HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html hsieh-1998-3 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-6 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-1 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. human-liver Homo sapiens liver liver liver 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-2 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-4 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-5 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast confocal microscopy confocal microscopy immunostaining immunostaining Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation hsieh-1998-7 Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Hsieh T.Y., Matsumoto M., Chou H.C., Schneider R., Hwang S.B., Lee A.S., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu J. Biol. Chem. (0021-9258) 1998 imex curation Only protein-protein interactions imex curation p29846-pro_0000037666 Core protein precursor protein protein hcvtw Hepatitis C virus genotype 1b (isolate Taiwan) (HCV) STNGKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTWERSQPRGRRQPIPKARQPEGRAWAQPGYPWPLYGNEGLGWAGWLVSPRGSRPNWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGVARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPASA 2 191 hnrpk_human Heterogeneous nuclear ribonucleoprotein K HNRNPK HNRPK Transformation up-regulated nuclear protein protein protein human Homo sapiens Human METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF p29846_pro-hnrnpk-1 1457389 n/a Core 1457396 unspecified role unspecified role bait bait 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hnRNP K 1457397 unspecified role unspecified role prey prey gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3B hnrnpk-p29846_pro-2 1457389 n/a Core 1457396 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a hnRNP K 1457397 unspecified role unspecified role bait bait 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3D hnrnpk-p29846_pro-4 1457390 n/a Core 1457396 unspecified role unspecified role unspecified role unspecified role gal4 bd gal4 dna bd gal4 dna binding domain gal4 dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a hnRNP K 1457397 unspecified role unspecified role unspecified role unspecified role gal4 ad gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1457413 1457410 physical association physical association false false false Fig. 7 and 8 hnrnpk-p29846_pro-3 1457390 n/a hnRNP K 1457397 unspecified role unspecified role unspecified role unspecified role gal4 ad gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a Core 1457396 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gal4 bd gal4 dna bd gal4 dna binding domain gal4 dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1457419 1457417 physical association physical association false false false Fig. 8 core-hnrnp k-2 1457391 n/a hnRNP K 1457397 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a Core 1457396 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1457425 1457423 physical association physical association false false false Fig. 1 core-hnrnp k-1 1457392 n/a Core 1457396 unspecified role unspecified role prey prey 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hnRNP K 1457397 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2B core-hnrnp k-3 1457393 n/a Core 1457396 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a hnRNP K 1457397 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5 hnrnpk-p29846_pro-1 1457394 n/a Core 1457396 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hnRNP K 1457397 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 6 core-hnrnpk-2 1457395 n/a Core 1457396 unspecified role unspecified role bait bait 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hnRNP K 1457397 unspecified role unspecified role prey prey 35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 4