MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint hoffman-2000-1 Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hoffman GR., Nassar N., Cerione RA. Cell (0092-8674) 2000 gdir1_bovin Rho GDP-dissociation inhibitor 1 Rho-GDI alpha ARHGDIA protein protein bovin Bos taurus (Bovine) Bovine MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDRTDHLSWEWNLTIKKEWKD p60953-2 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Placental protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL cdc42-gdir-1 X-Ray: Crystal structure of cdc42 in complex with GDI-1 99926 N/A 99927 unspecified role unspecified role neutral component neutral component gspgisggggilgl GSPGISGGGGGILGL tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false asp45 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ser47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr51 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp42 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false lys52 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false pro30 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ala31 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ser148 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp143 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false thr142 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr144 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gln130 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 99928 unspecified role unspecified role neutral component neutral component gly ser his tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false geranylgeranyl cys sgergercys S-geranylgeranyl-L-cysteine 2-amino-3-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenylthio)propanoic acid LIPID S-geranylgeranyl cysteine S-geranylgeranyl-L-cysteine (2R)-2-amino-3-([(2E,6E,10Z)-12-hydroxy-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]sulfanyl)propanoic acid Geranyl-geranyl GeranylGeranyl S-geranylgeranyl SGergerCys geranylgeranylc certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false thr35 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false thr35 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ala59 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr64 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr64 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg66 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his103 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his104 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false lys184 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg186 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 99950 99932 99951 99933 99952 99934 99953 99935 99954 99936 99955 99937 99956 99938 99957 99939 99958 99940 99959 99941 99960 99942 99961 99943 99964 99944 99965 99945 direct interaction direct interaction false false false working 25.7%, free 32.0% 2.60A The geranylgeranyl linked to cysteine 188 of Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like domain of GDI. N-terminal GST-tag of GDI is cleaved but some amino acids remains Arg186, Tyr175, Pro166, Thr132, Tyr110, Gln130, Trp194, Leu75, Phe102, Leu88, Ile112, Leu77, Leu196, Ile177, Ile198, Leu104, Ile169, Ile14, Leu11 of GDI form the hydrophobic pocket.