IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ probst-1997-1 Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Probst MR., Fan CM., Tessier-Lavigne M., Hankinson O. J. Biol. Chem. (0021-9258) 1997 probst-1997-2 Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein. in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Probst MR., Fan CM., Tessier-Lavigne M., Hankinson O. J. Biol. Chem. (0021-9258) 1997 arnt_mouse Aryl hydrocarbon receptor nuclear translocator Arnt Dioxin receptor, nuclear translocator Hypoxia-inducible factor 1-beta protein protein mouse Mus musculus Mouse MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRRSGLDFDDEVEVNTKFLRCDDDQMCNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRVLDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDPVSMNRLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKTREWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIPRSQLGPTANLSLEMGTGQLPSRQQQQQHTELDMVPGRDGLASYNHSQVSVQPVASAGSEHSKPLEKSEGLFAQDRDPRFPEIYPSITADQSKGISSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQILAQISRHSNPAQGSAPTWTSSSRPGFAAQQVPTQATAKTRSSQFGVNNFQTSSSFSAMSLPGAPTASSGTAAYPALPNRGSNFPPETGQTTGQFQARTAEGVGVWPQWQGQQPHHRSSSSEQHVQQTQAQAPSQPEVFQEMLSMLGDQSNTYNNEEFPDLTMFPPFSE ahr_mouse Aryl hydrocarbon receptor Ahr protein protein mouse Mus musculus Mouse MSSGANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSTPADRNGGQDQCRAQIRDWQDLQEGEFLLQALNGFVLVVTADALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPDSAQGVDEAHGPPQAAVYYTPDQLPPENASFMERCFRCRLRCLLDNSSGFLAMNFQGRLKYLHGQNKKGKDGALLPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGQLILGYTEVELCTRGSGYQFIHAADMLHCAESHIRMIKTGESGMTVFRLFAKHSRWRWVQSNARLIYRNGRPDYIIATQRPLTDEEGREHLQKRSTSLPFMFATGEAVLYEISSPFSPIMDPLPIRTKSNTSRKDWAPQSTPSKDSFHPSSLMSALIQQDESIYLCPPSSPAPLDSHFLMGSVSKCGSWQDSFAAAGSEAALKHEQIGHAQDVNLALSGGPSELFPDNKNNDLYNIMRNLGIDFEDIRSMQNEEFFRTDSTAAGEVDFKDIDITDEILTYVQDSLNNSTLMNSACQQQPVTQHLSCMLQERLQLEQQQQLQQPPPQALEPQQQLCQMVCPQQDLGPKHTQINGTFASWNPTPPVSFNCPQQELKHYQLFSSLQGTAQEFPYKPEVDSVPYTQNFAPCNQPLLPEHSKSVQLDFPGRDFEPSLHPTTSNLDFVSCLQVPENQSHGINSQSTMVSPQAYYAGAMSMYQCQPGPQRTPVDQTQYSSEIPGSQAFLSKVQSRGIFNETYSSDLSSIGHAAQTTGHLHHLAEARPLPDITPGGFL sim1_mouse Single-minded homolog 1 Sim1 protein protein mouse Mus musculus Mouse MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKQGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISASKPTFSYTSSSTPTISDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRSSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPTGRDPWWGSRAALPLTKASPESREAYENSMPHITSIHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPTGEVCHSSALASTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS sim2_mouse Single-minded homolog 2 Sim2 SIM transcription factor protein protein mouse Mus musculus Mouse MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRTGPLDSVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVLTAHPPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLHSNMFMFRASLDLKLIFLDSRVTELTGYEPQDLIEKTLYHHVHGCDTFHLRYAHHLLLVKGQVTTKYYRLLSKLGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTDVEYKELQLSLDQVSTSKSQESWRTTLSTSQETRKSAKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECSQVGNWRTSPPTNAVAPPEQQLHSEASDLLYGPPYSLPFSYHYGHFPLDSHVFSSKKPGLPAKFGQPQGSPCEVARFFLSTLPASSECQWHCANSLVPSSSSPAKNLSEPSPVNAARHGLVPNYEAPSAAARRFCEDPAPPSFPSCGHYREEPALGPAKAPRQASRDAARLALARAPPECCAPPAPEPQAPAQLPFVLLNYHRVLARRGPLGSAAPGAPEAAGSLRPRHPGPVAASAPGAPRPHYLGASVIITNGR hs90a_mouse Heat shock protein HSP 90-alpha Hsp90aa1 Hsp86 Hsp86-1 Hspca Heat shock 86 kDa Tumor-specific transplantation 86 kDa antigen protein protein mouse Mus musculus Mouse MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPSKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKHSQFIGYPITLFVEKERDKEVSDDEAEEKEEKEEEKEKEEKESDDKPEIEDVGSDEEEEEKKDGDKKKKKKIKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWEEHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQKHIYFITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTVDDTSAAVTEEMPPLEGDDDTSRMEEVD arnt-ahr-1 Interaction of their HLH>PAS domains studied by Y2H. 16120 N/A 16122 unspecified role unspecified role bait bait N/A 16123 unspecified role unspecified role prey prey physical association physical association false false false Interaction ligand-dependent. ARNT aa 70-474 used. AHR 1-397 used. Simmilar results obtained when AHR used as bait. arnt-sim1-1 Interaction between the HLH.PAS domains studied by Y2H. 16120 N/A 16122 unspecified role unspecified role bait bait N/A 16124 unspecified role unspecified role prey prey physical association physical association false false false ARNT AA70-474 and SIM1 AA 1-363 used in the experiment. Simmilar interaction observed when SIM1 is bait arnt-sim2-1 Interaction in the HLH.PAS domains studied by Y2H 16120 N/A 16122 unspecified role unspecified role bait bait N/A 16125 unspecified role unspecified role prey prey physical association physical association false false false ARNT AA 70-474 and SIM2 AA 1-365 used in the study. marginal interaction observed. Simmilar results if SIM2 was used as bait. arnt-sim1-2 Interaction detected by coimmunoprecipitation. 16121 N/A 16122 unspecified role unspecified role bait bait in vitro In vitro N/A 16124 unspecified role unspecified role prey prey region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false hspca-sim1-1 Interaction detected by coimmunoprecipitation. 16121 N/A 16126 unspecified role unspecified role bait bait in vitro In vitro N/A 16124 unspecified role unspecified role prey prey region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false hspca-ahr-1 Interaction detected by coimmunoprecipitation. 16121 N/A 16126 unspecified role unspecified role bait bait in vitro In vitro N/A 16123 unspecified role unspecified role prey prey region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false