IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ taru-2002-2 Interaction of Alzheimer's beta -amyloid precursor family proteins with scaffold proteins of the JNK signaling cascade. in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Taru H., Iijima K., Hase M., Kirino Y., Yagi Y., Suzuki T. Alzheimers - Interactions investigated in the context of Alzheimers disease J. Biol. Chem. (0021-9258) 2002 Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. taru-2002-1 Interaction of Alzheimer's beta -amyloid precursor family proteins with scaffold proteins of the JNK signaling cascade. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Taru H., Iijima K., Hase M., Kirino Y., Yagi Y., Suzuki T. Alzheimers - Interactions investigated in the context of Alzheimers disease J. Biol. Chem. (0021-9258) 2002 Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. taru-2002-3 Interaction of Alzheimer's beta -amyloid precursor family proteins with scaffold proteins of the JNK signaling cascade. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Taru H., Iijima K., Hase M., Kirino Y., Yagi Y., Suzuki T. Alzheimers - Interactions investigated in the context of Alzheimers disease J. Biol. Chem. (0021-9258) 2002 Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. taru-2002-5 Interaction of Alzheimer's beta -amyloid precursor family proteins with scaffold proteins of the JNK signaling cascade. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip monoclonal western monoclonal antibody western blot monoclonal western Taru H., Iijima K., Hase M., Kirino Y., Yagi Y., Suzuki T. Alzheimers - Interactions investigated in the context of Alzheimers disease J. Biol. Chem. (0021-9258) 2002 Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. taru-2002-4 Interaction of Alzheimer's beta -amyloid precursor family proteins with scaffold proteins of the JNK signaling cascade. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Taru H., Iijima K., Hase M., Kirino Y., Yagi Y., Suzuki T. Alzheimers - Interactions investigated in the context of Alzheimers disease J. Biol. Chem. (0021-9258) 2002 Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. a4_drome Amyloid-beta-like protein Appl CG7727 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE jip1_drome JNK-interacting protein 1 Protein eye developmental SP512 APP-like-interacting protein 1 Aplip1 SP512 CG1200 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADSEFEEFHRPIFEPHTIAGFGSGAGSKKNNPHAFYSLIPNDDLEDSHSSKSDGDGSDQEDGIGLVDHEPKMRQVEDDELGDGLKVTLSSDGSLDTNDSFNSHRHHPLNHQDAIGGFLGMDTSGLGGNSAPVTIGASTDLLAPNTAATRRRRKLPEIPKNKKSSILHLLGGSNFGSLADEFRNGGGGGIPPAVRSGQQRSFLSLKCGYLMDEDSSPDSERMQSLGDVDSGHSTAHSPNDFKSMSPQITSPVSQSPFPPPFGGVPFGQLEMLEATHRGLHKFVPRHHDEIELEIGDAIYVQKEAEDLWCEGVNLRTGRQGIFPSAYAVDLDYNEFDPTVQLVKKERYLLGYLGSVETLAHKGTGVVCQAVRKIVGEYGNSPTGQTCILEVSDQGLRMVDRSGPNQNKKDKKPCIDYFYSLKNVSFCAFHPRDHRFIGFITKHPTVQRFACHVFKGSESTRPVAEAVGRAFQRFYQKFIETAYPIEDIYIE jnk_drome Stress-activated protein kinase JNK bsk JNK CG5680 Protein basket protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKADCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYINVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR hep_drome Dual specificity mitogen-activated protein kinase kinase hemipterous hep hem MKK7 CG4353 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTIEFETIGSRLQSLEAKLQAQNESHDQIVLSGARGPVVSGSVPSARVPPLATSASAATSATHAPSLGAGSVSGSGISIAQRPAPPVPHATPFGSASASSSSSSASAFASAAPATGTFGGTYTPPTTRVSRATPTLPMLSSGPGGGLNRTRPVILPLPTPPHPPVSETDMKLKIIMEQTGKLNINGRQYPTDINDLKHLGDLGNGTSGNVVKMMHLSSNTIIAVKQMRRTGNAEENKRILMDLDVVLKSHDCKYIVKCLGCFVRDPDVWICMELMSMCFDKLLKLSKKPVPEQILGKVTVATVNALSYLKDKHGVIHRDVKPSNILIDERGNIKLCDFGISGRLVDSKANTRSAGCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATARSPYEGCNTDFEVLTKVLDSEPPCLPYGEGYNFSQQFRDFVIKCLTKNHQDRPKYPELLAQPFIRIYESAKVDVPNWFQSIKDNRLRANGDPTLQRATATGSAIGSGAGSLAGSGSGSAGGAVKYGRATTYAGQSPTNPQKTIKPTQIPSYQQQQSQFFMQSATQLPQTTTTTPTATTNCFGGSGNGNGRGNGSGGSGNGSGSSSSASPLSPPSAGIGDLNRLYRKSPFMQRKLSNGSHHPHYKYNDESPKKESMFSSIGQSILRNLTTSPFSQKKHNSTATTIPLPHNNQTLITDAATAAAAAATATTPPNIAATVLTTTPTTTPTWRLPTENSQAYDSCDSSSNATTTTLNLGLSSPSPSLPRKQFPTESPTLQLTSQQQQQPQRLQPGNQSPIVLQRFYHQQNQLREKEAAERYQQQRQQPPVGVTSTNPFHSNYVPPPPSTHSTSSQSSTQSTCSQIAINPASISPSSGSGTGNMAGLGIGSAPASGLGAAGHFGAGGTGEQLQYQPLPIAAEATGTSPTLQSRSPEQQSDYGGNGNMVASKLSKLYARRQLLGQSSSSGASNSSLDGCSREQHDAGWFNTLAGAMKRQFATYVKTQLNSTATSPVASSMDRDQEPVHPQPPAYRSVVNNGSGGKSYYYRTLSAASSSSNTSQSTSPTTEPLPGGGTSSFLRRYASSGPGGSISTPPSPHILAGLDRRHRSPDPPPRYNRGQSPLLLRKNLLELSGQPPGSPLLHRRYVSASPPLPPPRRGSESVPGSPQHFRTRIHYTPEPQRRIYRTIDQ jip1_mouse C-Jun-amino-terminal kinase-interacting protein 1 Mapk8ip1 Ib1 Jip1 Mapk8ip Prkm8ip Islet-brain-1 Mitogen-activated protein kinase 8-interacting protein 1 JNK MAP kinase scaffold protein 1 protein protein mouse Mus musculus Mouse MAERESGLGGGAASPPAASPFLGLHIASPPNFRLTHDISLEEFEDEDLSEITDECGISLQCKDTLSLRPPRAGLLSAGSSGSAGSRLQAEMLQMDLIDAAGDTPGAEDDEEEEDDELAAQRPGVGPPKAESNQDPAPRSQGQGPGTGSGDTYRPKRPTTLNLFPQVPRSQDTLNNNSLGKKHSWQDRVSRSSSPLKTGEQTPPHEHICLSDELPPQGSPVPTQDRGTSTDSPCRRSAATQMAPPSGPPATAPGGRGHSHRDRIHYQADVRLEATEEIYLTPVQRPPDPAEPTSTFMPPTESRMSVSSDPDPAAYSVTAGRPHPSISEEDEGFDCLSSPERAEPPGGGWRGSLGEPPPPPRASLSSDTSALSYDSVKYTLVVDEHAQLELVSLRPCFGDYSDESDSATVYDNCASASSPYESAIGEEYEEAPQPRPPTCLSEDSTPDEPDVHFSKKFLNVFMSGRSRSSSAESFGLFSCVINGEEHEQTHRAIFRFVPRHEDELELEVDDPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKNSDWIDQFRVKFLGSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEISVRGVKIGVKADDALEAKGNKCSHFFQLKNISFCGYHPKNNKYFGFITKHPADHRFACHVFVSEDSTKALAESVGRAFQQFYKQFVEYTCPTEDIYLE jip2_mouse C-Jun-amino-terminal kinase-interacting protein 2 Mapk8ip2 Ib2 Jip2 Islet-brain-2 Mitogen-activated protein kinase 8-interacting protein 2 JNK MAP kinase scaffold protein 2 protein protein mouse Mus musculus Mouse MADRAEMFSLSTFHSLSPPGCRPPQDISLEEFDDEDLSEITDDCGLGLSYDSDHCEKDSLSLGRSEQPHPICSFQDDFQEFEMIDDNEEEDDEEEEEEEEEEEDGDRQGKAGGGPGSQALAGDSLIPSPSLEESHKLRPTTLHLTTLGAQDSLNNNNGGFTSAPPSSWQETVLRSPAQEPLKELPAPLLPAEEERHEVQSLARPGCDCEGNQPPEPPASSGGASPSSDPGIEADLRSHSSGGHEGRRSSQELSSPGSDSEDAGGARLGRMISSISETELELSSDGGSSSGRSSHLTNSIEEASSPASEPEPEPEPLHEPPRRPAFLPVGQDDTNSEYESGSESEPDLSEDADSPWLLSNLVSRMISEGSSPIRCPGQCLSPAPRLPEEAASQANSVPQDCQDPEAGPHVELVDMDTLCGPPPPAPAAPRLGPAQPGPCLFLSNPTRDTITPLWATPGRTARPGRSCSAACSEEEEEDEEEDEEDEEDAEDSVVPPGSRTTGSTAPLDASLVYDAVKYTLVVDEHTQLELVSLRRCAGLGNDSEEDSSCEASEEEAGATLLGSDQVPEDASPDSPDLTFSKKFLNVFVNSTSRSSSTESFGLFSCVVNGEEREQTHRAVFRFIPRHPDELELDVDDPVLVEAEEDDFWFRGFNMRTGERGVFPAFYAHAVPGPAKDLLGSKRSPCWVDRFDVQFLGSVEVPCHQGNGILCAAMQKIATARKLTVHLRPPASCDLEISLRGVKLSLSGGGPEFQRCSHFFQMKNISFCGCHPRNSCYFGFITKHPLLSRFACHVFVSQESMRPVARSVGRAFLEYYQEHLAFACPTEDIYLE q9vx41_drome X11L Mint CG5678 Dmel\CG5675 Dmint Dmint1 MINT X11 X11-like X11Lalpha dX11 dX11L dX11Lalpha x11 x11Lalpha Dmel_CG5675 CG5675 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNSNSCSDSSNESMVNPPHIQMNFGQQAQQQQSHQHQQQQQQQERYVWEMRTRSPNVTPASTVLNSPDLNGELSYVPLQGLSPSGGGVQGSGSYGSNAGRDSGSYYYQRPQLHQIRLGRSPGSQFETRDVLPQGLASPGSPTDVGKADGQCLRDGEIVVFDDIDTNWMNKASSGQRPGSNEDVLKVTKMIGQLPIAEYEGSPRRFGSQPNTNTITMGGPRKRPPGFPQRVSPTTSVANNATAAAKASVGNLIDLVDHEEERSGTLREKTPTFDYLYEFSETRKVLEEFFKANPEDEKRYTDYTTESGDDVASSPQEYPATPMEQAYIGQRLARIPKDELYMVHRSPTKKPPSDQNPSTAYQEHNDIELYIDSNSRSSGDLADTELEANLRRHSRNFTLSPETTDYDSNCGDLDSLSNDINCPTDFGKLYTSMPVLEDGLSSGHASDTENNVSVVCDKQQSSQSQPVHEHNGNGERLENDYNVMSASLATLTTDASQSALISDFASLPMPPAPPPSSPPQIEIADASESMTMVPDADNPLDSHYGAVYAAALGGTHTEKSAGSGLGVTPVTAGGSLPSQAASEIQEAMKEIRSALQRAKTQPEKLKFCDEVLPTDPDSPVWVPRKGTTISTAQGAAADEEPDTDLETDRLLGQQRHDEQDFFADQSLADNNANEGTDIASNGHLNGTSDNNNPNPMNNSKPQTYSTATIRQGIGTSLTPNSPDICQIVGTTDISISSPEKLQFTKSPTGSIKSLKDSANSDKKAKSRNKEGLLEPKVLIEGVLFRARYLGSTQLVCEGQPTKSTRMMQAEEAVSRIKAPEGESQPSTEVDLFISTEKIMVLNTDLKEIMMDHALRTISYIADIGDLVVLMARRRFVPNSVVDPSITSPLGDVPTPGIGEEESPPKEPLSKHNRTPKMICHVFESDEAQFIAQSIGQAFQVAYMEFLKANGIENESLAKEMDYQEVLNSQEIFGDELEIFAKKELQKEVVVPKAKGEILGVVIVESGWGSMLPTVVIANLMSSGAAARCGQLNIGDQLIAINGMSLVGLPLSTCQSYIRNAKNQTAVKFTVVPCPPVVEVKILRPKALFQLGFSVQNGVICSLLRGGIAERGGVRVGHRIIEINNQSVVAVPHDTIVKLLSSSVGEILMKTMPTSMFRLLTGQETPIYI s6kl_drome Serine/threonine-protein kinase S6KL S6KL Bin4 Pk17E Protein kinase-like 17E S6 kinase-like protein CG7001 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGNSQPRNASRSRRTSQWPQGAAELGASASDHHYHQEELYQEQQQQQQQPQQEQPSSRRIQFQAHTNQPTQSHPPGDEEQPVQSQQQQLSTWSLGSLSGGRLNWSFSGARNSFRHRNKATRKSLTSLHGSRRGKTQWHRPLTNSIFNSHFKETSKNDLYRIDHLVAKGAFGVVFKVSSKSDISQCYALKVLKKSKLIEDNSVRQIKDEADIQKVCGHHPFIVKQIDLWQNRHNLHILSEYVPNGELFSKITHFSIDLVRLYIGEIALALDFLHNAGIIYRDAKPENILLTEQFHIKLTDFGLSKWLKLGANTRTMCGTFKYMAPEILCGEPYGHAVDWWALGVIACQMLTQKSPNIKRHLLRRRESVEPEDGLSNAPSIAQINGCLQDSDGDSEDFLPEEVQHLTHEGRDVLRKLLTIEPRQRIRSVMALQRIAIYKDYNLSSKQLLSLSPREIIARDGIRIYEDRHFDQLTNQCAIDAFLDF klc_drome Kinesin light chain Klc CG5433 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDMLRKNIENIELGLSEAQVMMALTSHLQNIEAEKHKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQEQDDACDKSRTDPVVELFPDEENEDRHNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLERTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKLSQLLTSNEKRRSKAIKEDLDFSEEKNAKP q9wv19_mouse Cytochrome P450 Cyp2g1 protein protein mouse Mus musculus Mouse MMLGGAFSIFMALCLSCLLILIAWKRTSKGGKLPPGPTPIPFLGNFLQVRTDATFQSFQKLQKKYGSVFTVYFGPRPVVVLCGHEAVKEALVDQADDFSGRGEMPTLEKNFQGYGLALSNGERWKILRRFSLTVLRNFGMGKRSIEERIQEEAGYLLEELHKVKGAPIDPTLYLSRTVSNVICSVVFGKRFDYQDQRFQSLMRMINESFVEMSKPWAQLYDMYWKVMQYFPGRHNYLYNLIEDLKDFIASRVKINEASFDPSNPRDFIDCFLIKMHQDKSDPHTEFNLKNLVLTTLNLFFAGTETVSSTLRYGFLLLLKYPEVEAKIHEEINQVIGTHRTPRVDDRAKMPYTDAVIHEIQRLTDIVPLGVPHNVTRDTHFRGYLLPKGTDVYPLFGSVLKDPKYFRYPDAFYPQHFLDEQGRFKKNDAFVVFSSGKRICVGEALARMELFLYFTSILQRFSLRSLVPPADIDIAHKISGFGNIPPVYELCFMAR appl-aplip1-1 GST pulldown detected by western blotting 14065 N/A 14070 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 14071 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false APLIP1 was expressed as GST fused protein djnk-hep-1 GST pull down of FLAG tagged hep by GST DJNK 14065 N/A 14072 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 14073 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false GST fused DJNK and FLAG tagged hep were used. djnk-jip1-1 GST pull down of mouse jip1 with GST tagged drome djnk 14065 N/A 14074 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 14072 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false GST tagged DJNK used. jip1-app-1 Interaction detected by GST-pull down assay. 14065 N/A 14070 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cytoplasmic region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 14074 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false jip2-app-1 Interaction detected in a GST-pull down assay. 14065 N/A 14070 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cytoplasmic region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 14075 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false x11l-app-1 Interaction detected in a GST pull down assay. 14065 N/A 14070 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cytoplasmic region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 14076 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false aplip1-appl-1 Interaction identified by Y2H. 14066 N/A 14070 unspecified role unspecified role bait bait cytoplasmic region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14071 unspecified role unspecified role prey prey physical association physical association false false false appl-x11l-1 Interaction between APPL and X11L identified by Y2H 14066 N/A 14076 unspecified role unspecified role prey prey N/A 14070 unspecified role unspecified role bait bait cytoplasmic region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain false physical association physical association false false false pk17e-appl-1 Interaction of Bin4 with APPL identified by Y2H. 14066 N/A 14070 unspecified role unspecified role bait bait cytoplasmic region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14077 unspecified role unspecified role prey prey physical association physical association false false false aplip1-appl-3 Aplip1 domain interacting with APPLcyt determined by Y2H. 14066 N/A 14071 unspecified role unspecified role prey prey c-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 14070 unspecified role unspecified role bait bait physical association physical association false false false appl-aplip1-3 Region of APPL interacting with APLIP1 determined by Y2H. 14066 N/A 14071 unspecified role unspecified role bait bait N/A 14070 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false appl-aplip1-2 coimmunoprecipitation of appl with flag-aplip1 14067 N/A 14070 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14071 unspecified role unspecified role prey prey physical association physical association false false false aplip1-appl-2 coimmunoprecipitation of flag aplip with anti appl antibodies 14067 N/A 14070 unspecified role unspecified role prey prey N/A 14071 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false aplip1-aplip1-2 transient coexpression and coimmunoprecipitation of tagged aplip1. 14067 N/A 14071 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14071 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false aplip1-klc-1 Interaction determined by coimmunoprecipitation. 14067 N/A 14078 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14071 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false aplip1-hep-1 transient coexpression and coprecipitation of aplip1 and hep. 14068 N/A 14073 unspecified role unspecified role bait bait N/A 14071 unspecified role unspecified role prey prey physical association physical association false false false FLAG tagged Aplip1 and HA tagged Hep app-jip1-1 interaction of FLAG tagged APP and mouse JIP1 detected by coimmunoprecipitation 14068 N/A 14079 unspecified role unspecified role prey prey N/A 14070 unspecified role unspecified role bait bait physical association physical association false false false APP is FLAG tagged. Mouse JIP1 is HA tagged. app-jip2-1 FLAG tagged APP was found to interact with mouse JIP2 by coimmunoprecipitation. 14068 N/A 14070 unspecified role unspecified role bait bait N/A 14075 unspecified role unspecified role prey prey physical association physical association false false false APP is FLAG tagged. Mouse JIP2 is HA tagged. app-x11l-1 interaction of APP with X11L protein was detected by coimmunoprecipitation. 14068 N/A 14070 unspecified role unspecified role bait bait N/A 14076 unspecified role unspecified role prey prey physical association physical association false false false APP was FLAG tagged. hep-aplip1-1 GST Pull down of hep with aplip1. 14069 N/A 14073 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 14071 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Flag tagged hep and APLIP1 is expressed as a GST-fusion protein. aplip1-aplip1-1 homodimerisation of aplip1. 14069 N/A 14071 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 Stoichiometry: 2.0 N/A 14071 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false GST-APLIP1 was used to pull down untagged APLIP1.