HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html salinas-2014-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu Journal of virology 2014 imex curation Only protein-protein interactions imex curation salinas-2014-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu Journal of virology 2014 imex curation Only protein-protein interactions imex curation salinas-2014-1 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins Mass spectrometry and SILAC-based interaction screen, here called I-DIRT (isotopic differentiation of interactions as random or targeted). Proteins with a light isotopic fraction greater than one standard deviation from a mean contaminant threshold (score of >0.7528 L/L+H) are scored as specific interactions and are included here. JCForrest@uams.edu Journal of virology 2014 imex curation Only protein-protein interactions imex curation o41974_mhv68 GAMMAHV.ORF73 73 protein protein mhv68 Murid herpesvirus 4 (MuHV-4) Murine gammaherpesvirus 68 MPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGTQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGPPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPYTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEKYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGSPGSSPGQQGQGSQT mint d0u1p1_9gama ORF52 protein protein Wood mouse herpesvirus Wood mouse herpesvirus MASKKPDKTYEEMVKEVERLKLENKSLKQKVKSSGAVPSDDSILTAAKRESIIVSSSRALGAVASRKIEAKVRSRAAKAVTEQELTTMLQSLTLRVDVSMEETTMGATGGIGPSSNTETKKRKPRSKSRHRHDHE hsp7c_mouse Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Hspa8 Hsc70 Hsc73 protein protein mouse Mus musculus Mouse MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD rl21_mouse 60S ribosomal protein L21 Rpl21 protein protein mouse Mus musculus Mouse MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRHAAPPREAHFVRTNGKEPELLEPIPYEFMA mint tra2b_mouse Transformer-2 protein homolog beta Silg41 Transformer-2 protein homolog B Splicing factor, arginine/serine-rich 10 Silica-induced gene 41 protein Tra2b Sfrs10 protein protein mouse Mus musculus Mouse MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRSRSYSPRRY mint Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing d0u1p6_9gama ORF57 protein protein Wood mouse herpesvirus Wood mouse herpesvirus MAQQMLEAGALDHMMEGLPSDFDFDTSDEEGELSDSPPAEESTGPVRDVVYEPDPMFDDPPPTPSPDIRSPSPKARKRALSPEIIHNSPLLRDTTKYEPATKRSYSYHPRRSPQRENPRQKLKRGTDSRRPNRWNQKNPKQYWSLKPLLDYSKIPRAEYKNAKLLVPTTGKLRPEFYTDRFVDAIIQNAARNCPVSEKAVSLKNIEESFKLLHSFFNSGINKEPWLSTRYFAIFNNGLVVLTHMLDEQMAWAYACLKHGRELPTDDILMSTSEKLSQQLVIKLMEVIKCIEKDGIFSRILKGVADAVCLKAQFLRGMITLKRTPCSLPMYTLFVYVLTIPTLRTRVIRDPLLTQCKDVVLKYQPGDCITLLKAALNCHQCNKDCDKCKYILDPLLGQTHRTKGVFFVCE actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF srsf6_mouse Serine/arginine-rich splicing factor 6 Srsf6 Sfrs6 Srp55 Pre-mRNA-splicing factor SRP55 Splicing factor, arginine/serine-rich 6 protein protein mouse Mus musculus Mouse MPRVYIGRLSYNVREKDIQRFFSGYGRLLEIDLKNGYGFVEFEDSRDADDAVYELNSKELCGERVIVEHARGPRRDRDGYSYGSRSGGGGYSSRRTSGRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYADAHKERTNEGVIEFRSYSDMKRALDKLDGTEINGRNIRLIEDKPRTSHRRSYSGSRSRSRSRRRSRSRSRRSSRSRSRSISKSRSRSRSRSKGRSRSRSKGRKSRSKSKSKPKSDRGSHSHSRSRSKDKYGKSRSRSRSRSPKENGKGDIKSKSRSRSQSRSHSPLPAPPSKARSMSPPPKRASRSRSRSRSRSRSSSRD ef1a1_mouse Elongation factor 1-alpha 1 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Eef1a1 Eef1a protein protein mouse Mus musculus Mouse MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK rl17_mouse 60S ribosomal protein L17 Rpl17 protein protein mouse Mus musculus Mouse MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLKKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMPRE h12_mouse Histone H1.2 H1-2 H1 VAR.1 H1c H1f2 Hist1h1c protein protein mouse Mus musculus Mouse MSEAAPAAPAAAPPAEKAPAKKKAAKKPAGVRRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGILVQTKGTGASGSFKLNKKAASGEAKPQAKKAGAAKAKKPAGAAKKPKKATGAATPKKAAKKTPKKAKKPAAAAVTKKVAKSPKKAKVTKPKKVKSASKAVKPKAAKPKVAKAKKVAAKKK srsf7_mouse Serine/arginine-rich splicing factor 7 Srsf7 Sfrs7 Splicing factor, arginine/serine-rich 7 protein protein mouse Mus musculus Mouse MRSSARGRPLQAATAFFLSLFFFLRRFERGFWLWGGDSETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPRRSRFDRPPARRPFDPNDRCYECGEKGHYAYDCHRYSRRRRSRSRSRSHSRSRGRRYSRSRSRSRGRRSRSASPRRSRSVSLRRSRSASLRRSRSGSIIGSRYFQSRSRSRSRSRSISRPRSSRSKSRSPSPKRSRSPSGSPHRSASPERMD o41925_mhv68 GAMMAHV.M3 M3 protein protein mhv68 Murid herpesvirus 4 (MuHV-4) Murine gammaherpesvirus 68 MAFLSTSVLIKCCILLLAGGLAESLTLGLAPALSTHSSGVSTQSVDLSQIKRGDEIQAHCLTPAETEVTECAGILKDVLSKNLHELQGLCNVKNKMGVPWVSVEELGQEIITGRLPFPSVGGTPVNDLVRVLVVAESNTPEETPEEEFYAYVELQTELYTFGLSDDNVVFTSDYMTVWMIDIPKSYVDVGMLTRATFLEQWPGAKVTVMIPYSSTFTWCGELGAISEESAPQPSLSARSPVCKNSARYSTSKFCEVDGCTAETGMEKMSLLTPFGGPPQQAKMNTCPCYYKYSVSPLPAMDHLILADLAGLDSLTSPVYVMAAYFDSTHENPVRPSSKLYHCALQMTSHDGVWTSTSSEQCPIRLVEGQSQNVLQVRVAPTSMPNLVGVSLMLEGQQYRLEYFGDH rl31_mouse 60S ribosomal protein L31 Rpl31 protein protein mouse Mus musculus Mouse MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN ro52_mouse E3 ubiquitin-protein ligase TRIM21 Trim21 Ro52 Ssa1 52 kDa Ro protein 52 kDa ribonucleoprotein autoantigen Ro/SS-A Ro(SS-A) Sjoegren syndrome type A antigen Tripartite motif-containing protein 21 RING-type E3 ubiquitin transferase TRIM21 protein protein mouse Mus musculus Mouse MSPSTTSKMSLEKMWEEVTCSICLDPMVEPMSIECGHCFCKECIFEVGKNGGSSCPECRQQFLLRNLRPNRHIANMVENLKQIAQNTKKSTQETHCMKHGEKLHLFCEEDGQALCWVCAQSGKHRDHTRVPIEEAAKVYQEKIHVVLEKLRKGKELAEKMEMDLTMQRTDWKRNIDTQKSRIHAEFALQNSLLAQEEQRQLQRLEKDQREYLRLLGKKEAELAEKNQALQELISELERRIRGSELELLQEVRIILERSGSWNLDTLDIDAPDLTSTCPVPGRKKMLRTCWVHITLDRNTANSWLIISKDRRQVRMGDTHQNVSDNKERFSNYPMVLGAQRFSSGKMYWEVDVTQKEAWDLGVCRDSVQRKGQFSLSPENGFWTIWLWQDSYEAGTSPQTTLHIQVPPCQIGIFVDYEAGVVSFYNITDHGSLIYTFSECVFAGPLRPFFNVGFNYSGGNAAPLKLCPLKM lana-orf52-1 1923201 N/A 1923204 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1923205 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2 mlana-mlana-1 1923201 N/A 1923204 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1923204 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 2 mlana_hsc70-1 1923201 N/A 1923204 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1923206 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 3B mlana-hspa8-1 1923202 N/A 1923204 unspecified role unspecified role bait bait purified purified c terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 1923206 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false 3A mlana-mlana-2 1923202 N/A 1923204 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 1923204 unspecified role unspecified role bait bait purified purified c terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 2 and 3A lana-multi-1 1923203 N/A 1923207 unspecified role unspecified role prey prey N/A 1923208 unspecified role unspecified role prey prey N/A 1923204 unspecified role unspecified role bait bait c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1923209 unspecified role unspecified role prey prey N/A 1923205 unspecified role unspecified role prey prey N/A 1923210 unspecified role unspecified role prey prey N/A 1923211 unspecified role unspecified role prey prey N/A 1923212 unspecified role unspecified role prey prey N/A 1923213 unspecified role unspecified role prey prey N/A 1923214 unspecified role unspecified role prey prey N/A 1923215 unspecified role unspecified role prey prey N/A 1923206 unspecified role unspecified role prey prey N/A 1923216 unspecified role unspecified role prey prey N/A 1923217 unspecified role unspecified role prey prey N/A 1923218 unspecified role unspecified role prey prey association association false false false Figure 1B and S3