IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ canning-2002-1 UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Canning M., Kirby R., Finnegan D. Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. Mol. Genet. Genomics (1617-4615) 2002 canning-2002-2 UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Canning M., Kirby R., Finnegan D. Yeast strain used is EGY48 (not in CABRI). Embryonic cDNA library is used as prey. Mol. Genet. Genomics (1617-4615) 2002 ubcd4_drome Ubiquitin-conjugating enzyme E2-22 kDa Ubiquitin-protein ligase CG8284 Ubc4 E2 ubiquitin-conjugating enzyme 4 UbcD4 Ubiquitin carrier protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MANMAVSRIKREFKEVMRSEEIVQCSIKIELVNDSWTELRGEIAGPPDTPYEGGKFVLEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGGPHTFPDCDSKIQRLRDMGIDEHEARAVLSKENWNLEKATEGLFS q9w196_drome HECT-type E3 ubiquitin transferase Dmel\CG3356 CG3356 Dmel_CG3356 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFGFDGEYRRRPVQSLGGASHTCDRDTVIRKAALERQKRNELRQKENGAVVLQSYARSFIHRQRRKRAEREVFDIYLMGHKDGIVEDESLTFLLRRLNFFYSIREAKDSERLIEVCQQILRQPARLLQHSSPDSLWLLRLCKLLDTCLLQLSLSHTAQAIPLRMLETFTTVSSVQQYMKDEAVLFQYMERVFGFLIARNYFVRLRRLLDDKCPPLDGETLHAPSPLAEALLQLLLRPLEVAKRASAGGQMSSMSMAVCRNFTRDILATPHTDPLRYFVLPCFALNVDFPFDLLMRSLYDALESAGPAESDSTSSRRSFLFYGVETGHKTTRMDSIFSSFLLNSLMVLDRRQLATLHQSPLLVIYVRLIAEMMPNILQLPKSTLRGHANAPHRHRDGDDDSEESEDEDEELPAARTLDYDMEQTCRTSGELSVKERECLLESIAILNETERVDFIVQQLDPHIENTHLIYALCEICHNLMIYNKHAVFEYKLLYTLAFTPKFIRAVWFKLAAESTQLGFSAPLTLISKGVVPKHQGVDRTIPLLATFCMLFGRLLPTLHDVEFVENKLLLQVHSTINHVRLMPFSIAEIIQMSKTLKDISMGLVELAFPETRSNLANYRKVLGHTEADDKKLRHQKQIWANLLNVVVFVLNQIHTRDLRLGFCPEDHWTVTRLDLPLDRPTDLPLTHSSRLRGIRPFQPIRDFTREDFENGPPMSTKQIRSITILREIPFVVPFNKRVSILQSLVAASKMRVQGNMQAFLQGPSVLITVRRSHLYEDAYDKLRPDNEPDLRFKFRIQFVSSLGLDEAGIDGGGVFREFLSELIKTAFDPNRGFFMVTTDNKLYPNPNVADLFEDYEKHYYFIGRILGKSIYENLLVELPLAEFFLTKLAGKYSDVDIHQLASLDPELYRNLLYLKDYSGDVSELNLDFTVASSSLGQTQIVELKPQGQSIPVTNSNRIEYLQLIADYKLNVQIRRHCNAFRKGLSNVLPIEWLYMFSNKELQILISGAEIPIDLEDLKKHCEYGGEFSPEHPSIVTFWEVLEGFDDMQRRQLLKFVTSCSRPPLLGFKDLDPPFFIQNTGDMERLPTASTCTNLLKLPPFKTVEQMREKLLYAIQSGAGFELS psmd4_drome 26S proteasome non-ATPase regulatory subunit 4 Rpn10 26S proteasome regulatory subunit RPN10 26S proteasome regulatory subunit S5A 54 kDa subunit of mu particle Multiubiquitin chain-binding protein PROS-54 Pros54 p54 CG7619 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVLESTMICFDNSDFQRNGDYFPTRLIVQRDGINLVCLTKLRSNPENNVGLMTLSNTVEVLATLTSDAGRIFSKMHLVQPKGEINLLTGIRIAHLVLKHRQGKNHKMRIVVFVGSPINHEEGDLVKQAKRLKKEKVNVDIVSFGDHGNNNEILTAFINALNGKDGTGSHLVSVPRGSVLSDALLSSPIIQGEDGMGGAGLGGNVFEFGVDPNEDPELALALRVSMEEQRQRQESEQRRANPDGAPPTGGDAGGGGGVSGSGPGNEESAGAENEANTEEAMLQRALALSTETPEDNLPDFANMTEEEQIAFAMQMSMQDAPDDSVTQQAKRPKTDEANAPMDVDEDYSEVIGDPAFLQSVLENLPGVDPQSEAVRDAVGSLNKDKDKKSDGKDSQKK ubcd4-cg3356-1 yeast two hybrid assay using a LexA-UbcD4 fusion as bait 146258 N/A 146260 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 146261 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Authors have been unable to demonstrate co-immunoprecipitation of UbcD4 and CG3356 proteins in S2 cells so the significance of this Y2H interaction is not clear. ubcd4-pros54-1 yeast two hybrid assay using a LexA-UbcD4 fusion as bait 146258 N/A 146262 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 146260 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false ubcd4-pros54-2 UbcD4 co-immunoprecipitates with V5-tagged Pros54 146259 N/A 146260 unspecified role unspecified role prey prey act5c promoter uncategorized protein modification uncategorized protein modification n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. N/A 146262 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false act5c promoter uncategorized protein modification uncategorized protein modification n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association false false false