IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kutay-2000-4 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kutay U., Hartmann E., Treichel N., Calado A., Carmo-Fonseca M., Prehn S., Kraft R., Gorlich D., Bischoff FR. ulrike.kutay@bc.biol.ethz.ch J. Biol. Chem. (0021-9258) 2000 IMEx Immobilised hRan-GDP and HeLa cell extract used in the experiment. Only protein-protein interactions imex curation imex curation kutay-2000-1 Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom protein sequence protein sequence identification protein sequence Kutay U., Hartmann E., Treichel N., Calado A., Carmo-Fonseca M., Prehn S., Kraft R., Gorlich D., Bischoff FR. ulrike.kutay@bc.biol.ethz.ch J. Biol. Chem. (0021-9258) 2000 IMEx Immobilised hRan-GDP and HeLa cell extract used in the experiment. Only protein-protein interactions imex curation imex curation ima1_human Importin subunit alpha-1 KPNA2 RCH1 SRP1 Karyopherin subunit alpha-2 SRP1-alpha RAG cohort protein 1 protein protein human Homo sapiens Human MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF xpo2_human Exportin-2 Importin-alpha re-exporter Chromosome segregation 1-like protein Cellular apoptosis susceptibility protein CSE1L CAS XPO2 protein protein human Homo sapiens Human MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYIKRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQSGDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFSSLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQKYDEEFQRYLPRFVTAIWNLLVTTGQEVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVPNMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNPSVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANVNEFPVLKADGIKYIMIFRNQVPKEHLLVSIPLLINHLQAESIVVHTYAAHALERLFTMRGPNNATLFTAAEIAPFVEILLTNLFKALTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL imb1_human Importin subunit beta-1 Karyopherin subunit beta-1 Nuclear factor p97 Importin-90 KPNB1 NTF97 Pore targeting complex 97 kDa subunit protein protein human Homo sapiens Human MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL imb_xenla Importin subunit beta p97 Importin-90 Karyopherin subunit beta kpnb1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELVTILEKTVSPDRNELEAAQKFLEQAAVENLPTFVVELSKVLANPANSQVARVAAGLQIKNPLTSRDPDVKAQYQQRWLAIDASARGEIKTYVLRTLGTESYRPSSASQCVAGIACAEITVNQWPQLIPQLVANVTDPNSTERMKESTLEAIGYICQDIDPEQLQHKSNEILTAIIQGMRKEEPSNNVRLAATNALLNSLEFTKANFDKESERHYIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITVEAMKNEIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPESCQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPSSYCLSSSFEVIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQVESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGAEFLKYMEAFKPFLTIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEMMQFLLENLGNENVHRSVKPQILSVFGDVALAIGGEFKKYLDVVLNTLQQASQAQVDKSDYDMVDYLNELREGCIEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDSVVACGAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKTKTLATWATKELRKLKNQA o42480_xenla ipo7.L MGC52556 ipo7 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDPNILIEALRGTMDPALREAAERQLNESHKSLHFVSTLLQITMSEQLELPVRQAGVIYLKNMITQYWPDREVTPGELPPHTIPEEDRHCIRENIVEAIMHSPELIRVQLTTCIHHIIKHDYPNRWTAVVEKIGFYLQSDNSACWLGILLCLYQLVKNYEYKKPEERSPLIAAMQHFLPMLKDRYIQLLADPSEQSVLIQKQIFKIFYALVQYTLPLELINQQNLAEWIEILKTVVDRDVPAETLQVDEDDRPELPWWKCKKWALHILARLFERYGSPGNVSKEYNDFAEVFLKAFAVGVQQVLLKVLYQYKEKQYIAPRVLQQTLNYFNQGVSHAVTWKNLKPHIQGIIQDVIFPLMCYTDSDEDLWQEDPYEYIRMKFDVFEDFISPTTAAQTLLFTSCSKRKEVLQKTMGFCYQILTEPAADPRKKDGALHMIGSLAEILLKKKIYKDQMEFMLQNHVFPLFSSELGYMRARACWVLHYFCEVKFKVDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEKAKEYIVPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHLAMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKEITQQLEGICLQVIGTVLQQHVLEFYEEIFSLAHSLTCQQVSPQMWQLLPLVFDIFQQDGFDYFTDMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKKILTGVAGEDAECHAAKLLEVVILQCKGRGIDQVIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYSPPLLFNTLENLRFPNNEEPVTNHFIKQWLNDVDCFLGLHDRKICVLGLCALIELEQRPQVLNQMSSQILPAFLLLFNGLKRAYACHAEQENDSDDDGDGEDDEDAAELGSDEDDIDEEGQEYLEILAKQAGEDGDDEDWEDDDAEETALEGYTTLLDDEDTPIDEYQIFKAIFQKLQGRDPVWYQALTQGLNEDQGKQLQDIATLADQRRAAHESKMIEKHGGYKFNAPVVPSTFNFGNPAPGMN xpo2_xenla Exportin-2 cse1l xpo2 Chromosome segregation 1-like protein Importin-alpha re-exporter protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELSEGNLQGLTEYLKKTLDPDPAVRRPAEKYLESVEGNQNYPLLLLTLVERSQDNVIKVCSAVTFKNYIKRNWRIVEDESNKICEADRIAVKSSIINLMLRSPEQIQKQLSDAISIIGREDFPQKWPNLLTEMVNRFQSGDFHVINGVLHTAHSLFKRYRHEFKSSELWTEIKLVLDTFAGPLTDLFKATIELCNTHANDVGALKVLFSSLNLIAKLFHSLNFQDLPEFFEDNMETWMTNFHNLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQKYDEEFQPYLPRFVTAIWNLLVTTGQEVKYDLLVSNAIQFLASVCERPHYKNLFEDPSTLTSICEKVIVPNMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTNIFSGYVNSMLQEYAKNPSVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANINQYPVLKADGIKYIMFFRSQIPREQLLVTIPLLIAYLQAESIVVHTYAAHALERFFTMKGAATTTLIVAADMMPYVELLLANLFKALSLPGSTENEYIMKAIMRSFSLLQEAIIPYIPSVISQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITCRANPAAVASFEDALFLVFTEILQSDVQEFIPYVFQVMSLLLEIHTTDIPPSYMALFPHLLQPVLWERTGNIPPLVRLLQAYLERGATTIAASASDKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHLPAECIEQYKRQIFIVLFQRLQSSKTTKFVKSFLVFLNLFCIKFGAIALQEMFDSIQPKMFGMVVEKIIIPEIQKVSGPIEKKICAVGLTKVLTECPVMMDTEYTKLWTPLLQALIGLFELPEDDTIPDDEHFIDIEDTPGYQAAFSQLAFAGKKEHDPIGEMVNNPKILLAQSLHKLSTACPGRVPSMISTSLNAEALQFLQGYLQAGSVSLV kpna2-cse1l-1 Interaction determined by affinity chromatography. 97673 N/A 97675 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region zz tag zz tag ZZ tag ZZ tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 97676 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 97677 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 3 kpna2-cse1l-2 97673 N/A 97678 unspecified role unspecified role ancillary ancillary P62826:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 97675 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region zz tag zz tag ZZ tag ZZ tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 97676 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 3 ran-xpot-1 Interaction of the transport receptors with Ran determined by affinity chromatography. 97674 N/A 97679 unspecified role unspecified role prey prey xenla-ovum Xenopus laevis ovum egg cell N/A 97678 unspecified role unspecified role bait bait P62826:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 97680 unspecified role unspecified role prey prey xenla-ovum Xenopus laevis ovum egg cell N/A 97681 unspecified role unspecified role prey prey xenla-ovum Xenopus laevis ovum egg cell association association false false false Ran is GTP-bound. Importin-alpha was also reportedly observed but the given peptides could not be aligned to any know sequence. Fig. 2