IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ girrbach-2003-1 Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves. yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Girrbach V., Strahl S. J. Biol. Chem. (0021-9258) 2003 imex curation Only protein-protein interactions girrbach-2003-2 Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves. yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Girrbach V., Strahl S. J. Biol. Chem. (0021-9258) 2003 imex curation Only protein-protein interactions pmt2_yeast Dolichyl-phosphate-mannose--protein mannosyltransferase 2 PMT2 FUN25 YAL023C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERDAEDFSKEKPAAQSSLLRLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKMLVGLSGYLAGYNGSWDFPSGEIYPDYLDYVKMRLFNASFSALCVPLAYFTAKAIGFSLPTVWLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFVMFHNQRSKPFSRKWWKWLLITGISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKSMSWKTYINHWLARIFGLIIVPFCIFLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSDVGQGPRDIALGSSVVSIKNQALGGSLLHSHIQTYPDGSNQQQVTCYGYKDANNEWFFNRERGLPSWSENETDIEYLKPGTSYRLVHKSTGRNLHTHPVAAPVSKTQWEVSGYGDNVVGDNKDNWVIEIMDQRGDEDPEKLHTLTTSFRIKNLEMGCYLAQTGNSLPEWGFRQQEVVCMKNPFKRDKRTWWNIETHENERLPPRPEDFQYPKTNFLKDFIHLNLAMMATNNALVPDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNPKYFLLGTPASTWASSVAVLAFMATVVILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWGLHYMPFVIMSRVTYVHHYLPALYFALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMALVIGCFWYFSPISFGMEGPSSNFRYLNWFSTWDIADKQEA pmt1_yeast Dolichyl-phosphate-mannose--protein mannosyltransferase 1 PMT1 D2390 YDL095W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEEKTYKRVEQDDPVPELDIKQGPVRPFIVTDPSAELASLRTMVTLKEKLLVACLAVFTAVIRLHGLAWPDSVVFDEVHFGGFASQYIRGTYFMDVHPPLAKMLYAGVASLGGFQGDFDFENIGDSFPSTTPYVLMRFFSASLGALTVILMYMTLRYSGVRMWVALMSAICFAVENSYVTISRYILLDAPLMFFIAAAVYSFKKYEMYPANSLNAYKSLLATGIALGMASSSKWVGLFTVTWVGLLCIWRLWFMIGDLTKSSKSIFKVAFAKLAFLLGVPFALYLVFFYIHFQSLTLDGDGASFFSPEFRSTLKNNKIPQNVVADVGIGSIISLRHLSTMGGYLHSHSHNYPAGSEQQQSTLYPHMDANNDWLLELYNAPGESLTTFQNLTDGTKVRLFHTVTRCRLHSHDHKPPVSESSDWQKEVSCYGYSGFDGDANDDWVVEIDKKNSAPGVAQERVIALDTKFRLRHAMTGCYLFSHEVKLPAWGFEQQEVTCASSGRHDLTLWYVENNSNPLLPEDTKRISYKPASFISKFIESHKKMWHINKNLVEPHVYESQPTSWPFLLRGISYWGENNRNVYLLGNAIVWWAVTAFIGIFGLIVITELFSWQLGKPILKDSKVVNFHVQVIHYLLGFAVHYAPSFLMQRQMFLHHYLPAYYFGILALGHALDIIVSYVFRSKRQMGYAVVITFLAASVYFFKSFSPIIYGTPWTQELCQKSQWLSGWDYNCNTYFSSLEEYKNQTLTKRESQPAATSTVEEITIEGDGPSYEDLMNEDGKKIFKDTEGNELDPEVVKKMLEEEGANILKVEKRAVLE pmt3_yeast Dolichyl-phosphate-mannose--protein mannosyltransferase 3 PMT3 O6148 YOR321W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPYRVATGYSEKSTDDDLIWRTPIVKEELEDADNFLKDDAELYDKVKNESAVSHLDTIVMPIIFTVLGMFTRMYKIGRNNHVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKMLVGLSGYLAGYNGSWDFPSGEVYPDYIDYVKMRLFQAMFSSLCVPLAYFTGRAIGFSRLSVWLFTILVIFENSYATLGKFILLDSMLLFFTVSSYFCLAKFHTMRKSPFSARWWLWLCLTGLNLGCAISVKMVGLFIISVVGIYTISELWNLLSDRSVSWKVYVNHWLARIFGLIIIPVCVFLLCFKIHFDLLSNSGPGDSTMPSLFQASLNGTKVGKGPRDVALGSSIISIKNQALGGALLHSHVQPFPEGSEQQQVTVYGYSDANNEWFFQRIRGVEPWTDAENKTIEFVKGGEMYRLMHRLTGKNLHTHEVPAPISKSEYEVSAYGDVDLGDYKDNWIIEIVEQVGEEDPTLLHPLSTSFRIKNSILGCYLAQSGKHLPEWGFRQGEVVCLKHASKRDKRTWWNIETHENERLPQGEDFVYPKTSFFRNFMQLNSAMMATNNALVPNPEKFDGIASSAWQWPTLNVGVRLCEWSEKSIKYFLLGSPASVWPSSIAVCALIIHVIFLTLKWQRQCVILSDPVERDVFVMAAFYPLLAWLLHYMPFVVMSRVVYAHHYLPTLYFALMILSYYFDMITKRWATRNTGKFLRLGAYIVYGSIVIAGFFYFSPFSFGMDGPVDDYAYLAWLPTWQIVEDIRNT F076473B41EB6CBA pmt5_yeast Dolichyl-phosphate-mannose--protein mannosyltransferase 5 PMT5 D2399 YDL093W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNKEHLLKVDPIPDVTIKRGPLRSFLITKPCDNLSSLRTVTSSKEKLLVGCLLIFTAIVRLHNISLPNSVVFGENEVGTFVSQYVNNIFFTDVHPPLVAMLYATVSSVFGYKGLFNYGNIGTEYTANVPYVAMRFFSATLGIVSVLVLYLTLRVSGVKIAVAAICAVCFAIENSFVTLSRFTLIEGPFVFFMACAVYFFRRSELYLPNSCKANKSLLAASIALGFAVSSKWAGLFTIAWAGIIVLWRVWFMIGDLSRPIGSSIKYMAFQFTCLLAIPAFIYFLIFSVHIKTLNVNGISSSFFPAEFRKTLKYNNVIKETVAEVAVGSAVSLNHVGTAGGYLHSHLHNYPAGSMQQQVTLYPHIDQNNKWIIELAEHPNENVTSFQNLTDGTIIKLRQLKNGCRLHSHDHKPPVSQNADWQKEVSCYGYEGFEGDINDDWIIEIDKKRSEPGPAQEHIRAIETKFRLKHYLTGCYLFSHPEKLPEWGFGQQEVTCAYFAREDLTSWYIEENENEISLPNPEKVSYKKMSFWQKFVAIHKFMFYLNNYMDTSHAYSSEPKTWPLMLRGIDFWNENGREVYFLGNAVLWWSVTAFICTFIIGVAVELLAWKLGVNILRDKHIINFHYQVFQYLLGFAAHYFPYFFVGQKLFLYDYLPAYYFGILAFGHALDLISTYISNKRNNTGYIVVAIFMVVCFYFFSEHSPLIYATGWSSNLCKRSKWLGSWDFYCNSLLLSDSHYELNAES E75625C236AC6A68 pmt4_yeast Dolichyl-phosphate-mannose--protein mannosyltransferase 4 PMT4 J2176 YJR143C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVPKKRNHGKLPPSTKDVDDPSLKYTKAAPKCEQVAEHWLLQPLPEPESRYSFWVTIVTLLAFAARFYKIWYPKEVVFDEVHFGKFASYYLERSYFFDVHPPFAKMMIAFIGWLCGYDGSFKFDEIGYSYETHPAPYIAYRSFNAILGTLTVPIMFNTLKELNFRAITCAFASLLVAIDTAHVTETRLILLDAILIISIAATMYCYVRFYKCQLRQPFTWSWYIWLHATGLSLSFVISTKYVGVMTYSAIGFAAVVNLWQLLDIKAGLSLRQFMRHFSKRLNGLVLIPFVIYLFWFWVHFTVLNTSGPGDAFMSAEFQETLKDSPLSVDSKTVNYFDIITIKHQDTDAFLHSHLARYPQRYEDGRISSAGQQVTGYTHPDFNNQWEVLPPHGSDVGKGQAVLLNQHIRLRHVATDTYLLAHDVASPFYPTNEEITTVTLEEGDGELYPETLFAFQPLKKSDEGHVLKSKTVSFRLFHVDTSVALWTHNDELLPDWGFQQQEINGNKKVIDPSNNWVVDEIVNLDEVRKVYIPKVVKPLPFLKKWIETQKSMFEHNNKLSSEHPFASEPYSWPGSLSGVSFWTNGDEKKQIYFIGNIIGWWFQVISLAVFVGIIVADLITRHRGYYALNKMTREKLYGPLMFFFVSWCCHYFPFFLMARQKFLHHYLPAHLIACLFSGALWEVIFSDCKSLDLEKDEDISGASYERNPKVYVKPYTVFLVCVSCAVAWFFVYFSPLVYGDVSLSPSEVVSREWFDIELNFSK pmt1-pmt2-1 Pmt1p-Pmt2p complex represent the predominant form. 165051 n/a 165053 unspecified role unspecified role prey prey n/a 165054 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P33775:p.Arg138Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P33775:p.Arg138Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2B, 6C Interaction observed in Pmt3-/- cells pmt1-pmt2-3 Pmt1p-Pmt2p complex represent the predominant form. 165051 n/a 165054 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 165053 unspecified role unspecified role prey prey n/a 165055 unspecified role unspecified role prey prey association association false false false Fig. 2B Interaction observed in Pmt3-/- cells pmt1-pmt3-1 Pmt1p-Pmt3p complex represent the compensatory form. 165051 n/a 165055 unspecified role unspecified role prey prey n/a 165054 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Interaction observed in Pmt2-/- cells Fig. 2B pmt1-pmt2-2 Pmt1p-Pmt2p complex represent the predominant form. 165051 n/a 165053 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 165054 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2C pmt2-pmt1 Pmt2p-Pmt5p complex represent the compensatory form. 165051 n/a 165054 unspecified role unspecified role prey prey n/a 165056 unspecified role unspecified role prey prey n/a 165053 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false In the absence of PMT3 (mutant strain) PMT5 will form a complex with PMT2. Fig. 2C pmt3-pmt1 Pmt3p-Pmt5p complex represent the predominant form. 165051 n/a 165054 unspecified role unspecified role prey prey n/a 165055 unspecified role unspecified role bait bait n/a 165056 unspecified role unspecified role prey prey association association false false false Fig. 2C pmt4-pmt4-1 Pmt4p acts as a homomeric complex 165051 n/a 165057 unspecified role unspecified role prey prey n/a 165057 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P46971:p.Arg142Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B pmt2-pmt5 Pmt2p-Pmt5p complex represent the compensatory form. 165052 n/a 165053 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 165056 unspecified role unspecified role neutral component neutral component physical association physical association false false false In the absence of PMT3 (mutant strain) PMT5 will form a complex with PMT2. Fig. 4B pmt4-pmt4-3 Pmt4p acts as a homomeric complex 165052 n/a 165057 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot c-term range C-terminal range c-term range c-term range C-terminal range c-term range false loop 5 deleted Loss of loop 5 (required for enzyme activity) does not affect dimerization binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 165057 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 5B, 6B