MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint kaiser-2003-3 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Size-selected (350–700 bp) cDNA fragments from 9.5 to 10.5-day-old whole mouse embryos J. Biol. Chem. (0021-9258) 2003 IMEx Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Only protein-protein interactions imex curation imex curation kaiser-2003-4 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. J. Biol. Chem. (0021-9258) 2003 IMEx Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Only protein-protein interactions imex curation imex curation kaiser-2003-1 The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. J. Biol. Chem. (0021-9258) 2003 IMEx Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Only protein-protein interactions imex curation imex curation kaiser-2003-2 The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Size-selected (350–700 bp) cDNA fragments from 9.5 to 10.5-day-old whole mouse embryos J. Biol. Chem. (0021-9258) 2003 IMEx Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ., Kaiser FJ., Möröy T., Chang GT., Horsthemke B., Lüdecke HJ. Only protein-protein interactions imex curation imex curation trps1_mouse Zinc finger transcription factor Trps1 Trps1 protein protein mouse Mus musculus Mouse MVRKKHPPLRNVASEGEGQTLEPTATESKVSGKNKELSADQMSENTDQSDVAELNSKEEHSTHGQEPSSSGKKDLQISGLSEKAGFNYESPSKGGSLVSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEEHKMSPKATEETGPVQSGQANCQGLSPVSVASKNPQVPSDGGVRLSKPKGDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQKVNRSVLSGVLQDISSSRPALLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTHPNKIKVSLPSSEGVKPSEKNSNKSIPALRASDSGDVGKWQDKMTVKAGDDTPVGYSVPIKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLPRGSVINQNDLAKSVEGEPLTKPEKGLSGAKKKDFPSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLHNIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGVAGSSRVKHQCHQCSFSTPDVDVLLFHYETVHESQASDVKQEANHLLGSDGQQAVRDSKEHSCTKCDFITQVEEEISRHYRRAHSCYKCRQCSFTAADTQSLLEHFNTVHCQEQEITTANGEEGGHAIPTIKEEPKIDLKVYSLLNPDSKMGETVPESIVKREKLDDKEGLKDKIWTESSTDDLRGVAWRGADILRGSPSYTQASLGLLTPVSSSQEQTKTLRDSPNVEAAHLARPMYGLAVDTKGFLQGAPAGSEKSASLTQQYPASGESKTKDESQSLLRRRRGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSGEEQVNGSPLERRSEDHLSESHPREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQALDIHKRMQPLHIQIKSPQESTGDPGNSSSVSDGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGVPFVHNDFQSEADWLRFWSKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGASKEKTKAPPTVKNEDPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKYDFTTHIQRGLHRNNAQAEKNGKPKE rnf4_mouse E3 ubiquitin-protein ligase RNF4 Rnf4 RING finger protein 4 RING-type E3 ubiquitin transferase RNF4 protein protein mouse Mus musculus Mouse MSTRNPQRKRRGGTVNSRQTQKRTRETTSTPEVSLETEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSRDKDVYVTTHTPRSTKDDGATGPRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI trps1_human Zinc finger transcription factor Trps1 Tricho-rhino-phalangeal syndrome type I protein Zinc finger protein GC79 TRPS1 protein protein human Homo sapiens Human MVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIPALQSSDSGDLGKWQDKITVKAGDDTPVGYSVPIKPLDSSRQNGTEATSYYWCKFCSFSCESSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSRGSVINQNDLAKSSEGETMTKTDKSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLHNIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSRVKHQCHQCSFTTPDVDVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKEHSCTKCDFITQVEEEISRHYRRAHSCYKCRQCSFTAADTQSLLEHFNTVHCQEQDITTANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMGEPVSESVVKREKLEEKDGLKEKVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTLRDSPNVEAAHLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRRRGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPEALQAEQLNKQQRGSNEEQVNGSPLERRSEDHLTESHQREIPLPSLSKYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQESTGDPGNSSSVSEGKGSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSEADWLRFWSKYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSRPGPTANGASKEKTKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKYDFTTHIQRGLHRNNAQVEKNGKPKE rnf4_human E3 ubiquitin-protein ligase RNF4 SNURF RING finger protein 4 Small nuclear ring finger protein RNF4 RES4-26 RING-type E3 ubiquitin transferase RNF4 protein protein human Homo sapiens Human MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI trps1-rnf4-3 13646 N/A 13650 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 N/A 13651 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 13660 13657 physical association physical association false false false Fig. 3 homomint mint trps1-rnf4-4 13647 trps1_human 13652 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 rnf4_human 13653 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association false false false Fig. 4E homomint mint trps1-rnf4-1 13648 trps1_human 13652 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 rnf4_human 13653 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false Fig. 4C homomint mint trps1-rnf4-2 13649 trps1_human 13650 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 rnf4_human 13651 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 13675 13672 physical association physical association false false false Fig. 2 homomint mint