IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chu-2018-2 mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus bCoV-HKU9 spike glycoprotein Uniprot accession was taken from back referenced 27696819 The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation chu-2018-5 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation chu-2018-6 human-beas_2b Human bronchial epithelial cell line BEAS-2B beas_2b Human bronchial epithelial cell line BEAS-2B http://cancerres.aacrjournals.org/cgi/content/abstract/48/7/1904?ijkey=330aa4101c1c77d6fbc2b1fc3d60b4ead4af2511&keytype2=tf_ipsecsha anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation chu-2018-1 human-beas_2b Human bronchial epithelial cell line BEAS-2B beas_2b Human bronchial epithelial cell line BEAS-2B http://cancerres.aacrjournals.org/cgi/content/abstract/48/7/1904?ijkey=330aa4101c1c77d6fbc2b1fc3d60b4ead4af2511&keytype2=tf_ipsecsha anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation chu-2018-3 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation chu-2018-4 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY., Chu H., Chan CM., Zhang X., Wang Y., Yuan S., Zhou J., Au-Yeung RK., Sze KH., Yang D., Shuai H., Hou Y., Li C., Zhao X., Poon VK., Leung SP., Yeung ML., Yan J., Lu G., Jin DY., Gao GF., Chan JF., Yuen KY. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus The range of S1 in spike glycoprotein and its tag features were annotated from back referenced 27489282 The viral strain was provided by Ron Fouchier, which is Human betacoronavirus 2c EMC/2012 isolate, with GenBank accession number, JX869059 (from PMID 23075143); S glycoprotein accession was chosen based on AFS88936.1 id provided in the GenBank submission kyyuen@hku.hk,jfwchan@hku.hk The Journal of biological chemistry 2018 Only protein-protein interactions imex curation imex curation bip_human Endoplasmic reticulum chaperone BiP HSPA5 GRP78 Immunoglobulin heavy chain-binding protein 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein human Homo sapiens Human MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL k0brg7_mers Spike glycoprotein E2 Peplomer protein S protein protein cv2ce Human betacoronavirus 2c EMC/2012 MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFQKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH a3exg6-pro_0000291369 Spike protein S1 S E2 Peplomer protein 2 protein protein BtCoV-2 Bat coronavirus HKU9 BtCoV/HKU9 AAASRPECFNPRFTLTPLNHTLNYTSIKAKVSNVLLPDPYIAYSGQTLRQNLFMADMSNTILYPVTPPANGANGGFIYNTSIIPVSAGLFVNTWMYRQPASSRAYCQEPFGVAFGDTFENDRIAILIMAPDNLGSWSAVAPRNQTNIYLLVCSNATLCINPGFNRWGPAGSFIAPDALVDHSNSCFVNNTFSVNISTSRISLAFLFKDGDLLIYHSGWLPTSNFEHGFSRGSHPMTYFMSLPVGGNLPRAQFFQSIVRSNAIDKGDGMCTNFDVNLHVAHLINRDLLVSYFNNGSVANAADCADSAAEELYCVTGSFDPPTGVYPLSRYRAQVAGFVRVTQRGSYCTPPYSVLQDPPQPVVWRRYMLYDCVFDFTVVVDSLPTHQLQCYGVSPRRLASMCYGSVTLDVMRINETHLNNLFNRVPDTFSLYNYALPDNFYGCLHAFYLNSTAPYAVANRFPIKPGGRQSNSAFIDTVINAAHYSPFSYVYGLAVITLKPAAGSKLVCPVANDTVVITDRCVQYNLYGYTGTGVLSKNTSLVIPDGKVFTASSTGTIIGVSINSTTYSIMPCVTVPVSVGYHPNFERALLFNGLSCSQRSRAVTEPVSVLWSASATAQDAFDTPSGCVVNVELRNTTIVNTCAMPIGNSLCFINGSIATANADSLPR 12 676 hspa5-s-2 3679490 N/A 3679496 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3679497 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed electroporation electroporation s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2a s-hspa5-1 3679490 N/A 3679496 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3679497 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed electroporation electroporation s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2b hspa5-s-6 3679490 N/A 3679496 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3679498 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed electroporation electroporation c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 8a hspa5-s-3 3679491 N/A 3679496 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role prey prey infection infection physical association physical association false false false 2e hspa5-s-4 3679491 N/A 3679496 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role bait bait infection infection physical association physical association false false false 2e hspa5-s-5 3679492 N/A 3679496 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role prey prey infection infection physical association physical association false false false 2e s-hspa5-4 3679492 N/A 3679497 unspecified role unspecified role bait bait infection infection N/A 3679496 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 2e hspa5-s-1 3679493 N/A 3679496 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1c-e s-hspa5-2 3679494 N/A 3679496 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2c s-hspa5-3 3679495 N/A 3679496 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3679497 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed s1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2d