IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rogstam-2020-1 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rogstam A., Nyblom M., Christensen S., Sele C., Talibov VO., Lindvall T., Rasmussen AA., André I., Fisher Z., Knecht W., Kozielski F. Virus - Publications including interactions involving viral proteins f.kozielski@ucl.ac.uk International journal of molecular sciences 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus rogstam-2020-2 ecoli Escherichia coli (strain K12) solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Rogstam A., Nyblom M., Christensen S., Sele C., Talibov VO., Lindvall T., Rasmussen AA., André I., Fisher Z., Knecht W., Kozielski F. Virus - Publications including interactions involving viral proteins f.kozielski@ucl.ac.uk International journal of molecular sciences 2020 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus p0dtd1-pro_0000449628 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ 4254 4392 rep-142 3940133 N/A 3940135 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false 2A, Supp. Figure 2 One major and one minor peak were observed. These peaks most likely correspond to monomeric and dimeric nsp10. The results showed that after long-term storage at high concentration, aggregates form, and the amount of dimers increases substantially. rep-143 3940134 N/A 3940135 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false 2 B/C, Table S3 The predominant species is consistent with the theoretical molecular weight of monomeric nsp10. The second-most-populous species is consistent with dimeric nsp10. There was no evidence of oligomeric states higher than dimers in freshly prepared nsp10.