IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yang-2020-15 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Yang M., He S., Chen X., Huang Z., Zhou Z., Zhou Z., Chen Q., Chen S., Kang S. Virus - Publications including interactions involving viral proteins chenshd5@mail.sysu.edu.cn,kangss@mail2.sysu.edu.cn Frontiers in chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus yang-2020-14 ecoli Escherichia coli (strain K12) bn-page blue native page bn-page weight identificat confirmation by molecular weight weight identificat Yang M., He S., Chen X., Huang Z., Zhou Z., Zhou Z., Chen Q., Chen S., Kang S. Virus - Publications including interactions involving viral proteins Proteins underwent size-exclusion chromatography prior to BN-Page. chenshd5@mail.sysu.edu.cn,kangss@mail2.sysu.edu.cn Frontiers in chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus yang-2020-13 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yang M., He S., Chen X., Huang Z., Zhou Z., Zhou Z., Chen Q., Chen S., Kang S. Virus - Publications including interactions involving viral proteins chenshd5@mail.sysu.edu.cn,kangss@mail2.sysu.edu.cn Frontiers in chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus yang-2020-16 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yang M., He S., Chen X., Huang Z., Zhou Z., Zhou Z., Chen Q., Chen S., Kang S. Virus - Publications including interactions involving viral proteins chenshd5@mail.sysu.edu.cn,kangss@mail2.sysu.edu.cn Frontiers in chemistry 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus trs-1 rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) ACGAAC ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA trs-2 rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) AAACGAAC trs-3 rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) AAACGAACUU duplex-trs-1 ds rna ds rna double stranded ribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) ACGAAC duplex-trs-2 ds rna ds rna double stranded ribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AAACGAAC duplex trs-3 ds rna ds rna double stranded ribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AAACGAACUU imperfectly paired duplex trs-1_chain_b imperfectly paired duplex trs-1_chain_b rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) GUUCGUUU imperfectly paired duplex trs-1_chain_a rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) ACGAAC imperfectly paired duplex trs-2_chain_b-1 rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) AAGUUCGUUU imperfectly paired duplex trs-2_chain_a rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) AAACGAAC trs_1-n-2 3975389 N/A 3975393 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false LKR+CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4C SARS-CoV-2 N-CTD showed a very weak binding affinity, however addition of either IDR to the N-CTD sequence increased binding affinity. trs_2-n-1 3975389 N/A 3975395 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false LKR+CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4D As the length of the TRS increased, the binding affinities were enhanced. trs_2-n-2 3975389 N/A 3975396 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false LKR+CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4E As the length of the TRS increased, the binding affinities were enhanced. trs_3-n-1 3975389 N/A 3975397 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3975389 5B duplex_trs_2-n-1 3975389 N/A 3975398 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3975389 5C duplex_trs_2-n-2 3975389 N/A 3975399 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3975389 5D duplex trs_3-n-1 3975389 N/A 3975400 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975401 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3975389 5E imperfectly paired duplex trs_1_chain_a-n-1 3975389 N/A 3975402 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975403 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3975394 unspecified role unspecified role prey prey purified purified CTD+IDR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3975389 5F The methods section states:- "Unpaired Duplex TRS2 was obtained by an annealing process of RNA2 with RNA4." However the sequences given in figure 5A correspond to RNA2 and RNA6. n-77 3975390 N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false 2E n-78 3975390 N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC9:p.Ser327Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false Cys327 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC9:p.Ser327Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false Cys327 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false 3975465 3975461 direct interaction direct interaction false false false 2E The mutants are in a higher polymerization state than the WT dimer. n-n-49 3975390 N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC9:p.[Gln289Cys;Arg293Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Cys289 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false Cys293 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0DTC9:p.[Gln289Cys;Arg293Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Cys289 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false Cys293 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 2E The mutants are in a higher polymerization state than the WT dimer. Figure 2E gives an R294 C mutation;however there is no Arginine residue at position 294 and figure 2CII refers to R293C, so the use of "R294C" is probably a typo. n-76 3975391 N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 n-79 3975392 N/A 3975394 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. figure 1B