAAKGSGGGAGGGKRDAMISGSYGQTAVASGKLQQSQVQQQQPQQQQQHCLPPPPWDATSMLMDRSPMATVPSNYQAGPDTNPMRLYSATPTAGAASGGSASVGGGGAVSGAGATGAVNTATDKLSGKYRQYLRSQRMHPYAAAASLNLAAAAAAAGQTSFVPFSSAATAVAATPTFQHLPQISCYNV esc_drome Polycomb protein esc esc CG14941 Protein extra sex combs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSDKVKNGNEPEESEESCGDESASYTTNSTTSRSKSPSSSTRSKRRGRRSTKSKPKSRAAYKYDTHVKENHGANIFGVAFNTLLGKDEPQVFATAGSNRVTVYECPRQGGMQLLHCYADPDPDEVFYTCAWSYDLKTSSPLLAAAGYRGVIRVIDVEQNEAVGNYIGHGQAINELKFHPHKLQLLLSGSKDHAIRLWNIQSHVCIAILGGVEGHRDEVLSIDFNMRGDRIVSSGMDHSLKLWCLNTPEFHHKIELSNTFSQEKSTLPFPTVTKHFPDFSTRDIHRNYVDCVQWFGNFVLSKSCENAIVCWKPGQLHQSFEQVKPSDSSCTIIAEFEYDECEIWFVRFGFNPWQKVIALGNQQGKVYVWELDPSDPEGAHMTTLHNSRSVATVRQIAFSRDASVLVYVCDDATVWRWNRRQTTSI scm_drome Polycomb protein Scm Scm CG9495 Sex comb on midleg protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGGRDSSTSSGSNSAAPGASTNATSSASASASSTSTSASPGSTTSPASTQRQRGRPAKRATCTWCGEGKLPLQYVLPTQTGKKEFCSETCIAEFRKAYSKGACTQCDNVIRDGAPNKEFCSIMCMNKHQKKNCSTRHSGGSASGKGLAESERKLLASGAPAPTGPFQYESFHVFDWDAYLEETGSEAAPAKCFKQAQNPPNNDFKIGMKLEALDPRNVTSTCIATVVGVLGSRLRLRLDGSDSQNDFWRLVDSTEIHAIGHCEKNGGMLQPPLGFRMNASSWPGYLCKILNNAMVAPEEIFQPEPPEPEENLFKVGQKLEAVDKKNPQLICCATVDAIKDDQIHVTFDGWRGAFDYWCNYRSRDIFPAGWCARSCHPMQPPGHKSRMDSSSSKQRCPRPRYTVVAESEAMVPASPATAHFHPNCKGGPFINNSKLPCMVTGPTYQTLAKLCLQEVLAASTDTQQLSKLLFALEGDVHIVTAAGKNFTVKIPSPMRMKDDESLAQFIETLCTTCRACANLISLVHETEECKKCANSRKRQLTQSATPPSSPVLADKRNRQSNSATTSPSEKIIKQELAVKSPVESKSKTSTNNGKEPASQQNSNHSLNNNNNSASKSSNKVVIKSEPNGANAQTSSTTQALRKVRFQHHANTNTNSSATNGNQDTSQTTHVSTSHCSSSSTSSSTSLAGGSANTSTIGKYLAPLVAEVHPEQANVKPSNSYYKSPTTLSSSASLPTSVSTPFTGCQSASSTALAAGGVTAAKAATAPAGAAATAGASPSYTAITSPVSTPTSALANSHLRSQPIDWTIEEVIQYIESNDNSLAVHGDLFRKHEIDGKALLLLNSEMMMKYMGLKLGPALKICNLVNKVNGRRNNLAL ez_drome Histone-lysine N-methyltransferase E(z) KMT6 E(z) CG6502 Protein enhancer of zeste Lysine N-methyltransferase 6 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYNGIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIELTEHQDPERPQECTPNIDGIKAESVSRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQKEDAEFSFEDLRQDFTPPRKKKKKQRLWSLHCRKIQLKKDSSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACGADQFKLTKITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREMEIV php_drome Polyhomeotic-proximal chromatin protein CG18412 ph-p protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDRRALKFMQKRADTESDTTTPVSTTASQGISASAILAGGTLPLKDNSNIREKPLHHNYNHNNNNSSQHSHSHQQQQQQQVGGKQLERPLKCLETLAQKAGITFDEKYDVASPPHPGIAQQQATSGTGPATGSGSVTPTSHRHGTPPTGRRQTHTPSTPNRPSAPSTPNTNCNSIARHTSLTLEKAQNPGQQVAATTTVPLQISPEQLQQFYASNPYAIQVKQEFPTHTTSGSGTELKHATNIMEVQQQLQLQQQLSEANGGGAASAGAGGAASPANSQQSQQQQHSTAISTMSPMQLAAATGGVGGDWTQGRTVQLMQPSTSFLYPQMIVSGNLLHPGGLGQQPIQVITAGKPFQGNGPQMLTTTTQNAKQMIGGQAGFAGGNYATCIPTNHNQSPQTVLFSPMNVISPQQQQNLLQSMAAAAQQQQLTQQQQQFNQQQQQQLTQQQQQLTAALAKVGVDAQGKLAQKVVQKVTTTSSAVQAATGPGSTGSTQTQQVQQVQQQQQQTTQTTQQCVQVSQSTLPVGVGGQSVQTAQLLNAGQAQQMQIPWFLQNAAGLQPFGPNQIILRNQPDGTQGMFIQQQPATQTLQTQQNQIIQCNVTQTPTKARTQLDALAPKQQQQQQQVGTTNQTQQQQLAVATAQLQQQQQQLTAAALQRPGAPVMPHNGTQVRPASSVSTQTAQNQSLLKAKMRNKQQPVRPALATLKTEIGQVAGQNKVVGHLTTVQQQQQATNLQQVVNAAGNKMVVMSTTGTPITLQNGQTLHAATAAGVDKQQQQLQLFQKQQILQQQQMLQQQIAAIQMQQQQAAVQAQQQQQQQVSQQQQVNAQQQQAVAQQQQAVAQAQQQQREQQQQVAQAQAQHQQALANATQQILQVAPNQFITSHQQQQQQQLHNQLIQQQLQQQAQAQVQAQVQAQAQQQQQQREQQQNIIQQIVVQQSGATSQQTSQQQQHHQSGQLQLSSVPFSVSSSTTPAGIATSSALQAALSASGAIFQTAKPGTCSSSSPTSSVVTITNQSSTPLVTSSTVASIQQAQTQSAQVHQHQQLISATIAGGTQQQPQGPPSLTPTTNPILAMTSMMNATVGHLSTAPPVTVSVTSTAVTSSPGQLVLLSTASSGGGGSIPATPTKETPSKGPTATLVPIGSPKTPVSGKDTCTTPKSSTPATVSASVEASSSTGEALSNGDASDRSSTPSKGATTPTSKQSNAAVQPPSSTTPNSVSGKEEPKLATCGSLTSATSTSTTTTITNGIGVARTTASTAVSTASTTTTSSGTFITSCTSTTTTTTSSISNGSKDLPKAMIKPNVLTHVIDGFIIQEANEPFPVTRQRYADKDVSDEPPKKKATMQEDIKLSGIASAPGSDMVACEQCGKMEHKAKLKRKRYCSPGCSRQAKNGIGGVGSGETNGLGTGGIVGVDAMALVDRLDEAMAEEKMQTEATPKLSESFPILGASTEVPPMSLPVQAAISAPSPLAMPLGSPLSVALPTLAPLSVVTSGAAPKSSEVNGTDRPPISSWSVDDVSNFIRELPGCQDYVDDFIQQEIDGQALLLLKEKHLVNAMGMKLGPALKIVAKVESIKEVPPPGEAKDPGAQ asx_drome Polycomb protein Asx Asx CG8787 Additional sex combs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGPNIAYIDGSNTNSSAVALMEAGNNYIVTTNASPTAAPSPINQQPQSQPTGTQHQHPLLQLHQTGENTPPGNEATATANNCACSLNAMVICQQCGAFCHDDCIGAAKLCVACVIR corto-trl-1 Interaction detected by GST pulldown 103287 N/A 103291 unspecified role unspecified role prey prey N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false AA 127-203 of corto was expressed with GST tag. corto-corto-1 Interaction detected by GST pulldown 103287 N/A 103292 unspecified role unspecified role prey prey N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false AA 127-203 of Corto was tagged to GST as a bait. corto-esc-2 Interaction detected by GST pulldown 103287 N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 103293 unspecified role unspecified role prey prey physical association physical association false false false AA 127-203 of corto was expressed in E. coli with a GST tag. corto-scm-1 Interaction detected by GST pulldown 103287 N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 103294 unspecified role unspecified role prey prey physical association physical association false false false AA 127-203 of corto was expressed in E coli with a GST tag. corto-e_z_-1 Interaction detected by GST pulldown 103287 N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 103295 unspecified role unspecified role prey prey physical association physical association false false false AA 127-203 of Corto tagged to GST was used as bait corto-ph_p-2 Interaction detected by GST pull down 103287 N/A 103292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 103296 unspecified role unspecified role prey prey physical association physical association false false false AA 127-203 of Corto tagged to GST was used as bait corto-corto-2 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role prey prey N/A 103292 unspecified role unspecified role bait bait physical association physical association false false false AA 127-203 of Corto was used as Lex-A fusion bait. corto-esc-3 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role bait bait N/A 103293 unspecified role unspecified role prey prey physical association physical association false false false AA 1-324 of Corto was used as bait. Full length ESC was used as prey. corto-e_z_-2 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role bait bait N/A 103295 unspecified role unspecified role prey prey physical association physical association false false false AA 1-315 of E(Z) interacts with AA 127-203 of Corto. corto-ph_p-1 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role bait bait N/A 103296 unspecified role unspecified role prey prey physical association physical association false false false Full length Corto was used as bait. AA 1-522 of PH was used as prey. corto-scm-2 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role bait bait N/A 103294 unspecified role unspecified role prey prey physical association physical association false false false AA 127-203 or AA 440-550 of Corto corto-trl-2 Interaction detected by Y2D 103288 N/A 103292 unspecified role unspecified role bait bait N/A 103291 unspecified role unspecified role prey prey physical association physical association false false false AA 1-324 of Corto was used as bait. Full length trl-GAGA was used as prey. corto-esc-1 Interaction detected by coIP 103289 N/A 103293 unspecified role unspecified role bait bait N/A 103292 unspecified role unspecified role prey prey physical association physical association false false false ESC was expressed as Lex-A fusion corto-asx-1 Interaction detected by coIP 103289 N/A 103292 unspecified role unspecified role prey prey N/A 103297 unspecified role unspecified role bait bait physical association physical association false false false PC was expressed as Lex-A fusion corto-e_z_-3 Immunolocalization 103290 N/A 103292 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103296 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103291 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103294 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103297 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103295 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland N/A 103293 unspecified role unspecified role neutral component neutral component drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland colocalization colocalization false false false