MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint madania-1999-2 The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B. The bait is fused to IgG binding domain of protein A and expressed in yeast. It can then be immunoprecipitated using IgG-sepharose. fig 9. Mol. Biol. Cell (1059-1524) 1999 madania-1999-1 The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B. The bait is fused to IgG binding domain of protein A and expressed in yeast. It can then be immunoprecipitated using IgG-sepharose. fig 9. Mol. Biol. Cell (1059-1524) 1999 act_yeast Actin ACT1 ABY1 END7 YFL039C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF 87AC19B0B0BC9E71 las17_yeast Proline-rich protein LAS17 LAS17 YOR181W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLLNSSDKEIIKRALPKASNKIIDVTVARLYIAYPDKNEWQYTGLSGALALVDDLVGNTFFLKLVDINGHRGVIWDQELYVNFEYYQDRTFFHTFEMEECFAGLLFVDINEASHFLKRVQKRERYANRKTLLNKNAVALTKKVREEQKSQVVHGPRGESLIDNQRKRYNYEDVDTIPTTKHKAPPPPPPTAETFDSDQTSSFSDINSTTASAPTTPAPALPPASPEVRKEETHPKHSLPPLPNQFAPLPDPPQHNSPPQNNAPSQPQSNPFPFPIPEIPSTQSATNPFPFPVPQQQFNQAPSMGIPQQNRPLPQLPNRNNRPVPPPPPMRTTTEGSGVRLPAPPPPPRRGPAPPPPPHRHVTSNTLNSAGGNSLLPQATGRRGPAPPPPPRASRPTPNVTMQQNPQQYNNSNRPFGYQTNSNMSSPPPPPVTTFNTLTPQMTAATGQPAVPLPQNTQAPSQATNVPVAPPPPPASLGQSQIPQSAPSAPIPPTLPSTTSAAPPPPPAFLTQQPQSGGAPAPPPPPQMPATSTSGGGSFAETTGDAGRDALLASIRGAGGIGALRKVDKSQLDKPSVLLQEARGESASPPAAAGNGGTPGGPPASLADALAAALNKRKTKVGAHDDMDNGDDW 4488355563AA2645 rv167_yeast Reduced viability upon starvation protein 167 D9509.8 YDR388W RVS167 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFKGFTKAVSRAPQSFRQKFKMGEQTEDPVYEDAERRFQELEQETKKLSEESKRYSTAVNGMLTHQIGFAKSMEEIFKPISGKMSDPNATIPEDNPQGIEASEQYRAIVAELQETLKPDLALVEEKIVTPCQELLKIITYIRKMATKRNHKKLDLDRHLNTYNKHEKKKEPTAKDEERLYKAQAQVEVAQQEYDYYNDLLKTQLPILFSLEAEFVKPLFVSFYFMQLNIFYTLYNRLQDMKIPYFDLNSDIVESYIAKKGNVEEQTDALTITHFKLGYSKAKLEMTRRKYGVATAEGSPVSGASSGVGYGAGYDPATATSPTPTGYGYGAAAPSYAAQPAAQYGTAAAVGTAAAVGTAAGAAAGAVPGTYPQYAAAQSPPLTGLGFQQSPQQQQGPPPAYSNPLTSPVAGTPAAAVAAAPGVETVTALYDYQAQAAGDLSFPAGAVIEIVQRTPDVNEWWTGRYNGQQGVFPGNYVQLNKN 3F0AB53EBCC95A5B vrp1_yeast Verprolin VRP1 END5 MDP2 YLR337C L8300.13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGAPAPPPPPPPPALGGSAPKPAKSVMQGRDALLGDIRKGMKLKKAETNDRSAPIVGGGVVSSASGSSGTVSSKGPSMSAPPIPGMGAPQLGDILAGGIPKLKHINNNASTKPSPSASAPPIPGAVPSVAAPPIPNAPLSPAPAVPSIPSSSAPPIPDIPSSAAPPIPIVPSSPAPPLPLSGASAPKVPQNRPHMPSVRPAHRSHQRKSSNISLPSVSAPPLPSASLPTHVSNPPQAPPPPPTPTIGLDSKNIKPTDNAVSPPSSEVPAGGLPFLAEINARRSERGAVEGVSSTKIQTENHKSPSQPPLPSSAPPIPTSHAPPLPPTAPPPPSLPNVTSAPKKATSAPAPPPPPLPAAMSSASTNSVKATPVPPTLAPPLPNTTSVPPNKASSMPAPPPPPPPPPGAFSTSSALSASSIPLAPLPPPPPPSVATSVPSAPPPPPTLTTNKPSASSKQSKISSSSSSSAVTPGGPLPFLAEIQKKRDDRFVVGGDTGYTTQDKQEDVIGSSKDDNVRPSPISPSINPPKQSSQNGMSFLDEIESKLHKQTSSNAFNAPPPHTDAMAPPLPPSAPPPPITSLPTPTASGDDHTNDKSETVLGMKKAKAPALPGHVPPPPVPPVLSDDSKNNVPAASLLHDVLPSSNLEKPPSPPVAAAPPLPTFSAPSLPQQSVSTSIPSPPPVAPTLSVRTETESISKNPTKSPPPPPSPSTMDTGTSNSPSKNLKQRLFSTGGSTLQHKHNTHTNQPDVDVGRYTIGGSNSIVGAKSGNERIVIDDSRFKWTNVSQMPKPRPFQNKTKLYPSGKGSSVPLDLTLFT 24C7522D5B1CA1C8 lsb5_yeast LAS seventeen-binding protein 5 YCL186 YCL34W YCL034W LSB5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGFLSDHPHTAITETIFRIVSSRDYTLEVELAPLIQLIKADHNDYNYTVNQEEAARALRKKIKYGNRLQQSRTLDLLDLFISQGVKFTVMYNDDKLLQRLRGMATNSENSGSGEKYEPRIIKKCAAYAISWLNYITQNNLENARAYSGLYQLGQTVKQRYSKSSRSRRSGGGSGGRSNFMDDSADDTLYQSNSLTSADRLYRIPQINMNKEAPRIRLIISDALASAVSLQNSLIGLPKGKFSTDDEEATSKFIQARAIRRKVLRYLQLVTEGEFLGSLIHANDELVAALTAYDDRSAQDDSSDESDHGSYDDGIYDENEQDNSRYIDSESSEEESLSSYQPSTISNPFGDHNKI lsb6_yeast Phosphatidylinositol 4-kinase LSB6 LSB6 YJL100W LAS seventeen-binding protein 6 J0834 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNEAYQHDHTVNPHQKIVVNSYDWLQFRDEQDHCKSKNPITHASPGVGSNAQNSDIAEAPQVFHPSYQSLVNVPSESPRPDQTSGSNPAVGLLHNAEDKASGQEEEGSQYEIQYSVFRPLHAYPTKGLAYEQLRRKEEQEQRENFNHLVSDCIEAVETFGRELERIQTGSSGSYFVYGTRADESVPVGVFKPKDEEPYGPFSPKWTKWAHRTFFPCLFGRSCLIPNLGYICESAASLLDRRLETHLVPYTDTASIESFNFYDNRKKWVLGYNLQKKKQKKLGSFQLFLKEYINADEFFHKYPLPGMYSDVKHSFHRKSSGEDINHKPETTRNLTDETEPSKQINSSPISTESEENSKFEWTESSLSQFRLELEKLIILDYIMRNTDRGLDNWMVKLIKLSNNKWRLKLAAIDNGLSFPWKHPDEWRLYPYGWLYLPLQLLAKPFSEQMRSHFLPILTSTNWWEESYQEFLALFSRDQDFNVRMWKKQWAVLKGQAFNVVETLKDPRQGPLELVRRTRCQVIDEKMQVPCCPPPVSIFKNAIDEPIGSYSTSPMVLPSTPSTIPFHAHNQSNSNPVYYDSTLHPFANKTVIAERLQIVNSTPVFTWC pin3_yeast [PSI+] inducibility protein 3 PIN3 LAS seventeen-binding protein 2 LSB2 YPR154W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSASLINRSLTNIRTELDFLKGSNVISNDVYDQINKSLPAKWDPANAPRNASPASLEYVEALYQFDPQQDGDLGLKPGDKVQLLEKLSPEWYKGSCNGRTGIFPANYVKPAFSGSNGPSNLPPPPQYKAQELQQIPTQNSAASSYQQQPFPPPSTNYYQQPQQQPQQAPPPQQQQQQQQHQSSHSHLKSFGSKLGNAAIFGAGASIGSDIVNNIF lsb3_yeast LAS seventeen-binding protein 3 LSB3 YFR024C YFR024C-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MGINNPIPRSLKSETKKAAKILASFVKPNQVFGADQVIPPDVLKRAKGLAIITILKAGFLFSGRAGSGVIVARLKDGTWSAPSAIAMAGAGAGGMVGIELTDFVFILNTQDAVKSFSEFGTITLGGNVSVSAGPLGRSAEAAASASAGGVAAVFAYSKSKGLFAGVSVEGSAIIERREANRKFYGDNCTAKMILSGRIRPPPAVDPLFRVLESRAFNYRPSNGGRGSFDDDEDDYYDDDDYYNDIPSSFSSTDASSTRPNTRSTRRRAQSGSRYTFDDDDDDDDYGTGYSRNSRLAPTNSGGSGGKLDDPSGASSYYASHRRSGTAQSRARSSRNRWADDEYDDYDDDYESGYRRGNGRDRTKDREVDDLSNRFSKSRISSASTPQTSQGRFTAPTSPSTSSPKAVALYSFAGEESGDLPFRKGDVITILKKSDSQNDWWTGRVNGREGIFPANYVELV F486C787B5771AAC lsb1_yeast LAS seventeen-binding protein 1 LSB1 YGR136W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSASLVNRSLKNIRNELEFLKESNVISGDIFELINSKLPEKWDGNQRSPQNADTEEYVEALYDFEAQQDGDLSLKTGDKIQVLEKISPDWYRGKSNNKIGIFPANYVKPAFTRSASPKSAEAASSSTVSRPSVPPPSYEPAASQYPSQQVSAPYAPPAGYMQAPPPQQQQAPLPYPPPFTNYYQQPQQQYAPPSQQAPVEAQPQQSSGASSAFKSFGSKLGNAAIFGAGSAIGSDIVNSIF 5F0B1361AF84AA79 ysc84_yeast Protein YSC84 YSC84 LSB4 LAS seventeen-binding protein 4 YHR016C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGINNPIPRSLKSETKKAAKVLRSFVKPNQVFGADQVIPPYVLKRAKGLAIITVLKAGFLFSGRAGSGVIVARLKDGTWSAPSAIAMAGAGAGGMVGVELTDFVFILNSEEAVRSFSEFGTITLGGNVSVSAGPLGRSAEAAASASTGGVSAVFAYSKSKGLFAGVSVEGSAILERREANRKFYGDNCTSKMILSGRVKVPPAADPLLRILESRAFNFTRHDHDDNASGDDFYDDGQYSDNTSHYYDDIPDSFDSTDESSTRPNTRSSRRRGMSLGSRSRYDDDYDDDGYGRGRGYGDFDSEDEDYDYGRSPNRNSSRNRGPQIDRGTKPRANTRWEDDLYDRDTEYSRPNHSGRDYDNTRGNRRGYGRERGYSLGHGPTHPSNMSNVDDLSHKMSKTGLGNESTATNSATPTAVALYNFAGEQPGDLAFKKGDVITILKKSDSQNDWWTGRTNGKEGIFPANYVRVS DE42E8ECF5B83C92 arp2_yeast Actin-related protein 2 ACT2 YDL029W D2778 ARP2 Actin-like protein ARP2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDPHNPIVLDQGTGFVKIGRAGENFPDYTFPSIVGRPILRAEERASVATPLKDIMIGDEASEVRSYLQISYPMENGIIKNWTDMELLWDYAFFEQMKLPSTSNGKILLTEPPMNPLKNREKMCEVMFEKYDFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLSHLTRRLDVAGRDVTRHLIDLLSRRGYAFNRTADFETVRQIKEKLCYVSYDLDLDTKLARETTALVESYELPDGRTIKVGQERFEAPECLFQPGLVDVEQPGVGELLFNTVQSADVDIRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWFSRVLHNDPSRLDKFKVRIEDPPRRKHMVFIGGAVLASIMADKDHMWLSKQEWQESGPSAMTKFGPR 4C9E8952B477A1B9 act1-act1-1 Y2H: bait= actin, prey = actin 141233 N/A 141235 unspecified role unspecified role bait bait N/A 141235 unspecified role unspecified role prey prey physical association physical association false false false las17-rvs167-4 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = Rvs167p 141233 N/A 141236 unspecified role unspecified role bait bait N/A 141237 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false las17-vrp1-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = vrp1p 141233 N/A 141238 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 141236 unspecified role unspecified role bait bait physical association physical association false false false las17-lsb5-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb5 141233 N/A 141239 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 141236 unspecified role unspecified role bait bait physical association physical association false false false las17-lsb6-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb6 141233 N/A 141240 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 141236 unspecified role unspecified role bait bait physical association physical association false false false las17-pin3-2 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb2 (pin3, YPR154W) 141233 N/A 141236 unspecified role unspecified role bait bait N/A 141241 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false las17-yfj4-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb3 (YFR024C) 141233 N/A 141242 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 141236 unspecified role unspecified role bait bait physical association physical association false false false las17-yg3d-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb1 (YGR136W) 141233 N/A 141236 unspecified role unspecified role bait bait N/A 141243 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false las17-yhh6-1 Y2H: bait= bee1p (wasp yeast homolog, las17p), prey = lsb4 (YHR016C) 141233 N/A 141236 unspecified role unspecified role bait bait N/A 141244 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false las17-act1-2 Y2H: bait= actin, prey = bee1p (wasp yeast homolog, las17p) 141233 N/A 141236 unspecified role unspecified role prey prey N/A 141235 unspecified role unspecified role bait bait physical association physical association false false false rvs167-act1-3 Y2H: bait= actin, prey = Rvs167p 141233 N/A 141237 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 141235 unspecified role unspecified role bait bait physical association physical association false false false las17-arp2-1 CoIP: bait= bee1p (wasp yeast homolog, las17p), prey = arp2p 141234 N/A 141245 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 141236 unspecified role unspecified role bait bait igg binding domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false