IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lambermon-2002-2 UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lambermon MH., Fu Y., Wieczorek Kirk DA., Dupasquier M., Filipowicz W., Lorkovic ZJ. Mol. Cell. Biol. (0270-7306) 2002 lambermon-2002-1 UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lambermon MH., Fu Y., Wieczorek Kirk DA., Dupasquier M., Filipowicz W., Lorkovic ZJ. Mol. Cell. Biol. (0270-7306) 2002 uba1a_arath UBP1-associated proteins 1A UBA1A UBP1-interacting protein 1a T16B14.6 At2g22090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKTLDKSKKRKLVKSKSTNFDKKQKINKQQQQPESSTPYSSSSSSSDSSDSESDNEFDPEELRELLQPYSKDQLVDLVCSASRIGSSIYSAVVEAADRDVTHRKIFVYGLPWETTRETLVGVFEGYGEIEECTVVIDKATGKAKGFGFVMFKTRKGAKEALKEPKKRILNRTATCQLASMGPAASGKGHDQPGPVKISMGSMANHGQPQQQQVQGQHVFNGGGMAASPFMLGNQYHPLYGAGMLGNPALAAAAAAGGGYMYPMLAGALAHGGLGSDMVQSSQMGGIGVDPSVGAAGLSALGSYFRGQSLPSTYPDSDAGGKRGTGKDSDAGGSSFHGYSNYS uba2a_arath UBP1-associated protein 2A UBA2A UBP1-interacting protein 2a T8M16.190 At3g56860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKRKLEGEESNEAEEPSQKLKQTPEEEQQLVIKNQDNQGDVEEVEYEEVEEEQEEEVEDDDDEDDGDENEDQTDGNRIEAAATSGSGNQEDDDDEPIQDLLEPFSKEQVLSLLKEAAEKHVDVANRIREVADEDPVHRKIFVHGLGWDTKTETLIEAFKQYGEIEDCKAVFDKISGKSKGYGFILYKSRSGARNALKQPQKKIGSRMTACQLASKGPVFGGAPIAAAAVSAPAQHSNSEHTQKKIYVSNVGAELDPQKLLMFFSKFGEIEEGPLGLDKYTGRPKGFCLFVYKSSESAKRALEEPHKTFEGHILHCQKAIDGPKPGKQQQHHHNPHAYNNPRYQRNDNNGYGPPGGHGHLMAGNPAGMGGPTAQVINPAIGQALTALLASQGAGLAFNPAIGQALLGSLGTAAGVNPGNGVGMPTGYGTQAMAPGTMPGYGTQPGLQGGYQTPQPGQGGTSRGQHGVGPYGTPYMGH ubp1a_arath Oligouridylate-binding protein 1A UBP1A Polyuridylate-binding protein UBP1A F15I1.16 At1g54080 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQNQRLIKQQQQQQQQQHQQAMIQQAMMQQHPSLYHPGVMAPPQMEPLPSGNLPPGFDPTTCRSVYAGNIHTQVTEILLQEIFASTGPIESCKLIRKDKSSYGFVHYFDRRCASMAIMTLNGRHIFGQPMKVNWAYATGQREDTSSHFNIFVGDLSPEVTDAALFDSFSAFNSCSDARVMWDQKTGRSRGFGFVSFRNQQDAQTAINEMNGKWVSSRQIRCNWATKGATFGEDKHSSDGKSVVELTNGSSEDGRELSNEDAPENNPQFTTVYVGNLSPEVTQLDLHRLFYTLGAGVIEEVRVQRDKGFGFVRYNTHDEAALAIQMGNAQPFLFSRQIRCSWGNKPTPSGTASNPLPPPAPASVPSLSAMDLLAYERQLALAKMHPQAQHSLRQAGLGVNVAGGTAAMYDGGYQNVAAAHQQLMYYQ ubp1b_arath Oligouridylate-binding protein 1B UBP1B Polyuridylate-binding protein UBP1B F1L3.2 F28G4.17 At1g17370 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQRLKQQQQQQQVMMQQALMQQQSLYHPGLLAPPQIEPIPSGNLPPGFDPSTCRSVYVGNIHIQVTEPLLQEVFAGTGPVESCKLIRKEKSSYGFVHYFDRRSAGLAILSLNGRHLFGQPIKVNWAYASGQREDTSSHFNIFVGDLSPEVTDAMLFTCFSVYPTCSDARVMWDQKTGRSRGFGFVSFRNQQDAQTAIDEITGKWLGSRQIRCNWATKGATSGEDKQSSDSKSVVELTSGVSEDGKDTTNGEAPENNAQYTTVYVGNLAPEVSQVDLHRHFHSLGAGVIEEVRVQRDKGFGFVRYSTHVEAALAIQMGNTHSYLSGRQMKCSWGSKPTPAGTASNPLPPPAPAPIPGFSASDLLAYERQLAMSKMAGMNPMMHHPQGQHAFKQAAMGATGSNQAIYDGGYQNAQQLMYYQ ubp1c_arath Oligouridylate-binding protein 1C UBP1C Polyuridylate-binding protein UBP1C MAG2.6 At3g14100 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQNPRLKQHQQQQQQQAMMQQQALMQQHSLYHPGVLAPPQLEPVPSGNLPPGFDPSTCRSVYVGNIHTQVTEPLLQEIFTSTGPVESSKLIRKDKSSYGFVHYFDRRSAALAILSLNGRHLFGQPIKVNWAYATGQREDTSSHFNIFVGDLSPEVTDATLYQSFSVFSSCSDARVMWDQKTGRSRGFGFVSFRNQQDAQTAINEMNGKWLSSRQIRCNWATKGATSGDDKLSSDGKSVVELTTGSSEDGKETLNEETPENNSQFTTVYVGNLAPEVTQLDLHRYFHALGAGVIEEVRVQRDKGFGFVRYNTHPEAALAIQMGNTQPYLFNRQIKCSWGNKPTPPGTASNPLPPPAPAPVPGLSAADLLNYERQLALSKMASVNALMHQQGQHPLRQAHGINAAGATAAMYDGGFQNVAAAQQLMYYQ uba1a-t8m16_190-3 Interaction detected by Y2H 121986 N/A 121988 unspecified role unspecified role bait bait N/A 121989 unspecified role unspecified role prey prey physical association physical association false false false ub11a-uba1a-1 Interaction detected by Y2H 121986 N/A 121988 unspecified role unspecified role bait bait N/A 121988 unspecified role unspecified role prey prey physical association physical association false false false uba1a-f15i1_16-1 Interaction detected by Y2H 121986 N/A 121988 unspecified role unspecified role bait bait N/A 121990 unspecified role unspecified role prey prey physical association physical association false false false uba1a-f1l3_2-2 Interaction detected by Y2H 121986 N/A 121988 unspecified role unspecified role bait bait N/A 121991 unspecified role unspecified role prey prey physical association physical association false false false uba1a-q9ljh8-1 Interaction detected by Y2H 121986 N/A 121988 unspecified role unspecified role bait bait N/A 121992 unspecified role unspecified role prey prey physical association physical association false false false t8m16_190-f15i1_16-1 Interaction detected by Y2H 121986 N/A 121989 unspecified role unspecified role bait bait N/A 121990 unspecified role unspecified role prey prey physical association physical association false false false t8m16_190-f1l3_2-1 Interaction detected by Y2H 121986 N/A 121989 unspecified role unspecified role bait bait N/A 121991 unspecified role unspecified role prey prey physical association physical association false false false t8m16_190-q9ljh8-1 Interaction detected by Y2H 121986 N/A 121989 unspecified role unspecified role bait bait N/A 121992 unspecified role unspecified role prey prey physical association physical association false false false uba1a-f1l3_2-1 Interaction detected by GST pull down 121987 N/A 121988 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 121991 unspecified role unspecified role prey prey nicpl Nicotiana plumbaginifolia (Leadwort-leaved tobacco) Tex-Mex tobacco physical association physical association false false false The protein were expressed with tags: GST-UBA1a and AtUBP1b-HA (hemagglutinin). t8m16_190-uba1a-1 Interaction detected by GST pull down 121987 N/A 121989 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 121988 unspecified role unspecified role prey prey nicpl Nicotiana plumbaginifolia (Leadwort-leaved tobacco) Tex-Mex tobacco physical association physical association false false false The protein were expressed with tags: GST-UBA2a and UBA1a-HA (hemagglutinin). uba1a-t8m16_190-2 Interaction detected by GST pull down 121987 N/A 121988 unspecified role unspecified role bait bait N/A 121989 unspecified role unspecified role prey prey physical association physical association false false false The protein were expressed with tags: GST-UBA1a and UBA2a-HA (hemagglutinin).