IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2000-1 Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. J. Biol. Chem. (0021-9258) 2000 kim-2000-2 Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. J. Biol. Chem. (0021-9258) 2000 kim-2000-3 Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. J. Biol. Chem. (0021-9258) 2000 ikkb_drome Inhibitor of nuclear factor kappa-B kinase subunit beta IKKbeta Cactus kinase IKK DIK IKK-like protein Immune response deficient protein 5 Lipopolysaccharide-activated kinase ird5 CG4201 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MITVVFCFSDCHGGIQILGRMSSVNKIKLNENNKMHSFGNWERCRNLGEGGFGLVIHWRNRTTGREIATKHIKEMGALSADQQVKLSERWNKELNWSRQFKNFPHIVAGVDIEDPDFLEYLNGMFSAKLPVIVLEYCNGGDVRKRLQSPENANGLTEFEVRQILGALRKALHFLHSQCGICHRDLKPDNIVIQRGVDGKKIYKLTDFGLARGTPDQTMVQSVVGTRHYYAPEVVENGFYNSTVDLWSFGVIAYELVTGELPFIPHQTLKNIILNLIKKPAKCIAITEDPEDNTRFVNQFELPQTHHLSRPWAAQFTKWLASPLNSNYKERGQLAANNVPVVFADLDKILNMNVLTIFAVNNCERLEYAVSAEMTMKDLIALIVLDTGMDEKELYFVLPTSHPHKTITPKSTPLQLYVEEWSDTSKDSRKWTKRSNPPVMLYIFQVKKECDYKIPEPILSILSRKFIANKFKTKERWLQKRVVLDMLYVLTKEQARYEMLVSGINERALSLEDEMMENSFIDSIDKQRIIISFAYDQLTSLLKEAQAKIPSRQLISSAQWEKLNRNYNFIIQSAKSIRSFLEACLREAKDMVKTTNQLRKEVCEKDLFDCARFYKKYLCNGAIISPSELNNDAEEFAKSRFKLYNEGEARHLPKSIDHMHYLYFKTKESIPVLLQQFCDIKKEIFQINLQMLMSASSTPPPKLELSAAMDRLAISSGSPSSDPFDSLRTINAIEEAERINNILVNEMKIDHY cact_drome NF-kappa-B inhibitor cactus CG5848 cact protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA ird5-ird5-1 Autophosphorylation detected by in-gel kinase assay. 131217 N/A 131220 enzyme enzyme unspecified role unspecified role drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. N/A 131220 enzyme target enzyme target substrate substrate unspecified role unspecified role drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. phosphorylation phosphorylation reaction phosphorylation false false false This reaction was LPS dependent. Ird5 was overexpressed in Drosophila Schneider cells. Auto-phosphorylation of Ird5. ird5-cact-1 Interaction detected by His-pull down. 131218 N/A 131220 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 131221 unspecified role unspecified role prey prey physical association physical association false false false Ird5 is six His tagged and overexpressed in Drosophila schneider cells. ird5-cact-2 Phosphorylation of Cact by Ird5 detected. 131219 N/A 131221 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 131220 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. phosphorylation phosphorylation reaction phosphorylation false false false Cact is phosphorylated.