MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint zhang-2001-1 A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C. mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip polyclonal western polyclonal antibody western blot polyclonal western Zhang L., Wu SL., Rubin CS. J. Biol. Chem. (0021-9258) 2001 zhang-2001-3 A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhang L., Wu SL., Rubin CS. A highly sensitive Y2H complementation assay using the expression of beta-gal was used in combination with growth on his- plates. J. Biol. Chem. (0021-9258) 2001 zhang-2001-2 A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C. mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 imaging technique imaging technique microscopy immunostaining immunostaining Zhang L., Wu SL., Rubin CS. A combination of confocal immunofluorescence microscopy and mutagenesis were used to map a region of num-1 protein the mediates targeting/anchoring of tethered pkc-3. J. Biol. Chem. (0021-9258) 2001 zhang-2001-4 A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypical protein kinase C. mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip polyclonal western polyclonal antibody western blot polyclonal western Zhang L., Wu SL., Rubin CS. Coimmunoprecipitation assays were used to detect the formation of complexes between full-length pkc-3 and either wild-type or mutant num-1 isoform c proteins in intact cells. J. Biol. Chem. (0021-9258) 2001 q9xty6-1 Numb-related protein 1 cka1 num-1 cka-1 CKA1 Protein kinase C adapter 1 T03D8.1 protein protein caeel Caenorhabditis elegans MSASQGNVFTRGLSRISRRKKKTKSIQNSLVSEQQPSFDAAIVPMPIPNDKSSIFSKGMDRLRRSLRLPKKRRDRSHDRHLSPDVTGGSKTEQWQPDEGAVRTGTCCFNVKYLGSVEVYESRGMQVCEGALKSLKASRRKPVKAVLYVSGDGLRVVDQGNSRGLLVDQTIEKVSFCAPDRQTDKGFAYICRDGASRRWMCHGFLATKETGERLSHAVGCAFSICLEKKKRRDEETAQVNVQSAQESTSSTPPKDIFHPNWEDNTSEGTSTQNPSNSRSNLAYQSFRKHVSIEDRYLDPQSVIINEVPASNHMDEIRRISKPRPTGNPALFLRQGSLRAPPDMPSSSDQFKRNMSLRTVSNNPTERSPEKKSFGTQLYNEPIYEGDEDPLGLGITPPVVTKTSGSLSNNGLDGINLNWKSIPAPVHQMQQHNANGDFVAAWPQNTIEKPTVGPLDKLQKQFEDIKLISISSGENTPTTRSKADEWLDDVLRVSMSMSPTSPSSDPPSTSSYSVLPKSGPPPAHAPPPLPVRQAVSNGSPSIYQQQLQQANSTRNSPAGINWNSSPNPMKISQPPAKPVDPFDVQWSRLAVNNTH Isoform c is produced by alternative initiation at Met-45 of isoform a kpc3_caeel Protein kinase C-like 3 F09E5.1 pkc-3 aPKC Atypical protein kinase C-3 protein protein caeel Caenorhabditis elegans MSSPTSLEEDGDIKLKTRFQGQVVVLYARPPLILDDFFALLKDACKQHKKQDITVKWIDEDGDPISIDSQMELDEAVRCLNSSQEAELNIHVFVGKPELPGLPCQGEDKTVYRRGARRWKKIYLYNGHRFQAKRLNRRIQCFICHDYIWGIGRQGFRCVDCRLCVHKKCHRHVRTHCGQALQGPNIIPMAPASGSLKGARSNTSSSTTRSGGGIDNGAFHEHEIESPGSTSHDASRAMNGNGSSKWAVSLNDFRLLTVIGRGSYAKVVQAEHVSTRQIYAIKIIKKEMFNEDEDIDWVQTEKSVFEAASNYPFLVGLHSCFQTESRLFFVIEFVPGGDLMFHMQQQRKLPEEHARFYSGEIILALHFLHSRGIIYRDLKLDNVLIDAEGHIKLTDYGMCKENIKDGDLTSTFCGTPNYIAPEILRGDEYGFSVDWWALGVLMFEMMAGRSPFDIVGMQNSEENTEDYLFQIILERQIRIPRSLSVRASGILKGFLNKDPTERLGCKLDINEGLRDMKEHQFFRGFIDWEALEQKAVAPPYHPAVESDRDLTHFDHQFTDELPQLSPDNPDVIARIDQSEFDGFEYVNPLQMSREDSV numb1_caeel Numb-related protein 1 Protein kinase C adapter 1 CKA1 num-1 cka-1 T03D8.1 protein protein caeel Caenorhabditis elegans MSASQGNVFTRGLSRISRRKKKTKSIQNSLVSEQQPSFDAAIVPMPIPNDKSSIFSKGMDRLRRSLRLPKKRRDRSHDRHLSPDVTGGSKTEQWQPDEGAVRTGTCCFNVKYLGSVEVYESRGMQVCEGALKSLKASRRKPVKAVLYVSGDGLRVVDQGNSRGLLVDQTIEKVSFCAPDRQTDKGFAYICRDGASRRWMCHGFLATKETGERLSHAVGCAFSICLEKKKRRDEETAQVNVQSAQESTSSTPPKDIFHPNWEDNTSEGTSTQNPSNSRSNLAYQSFRKHVSIEDRYLDPQSVIINEVPASNHMDEIRRISKPRPTGNPALFLRQGSLRAPPDMPSSSDQFKRNMSLRTVSNNPTERSPEKKSFGTQLYNEPIYEGDEDPLGLGITPPVVTKTSGSLSNNGLDGINLNWKSIPAPVHQMQQHNANGDFVAAWPQNTIEKPTVGPLDKLQKQFEDIKLISISSGENTPTTRSKADEWLDDVLRVSMSMSPTSPSSDPPSTSSYSVLPKSGPPPAHAPPPLPVRQAVSNGSPSIYQQQLQQANSTRNSPAGINWNSSPNPMKISQPPAKPVDPFDVQWSRLAVNNTH pkc3-num1-1 Coimmunoprecipitation of pkc-3 and num-1 detected by Western blot. 7350 N/A 7354 unspecified role unspecified role prey prey mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 N/A 7355 unspecified role unspecified role bait bait mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 physical association physical association false false false Pkc-3 interacts with both isoform a and isoform c of num-1. pkc3-num1-2 Coimmunoprecipitation of pkc-3 and num-1 detected by Western blot. 7350 N/A 7355 unspecified role unspecified role prey prey mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 N/A 7356 unspecified role unspecified role bait bait mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 physical association physical association false false false Num-1 interacts with pkc-3. pkc3-num1-4 A sensitive Y2H complementation assay (beta-galactosidase) in combination with deletion mutagenesis. 7351 N/A 7355 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 7356 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false The smallest num-1 fragment retaining avid pkc-3 binding activity included AAs 88-235 which corresponds to an intact PTB (phosphotyrosine binding) domain (AA 90-231) pkc3-num1-5 A sensitive Y2H complementation assay (beta-galactosidase) in combination with mutational mutagenesis. 7351 N/A 7356 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9XTY6:p.Phe221Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 7355 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7373 7371 physical association physical association false false false The Phe-221 is embedded within the alpha3-helix of num-1 and is essential for correct binding with pkc-3. pkc3-num1-3 Immunofluorescence used to detect targeting/anchoring of tethered pkc-3. 7352 N/A 7355 unspecified role unspecified role neutral component neutral component mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 N/A 7356 unspecified role unspecified role neutral component neutral component region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 colocalization colocalization false false false Num-1 interacts with pkc-3 and targets the pkc-3/num-1 complex to the cell periphery. pkc3-num1-6 Coimmunoprecipitation of pkc-3 and num-1 mutants detected by Western blot. 7353 N/A 7355 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 N/A 7354 unspecified role unspecified role bait bait Q9XTY6-1:p.Phe221Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9XTY6-1:p.Phe175Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false mesau-av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line av12-644 adenovirus type-12-transformed Syrian hamster subcutaneous tumor cell line ATCC# CRL9595 (MCB3901) (fibroblasts) http://www.lgcpromochem.com/atcc ATCC number CRL 9595 physical association physical association false false false Pkc-3 is unable to interact with the num-1 isoform c mutants phe175leu or phe221leu but can interact with the mutant ser144ala . Phe175 and ser174 are included in the beta5-strand and phe221 is embedded within the alpha3-helix. Thus, large hydrophobic side chains with aromatic character provide local apolar surfaces that govern (in part) the affinity of num-1 for pkc-3 and/or stability of cka-1/pkc-3 complexes.