IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gieseler-1999-1 In vitro interactions of Caenorhabditis elegans dystrophin with dystrobrevin and syntrophin. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gieseler K., Abdel-Dayem M., Segalat L. FEBS Lett. (0014-5793) 1999 dtn1_caeel Dystrobrevin-1 F47G6.1 dyb-1 protein protein caeel Caenorhabditis elegans MLWSNGGGGPREPSSAPSPDHHRAMHSVPPIVASEMQQLIDEMRLQDFDSIRFATYRAACKLRFIQQKTKVHLVDIWNMIEAFRENGLNALPLHTVIKTSRAELLLTTVFHNLNKRLVASQHVDTDVSISLLLAFLLGAYDKQNTGRLTVFSIKVALATLCAGKLVDKLRYIFSQIADSNGLMDHIKFTDFLQQILSLTTAVFEAPTFGFSENAVNQCFHKDEKVSLNVFLDTFLSDPCPPCIMWLPLLHRMASVSNVYHPVVCDACQVRSFTGFRYKCQRCANYQLCQSCFWRGRTSQNHSNEHEMKEYSSYKSPTKQLVHSIHKSLQCIPATSTVGDANIDIFNAKIGGPVSSKPARPLNLNNIVPATPTTIRRQHAATSSADWPTSPVLLPGQASHGGVIDDEHKLIARYAAKLSGRADYPLSNGRSMNSSMVGDERTLIAQLEEENSMMVREMARLESQTTSDDGLAGLRDRKMELEEKMFEMQQRRRELMMQLEHLMAQLNTGPQPSGGVSSASLSQLPFTASDQQLTGVNSTVANAFRAGSLPATSLQGDLLHAADQITTNMSDLVRQLDLAQSDENGVTINGF dmd_caeel Dystrophin-1 F15D3.1 dys-1 protein protein caeel Caenorhabditis elegans MLFSGASTAKPKKDEKKDKKSDRDPKNELQEWVFVRWANHLLGTERLTDYKSLQDGSNAIFVYQAIIGQTMAVLGNPSDDWPNVLQNIGDSKTNPQEVMEGQQKAVLSAWWQLVQFFWKNNAPVQLREEKLSEAIKQWCIEVMKSYEEIDVYDFTSSFRDGHAFNYLIHSYDRKLINLTKTAEMSAIDRIENAFAVAEKTWNVPRLLNPKDLHSDQLDSHSVLCYLMSLYLAMISTSKIETELEAQQIQQQKVAALLASHKMLSSQPSTSSSSALQIPPQTPPTAHHQAMLDRGKSFEQSAEGEVRSRKSSSSSQKSGKSKKARREEQLAEFKSCIEQVLTWLLEAEDELTTLTQMPRVELASVRSQFSDFESFMSSLTDSQDTVGRVLLRGQMLSNKSESEEEKESIGANLHLVNTRWEALREQAMQEQAVLQQQIHLLQQSELDTISQWLDAAELEIESFGPLAADSSQALRQIELHTKFQQKLNDFQETIDKLESFVAVVDEENDASVATLEDALSAVSVRWGHVCEWAEKRATKLDGLADLLDKTNEVFENLSGWLAERENELMTGLKSAHHLENEEQVAQQVRRLQKTEEQLEQEHASFVRLSQLSCELVGRLDDSNGAAANAVRLSLDSITQRWDNLVARIEEHGKTLVKSGKADVKQVQESQNEQKEQPASSEGLSTDTEGEEQKNQLVDKFLLHISKLSHELEPLQDWSEKFEVSRKKDDIRKMMNTCQEKLIQIKEQEARVNRLQLELEHLHVAKLNARQLKRANDAFEQFAKGWARIVTKISEAMNVLTGQEAGGNGNGSEEAAVAAKIEQWIEAVDKVINELSQLPVNERRSRIDKLEQQLQVQDKNVGFIEKDLLKKAILKKGLEIAGKRLAALKVEEKPVEKEEQLVLSNSEEPEAEKHVTFVQETTEKPAPLQEPTSEAQLLEELDGPWSRVGDVVAIEHDLLRAKRAVDTARNSQMSNETVEKAETRKAEMEEKRRVTMSARSKFRMAEETLEEIERNLDRLQVSDLEIADLVRGLEQEAAKLGERVSQRKEAERTAEKILSMDDDEISQEIVIKTKDSTEKLIKRWNQLELDLEENLRKAKRDQDVFIQKRLREGEEALNEIKTAIEGKRESLDAETAAENLDHLESSLDNISSLFGEIGSLPMDDNSREKLSKLAKAKDQITARANEALAALTRTVSECEDFEKQIMLFQNWSARIGFLLQARKSADISAFDIPHEYHEDLGNEAELIPKLSREFEEWTVKLNEMNSTATEKDDSARMREQLNHANETMAELKRKFNEFKRPKGFEEKLEKVITTLSNVEMGLDDTTGIDGSECGGALMEVRALVRMLDGAQEKWKDLAENREQLVKDRVLDEETSKETLQKLQYAKTKSKELYERSSTCIERLEDCVEMYQRLKMESDEIERFLEEMEGKLDQYAASDRPEEAEIVNELISEWNRNEAAMKNAEHLQRQLNERAIKIPDDVLSLKRLRADALKNRLNSWCRTIQEMSEDDESALLEIDELHQNLEKELKLVSDKEPSKIAEKLRFLRADRDRLSSRTRKLAAKNPRLAATSSDVLAGLNQKWKELEVKASAEKAPAPELRDARLSSPSEQPFDKRVQELCDLFENLEAQLDFNGSPVSMVTEYQKRVENLDEYLDEYRPALDDTIEEGRKIAETGRLELQTHSAIEKLDELTNRIEQVEVELDKHRDKVPSLVEQHEQLKKDIDSFLLVLDVFTDRNLDDVDIAKSTRKELAERDSHIVSLTSRATAIHCALPGKGPQLHDVTLDKLRDRIEKLEARLSATEKKPVETVKSTIPDRPEVPEEPEKSSPDRTSRSSLQLAMEAYGTATEDDSVISEAVTVGQKSVDQVDPVEQLEPVEPVEPKLEVKQLKDEATEEEEKRTIILPDETEKVIETIPAARPSAGPSEGTVAEVSTSEILKARPAQESIERTVREVPVDEYEETANISSGDELQDHKISSAVPDSESEIASMFEVLDSIEDSHTNFEEFPFDYLDSADDDLKKTLLKLESCEKTLAKNEMTINIAQAENARERITMLRQMALQRKDKLPKFNEEWNAMQELIQLADALVDEAERYESDQIPQMDRKSAPNVLGELRKRVANAEGPVIDLVKKLSQLVPRMQEDSPKSQDIRQKVYGIEDRFRRVGQAEGAAISKALSSALTEPELKLELDEVVRWCEMAEKEAAQNVNSLDGDGLEKLDGRLAQFTKELQERKDDMVQLEMAKNMIIPSLKGDAHHDLRRNFSDTAKRVAMVRDELSDAHKWVATSRDTCDTFWADIDSLEQLARDVVRRANGIRMAVIYTPSRENVEGVLRDVQRLKMSIGDVKKRVQTANLPPAIKLAGKNAKRVVQVLTETATTIADCHDIPTYLIDEMNDSGGDTTESRSTVVEMTSVHTKQSSSSSSNKTPSAGGESDDAHTLNGDDEQSEEDQKIYSRESSSTLPRGVSSLGSTGSSGVLDPVAVQLTHTRHWLHDVERDASITVDLAQWQPARELWQSIQGIIDEIRLRSVHVTGAHDASPNRQVRQQAAQLLTEMRRTIENCEKRCLILNQISDIARQNEASRNEMELWLKSASDVIGERRVEELSEEVVRQELQVLERVVEQLTERKDKMAEINSQANKIVDTYTKDEAHNLSHLLSRLNMSWTKFNDNIRIRRAVLEASLRSRRDFHSALSEFEKWLSRQEDNCSKLSADTSNHQAIKDTSKRKNWTQSFKTLNAELNAHEDVMKSVEKMGKMLAESLESGNEKVELLKRVGETTRRWTALRKTTNEIGERLEKAEQEWEKLSDGLADLLSWVEAKKQAIMDEQPTGGSLSAVMQQASFVKGLQREIESKTANYKSTVEEAHSFLMQHDLRPKLHSPHVLDDDYEKEELANLEQRRRGLEINANCERLKKNWAELGIEVESWDKLVQHAMQRLQELERNLAECQLHLTSSENEIETMKAVEKIHLEDLKIAREETDQISKRIDEVRLFVDDVNDAAARLLAEDLKLDEHAKGQIEHVNKRYSTLKRAIRIRQAAVRNAASDFGPTSEHFLNQSVTLPWQRAISKSNLLPYYIEQTSEKTQWEHPVWVEIVKELSQFNRVKFLAYRTAMKLRALQKRLCLDLVDLTLLEKAFVRLKGLSAEECPGLEGMVCALLPMYEALHAKYPNQVQSVSLAVDICINFLLNLFDQSRDGIMRVLSFKIAMIVFSNIPLEEKYRYLFKLVSQDGHATQKQIALLLYDLIHIPRLVGESAAFGGTNVEPSVRSCFETVRLAPTISEGAFIDWVKKEPQSIVWLAVMHRLVISESTKHASKCNVCKMFPIIGIRYRCLTCFNCDLCQNCFFSQRTAKSHRTNHPMQEYCEKTTSSDDARDFAKMIRNKFRASKRQKGYLPIDVAEEGIPLTCPPAKVTNQATEQMNADTSQMTAHLAKLSAEHGGGAEHMEPVQSPLQIINQVEQLQRDEMDQMLHRLQFENKQLRKELEWKRGAASTMEIDRSSKRHQERHQSESRGGTLPLRNGRSVVSLKSTQSQNDVMDEAKALRLHKQRLEHRSRILEQQNEQLEMQLQRLKKVIDAQKQQAPLSTNSLLRGSHHQPWSPERARSGSASTLDRGLIVSSRHQEQAEAAGGGAEDSSDEAGGAGGGPRGSSVGQMQNLMTACDDLGKAMESLVVSVVYDSDDEEND snt1_caeel Syntrophin-1 F30A10.8 stn-1 protein protein caeel Caenorhabditis elegans MAAVRSGLVDIFVQGQWHRVLATLDPTAITLQTMEQNEAEAEKRTVRVVKYDGNGLGISIKGGRDNNMPIVISKIFKGMAADQAGELFLDDVIISVNGENLLDASHEEAVRALKRAGRVVDLQVQYRREDMMHRENIVENVEWDDDIRERVRTIGLKLAYVARAGIDADAEGRILEMRSPSGRYSLAMRCSSSEEADGWFEALHACTTCLLTQALAQVNIMLGNNPQVRHMGWIAEQVSENGISMWKPKFMTLTNSEILFYEAVPQLKAEWAEPRLVRPLVATRVVQTSSRTAPVIKGLTDVISFRMRTGTQQGVRTHTIRVETHAELARWVRAVVIGGYEACLSTSQVSAPCLWRGESCELIVNLDNGISLLSSTGEVLWQHSFETIRATGDDGGRFLWVDFGPPHGEQELDLLNSAKPVVFILHSFLATKVYRLGLYA dyb1-dys1-1 Interaction between dyb1 and deletions of dys1 145750 N/A 145751 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 145752 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false The region between AA 3531-3618 within the C-terminal of dys-1 is necessary for interaction with dyb-1. dys1-dyb1-2 Interaction between dys1 and deletions of dyb1 145750 N/A 145752 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 145751 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false The region between AA 478 to 521 of dyb-1 is necessary for interaction with dys-1. This interaction is confirmed by Y2H assay in PMID: 11895430 stn1-dyb1-3 Interaction between stn1 and deletions of dyb1 145750 N/A 145751 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 145753 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false The region between AA 391-450 of dyb-1 is necessary for interaction with stn-1. This interaction is confirmed in the Y2H system as in PMID 14499607. stn1-dys1-4 Interaction between stn1 and deletions of dys1 145750 N/A 145752 unspecified role unspecified role bait bait c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 145753 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false The regions between AA 3402-3473 and 3531-3674 of dys-1 are necessary for interaction with stn-1. This interaction was not found using the Y2H method as described in PMID 14499607