IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zeng-2018-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Journal of virology 2018 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zeng-2018-3 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Journal of virology 2018 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zeng-2018-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Journal of virology 2018 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus p0c6y4-pro_0000037377 Non-structural protein 9 rep ORF1ab polyprotein 1a-1b protein protein pedv Porcine epidemic diarrhea virus (strain CV777) NNEIIPGKLKQRSIKAEGDGIVGEGKALYNNEGGRTFMYAFISDKPDLRVVKWEFDGGCNTIELEPPRKFLVDSPNGAQIKYLYFVRNLNTLRRGAVLGYIGATVRLQ 3858 3965 x2g6c4_9nido 3C-like proteinase orf1ab Growth factor-like peptide Helicase NendoU Non-structural protein 10 Non-structural protein 2 Non-structural protein 3 Non-structural protein 4 Non-structural protein 6 Non-structural protein 7 Non-structural protein 8 Non-structural protein 9 ORF1ab polyprotein RNA-directed RNA polymerase Replicase polyprotein 1ab Uridylate-specific endoribonuclease Papain-like proteinase nsp5 p195 protein protein Porcine coronavirus HKU15 Porcine coronavirus HKU15 MAKNKSKRDAIALPENVPPPLQLFIHVAAAEEGHPKVTTYLGNYNLYATKAPPGVQVLSAKTSLTDFENVFGAQPTLRSIRNLVCEARSAEWTISKNAFALKATQLDYSDAVLRAMIRFCPPKVSTLAAFALFGRLVKIEDKELAELARDTALELAYTAKIGTSLADTRSVSLIHKDAYLTLSNEVVGVTFTAALMAKATTVNGAMQYSNFYLYPRATIKVTDGKAEAIATKPLSAATKGKQITEDVNLLPDYQQLLVDQVTGTEVKVGALTYVKTTDSPPLYFPKVKGGVIGIALKQQGTAAKKLNVVFHAQPDDVLLAFIQLQQFLNRTSDSSVEITDCQSYEVSPTVTVKIGPSKPGDVIVATDEEYLKCFETPEVGRLYKVFQTQSWAIIERAFSSLKIRVSKALSAFISFLQNLADNFTAISGVVTALIRELQDLTLDVATRITNIQFVYRAGKLIVDTTSVIAKLFQPFCDFISPFLRKVAGFAIYTVGNRMLMFTSTGTFLLTKATTKILNKAKYIFDVEPEYPVDVTTSKVVVHEALQQTDTKPTRALEAVDVVVGNTVLQMATDGTAFYPSDGTHASLPGFKAGSDELFISFNCDLFDDETNAQINETLAAYELNQLVAPGDSTPRQIATLVVDTLADAITDHFPEKTIDLPEDYQVFSDHDDLPLAQYHIPDHLSLYIQAMEGEDDSGDEICIEDDDYDCPQADEDTEGVIPQQWELPDVDKFLLKIQERKTSSDEVLSVDVYPKPEPVGNVGIDDSASEKKPNGDSVPDPEVHPTLESVDVERPTETANQAVEDKPSDTTFVVDEEQLQESTPEHELRSYEGEFDSDDEIIIPIVPVTPADLKPQTITIKEYFKSEKLETINEGSTESVTQSDDSFDESFVDAESDDPQDPAVYDDTTIITDSTDVGDEPETTLATIVNTPLTLDNNLPPEAIKQPSPTKVELVVGELASIKFDNSVLVNPANAQLTNGGGAARAIAKLAGPKYQEYCNSVAPISGPLTTDSFDAKKFGVACILHVVPPKGSDPNVQELLYQAYKSILTEPAHYVIPILGAGIFGCNPVHSLDAFRKACPSDIGRVTLVTMNKNHLQVWDALNRTIVRTTTDYDQVTTKALTPQGVLEANLFDGEDFVQEPKPGQIYLEVTEEVQNQAKELDLNLQQYCVYLKTCHHKWVVSRTNGLMHLKQKDNNCFVSAGVNLFQNTAYQLRPAIDALYREYLNGNPNRFVAWIYASTNRRVGEMGCPQQVISLLVSNSDAAFSATTACCNTYFNHTGVISVAREYDPIQPKVYCMKCDVWTPFTPQSGKGAVAIGISADEPTGPAIKFAAAHCWYTNGKKTVNGYDTKANVVATYHRFDVPKPQLVEDVVALPTKNDFEVLNVEELPQDSVLHLDPPPVQALQPKANQHIEILENPDYLDILDLWIRKPKFILVKSWSVLGRALCKAGKVVFVSASLLTRFYNYLVEIGALDSTIRLSVDLTCKFVRTVLPSSNTVHKTCLGLYYSAQTLFVSLAPFLMLPAVVSLLNSGYTIGTYLYAKTGWPCNYNATQHFDYNSYCAGDLVCQACFDGQDSLHLYPHLRVNQQPLQTTDYTVYALSLILLLANMTLVMGTLIVTFFVNFYGVQIPFYGTLLIDYQSALVITFSVYYFYKVMKFFRHLTHGCKIPTCVVCAKLRTPPTITVETVVQGRKYPSVIETNGGFTICKEHNFYCKDCSLQTPGTFIPTEAIESLSRATRLSVKPTAPAFLLARDVECQTDVVVARAMHNQNAHVCISKYSDIRTVDQLLKPTPLFSYTPDVIIAADFDNRGSLKTAKELAVVLSMDLKRTIIIIDQAYSRPIDNYQEVASRIEKYYPVAKITPTGDIFTDIKQATNGQASDSAINAAVLAVQRGLDFTIDNPNNILPHYAFDFSTLNAEDQSTILESGCAKGNLKGTNVGVVLSASLVTRLSQQAIRVIANAASRNGVTCAVTPSTLVMRGNIATQPLTRIKAGAPPMRQKILCVILALAIVYFAAMAFGFLASQITLNTVPTIKSDIRASTFYVVRDGVLDTVRSNDKCFANKFLAFDSFIQAPYTNSPDCPVVVGVVDVTTHSIPGIPAGVIHRDGLILNIYEQSLYETHQRQSMVRDALSLKTANLFNLGKRVVVGYTQHEVVVGTSYFNSPALFNAKCTFLQYQDTRQLYCYDTVPTEHKLYSDVLPHVEYKAIDINGDLVPFKIPEQIMFYPHIVRYTSNSYCRMGHCFNTNPGICISFTDEFPYSENVKPGVYCADTSLQLFSNLVLGTVSGIHIFTSTAALLGSTIVIILCVVAVLAVQRFFKEYTTFVMYTCGLALVNIVGIALMYKCLVFAIFYYAIYLYFVLTFPSFKRNVALFYFAVVIVPHVSNMQLLALIVCSIIYFLYTYVHTVAKTAGKFSSFLDAAKATFVIDNEKYVLLKDLAGAEFDQYLASYNKYKYFSGTASDKDYDKVCMAFLAKALSSFREGGGSQLYTPPKFAVVQSLKTKLQAGIKILLHPSGVVERCMVSVVYNGSALNGIWLKNVVYCPRHVIGKFRGDQWTHMVSIADCRDFIVKCPIQGIQLNVQSVKMVGALLQLTVHTNNTATPDYKFERLQPGSSMTIACAYDGIVRHVYHVVLQLNNLIYASFLNGACGSVGYTLKGKTLYLHYMHHIEFNNKTHSGTDLEGNFYGPYVDEEVIQQQTAFQYYTDNVVAQLYAHLLTVDARPKWLAQSQISIEDFNSWAANNSFANFPCEQTNMSYIMGLSQTARVPVERILNTIIQLTTNRDGACIMGSYDFECDWTPEMVYNQAPISLQSGVVKKTCTWFFHFLFMAITMLLAAMHVFPVHLYPIVLPCFTVVAFLLTLTIKHTVVFTTTYLLPSLLMMVVNANTFWIPNTFLRTCYETIFGSPIAQRLYGYTVALYMLIYAGLAINYTLKTLRYRATSFLSFCMQWFQYGYVAHIAYKLLNKPWTESLLFTAFTMLTSHPLLAALSWWLAGRVTLPIIMPDLAIRVLAYNVIGYVICVRFGLMWLANRFTTVPMGTYQYMVSVEQLKYMMAVKMSPPRNAFEVLIANIRLLGLGGNRNIAVSTVQNKILDAKATAVVVANLLEKAGVTNKHAICKKIVKLHNDTLKATTYEEVEVALVKLLSHIIEFLPTDQVDAYLADAANAQHVNTYFDNLLENKAVVQAVADININLDSYRIYKEADAIYKRSVEMNESPQEQKKKLKAVNIAKAEWEREAASQRKLEKLADAAMKSMYLAERAEDRRIKLTSGLTAMLYHMLRRLDSDRVKALFECAKAQILPIHAVVGISNDNLKVIFNDKDSYSHYVEGNTLIHKGVRYTIVKKLSLDNAPIEGVPEEFPVVVETVREGVPQLQNNELCLRNVFTAQNTAQDFNGNESTVKSFYVTRTGKKILVAITSTKDNLKTVTCLTETGKTVLNLDPPMRFAHTVGGKQSVVYLYFIQNISSLNRGMVIGHISETTILQASGTQIEYQQNASLLTYLAFAVDPKTAYLKHLADGGSPIQGCIQMIATMGPGFAVTTKPQPNEHQYSYGGASICLYCRAHIPHPGVDGRCPYKGRFVHIDKDKEPVSFALTHEPCSSCQRWVNYDCTCGSSLQNSAYLNRVTGSSDARLEPLQPGTQPDAVKRAFHVHNDTTSGIFLSTKSNCARFKTTRSALPLPNKGEVELYFVTKQCAAKVFEIEEECYNALSTELYTTDDTFGVLAKTEFFKFDKIPNVNRQYLTKYTLLDLAYALRHLSTSKDVIQEILITMCGTPEDWFGENWFDPIENPSFYKEFHKLGDILNRCVLNANKFASACIDAGLVGILTPDNQDLLGQIYDFGDFIITQPGNGCVDLASYYSYLMPIMSMTHMLKCECMDSDGNPLEYDGFQYDFTDFKLGLFEKYFKYWDRPYHPNTVECPDDRCVLHCANFNVLFAMCIPNTAFGNLCSRATVDGHLVVQTVGVHLKELGIVLNQDVTTHMANINLNTLLRLVGDPTTIASVSDKCVDLRTPCQTLATMSSGIAKQSVKPGHFNQHFYKHLLDSNLLDQLGIDIRHFYYMQDGEAAITDYSYYRYNTPTMVDIKMFLFCLEVADKYLEPYEGGCINAQSVVVSNLDKSAGYPFNKLGKARNYYDMTHAEQNQLFEYTKRNVLPTLTQMNLKYAISAKDRARTVAGVSIISTMTNRQYHQKMLKSISLARNQTIVIGTTKFYGGWDNMLRRLMCNINNPILVGWDYPKCDRSMPNMLRIAASCLLARKHTCCNQSQRFYRLANECCQVLSEVVVSGNNLYVKPGGTSSGDATTAYANSVFNILQVVSANVATFLSTSTTTHLNKDIADLHRSLYEDIYRGDSNDITVINRFYQHLQSYFGLMILSDDGVACIDSAVAKAGAVADLDGFRDILFYQNNVYMADSKCWTETDMNVGPHEFCSQHTVLAEHDGKPYYLPYPDVSRILGACIFVDDVNKADPVQNLERYISLAIDAYPLTKVDPIKGKVFYLLLDYIRVLAQELQDGILDAFQSLTDMSYVNNFMNEAFYAQMYEQSPTLQASGVCVVCNSPTILRCGDCIRRPLLCCVCAYQHVTQTTHKRIIAINNYICSVENCNEDNVEKLFISGTAIYCENHKPTLCIPIVANGSVFGIYRHTARGSDDIDLFNELATSNYDTIEPYQKANRAPLSLMLFAAETIKALEESIKKSYATATVKDVYDQRFIKLLWEQGKKPPPITKNHIFTGYHFNKNGKTQVGDYILAKTDGSDTYTYRGTSTYKLQTGDVLVLMAHVVTPLSAPPVLTQTTYVRKSLLPDSVGASYYVQHFKSYNEIAMQRVTTVLGPPGTGKSTFAIGLAKYFPSARICYTASSHAAIDALCEKAFKTIPVGQCSRIVPTRTTVECFQEFVVNNTTAQYIFSTINALPDIKCDIVVVDEVSMLTNYELSSVNARLVYNHIVYVGDPYQLPSPRTMLTSGQLSPADYNVVTDIMVHAGADVMLDMCYRCPREIVETVSKLVYDNKLKAAKPNSRQCYKTIVNFGPGDVAHEGQSAYNEAQLRFALAFRQQKRWDNVTFISPYNAMNVKASLAGFSTQTVDSSQGSEYDYVIFCVTTDSAHALNMARLNVALTRAKIGILVVFRQANELYNSLQFESIDSQLQSSAEKNLTPLFKRCGYEYNGVHPAHALTWHDCGAEYRCEEPLAKLVGVADGTLISYKTLVSTLGFLPSLKIDAYHNMFLTRDACRTYVQSWIGIDVEAAHAIKPNTGTNLPLQIGFSTGKNFSVTPEGIWVNEHGSCTEPVPAKIPPGEQFRHLKKDMRQARPWKVVRREIATHIAEVAPHTDYICFVTWAHQLELATMRYFVKLGMEEKCFCGRRACFTNGTEFACKAHHSLTIPQCDYVYNPFLIDVATWGFSGRLSTNHDAVCTYHANAHVASADAIMTVCLAIHELFSTVDWNFEFPVTAEQSQLNKACRLVQANYLNILLTTTKATVVHDIGNPKGIPIVRKPGVKYHFYDQAPIVKHVQKLKYKPEMEARFTDGLTMFWNCNVDTYPANALVCRYDTHRQKHLIGPNGSALYVNKHAFLTPEMHTYATHKLNLAPLIYYSTTDCSSEQPIVVTYRDCVTRCNTGKTLCPNHALEYQEFINAYNLMARHGFNVYIPRNVNVYNCWLTFTNLQNLENLAYNCYYKNCNAHVDGQLDVVINNNAVYAKVDNNLVKLFDNRTNLPVSVAFEHYTNRHTRSLPTTQLLSGLGVTATRNFTVWFDNDTIFQYTINVSTYTDIDPSTHVVLCDDRYGTDWSQFNQLPNAVFLTKTKVKKTEPFVCTALTLNGVAIDGEELYIYVRYNNQLTTFATTCTQGRNVEQFIPKTPMERDFLEMSQQSFIQQHQLQELGVEHIIYGDDSSPVIGGTHTLISLVKNKFEHQLVNHVYNPVQNCVVTSPNASSKNVCTVLDVLLDDYIDIIRQAHASYTSKSKVFTVSIDNQQIRFMLWHDEQVKTCYPILQSLTNGYQMPSVYKTLVTDLQPADIPNYHSYTPRVPGVVKNVIKYRQLFNYIVKKDRLAVPHNMTVLHLGAASALGTAPGSSVIKQMFPEGTVLIDLDIREFTSDANQIIVTDYRTYIPPHHVDVIFSDLYCCDDIHFFDNLIRIVKERLALGGSIFVKITEHSFSPELYSLAGWFDDYQLFCTAVNASSSEAFLCCFNYLGLAKENINGFNLHASYIQWRNEIALTPTYSPLADNPATACKLKATPIISARELEKKPILRYLVASGRLLVRPPECRELY nsp9-dimer-1 3710622 N/A 3710625 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 rep-76 3710622 N/A 3710626 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false p.Asn3378_Arg3384del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 rep-77 3710622 N/A 3710625 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0C6Y4-PRO_0000037377:p.Cys59Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table 1 orf1ab-5 3710623 N/A 3710626 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false p.Asn3378_Arg3384del delta7N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false X2G6C4:p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla] L4A/L6A/R7A/N8A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false X2G6C4:p.Arg3384Ala R7A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false X2G6C4:p.Gly3473Glu G96E mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false X2G6C4:p.Gly3477Glu G100E mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2F rep-78 3710623 N/A 3710625 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0C6Y4-PRO_0000037377:p.Cys59Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5E orf1ab-4 3710624 N/A 3710626 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false p.Asn3378_Arg3384del delta7N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false X2G6C4:p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2 H, 2E, 2G