UniProt UniProt http://www.uniprot.org ning-2014-1 Viral suppression of innate immunity via spatial isolation of TBK1/IKK{epsilon} from mitochondrial antiviral platform. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Ning Y.J., Wang M., Deng M., Shen S., Liu W., Cao W.C., Deng F., Wang Y.Y., Hu Z., Wang H. Virus - Publications including interactions involving viral proteins Origin of transfected host constructs according pmid 16281057 (following cited Lei et al 2010 pmid 21145761) h.wang@wh.iov.cn J Mol Cell Biol (1674-2788) 2014 imex curation Only protein-protein interactions ning-2014-5 Viral suppression of innate immunity via spatial isolation of TBK1/IKK{epsilon} from mitochondrial antiviral platform. chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunostaining immunostaining Ning Y.J., Wang M., Deng M., Shen S., Liu W., Cao W.C., Deng F., Wang Y.Y., Hu Z., Wang H. Virus - Publications including interactions involving viral proteins Origin of transfected host constructs according pmid 16281057 (following cited Lei et al 2010 pmid 21145761) h.wang@wh.iov.cn J Mol Cell Biol (1674-2788) 2014 imex curation Only protein-protein interactions ning-2014-2 Viral suppression of innate immunity via spatial isolation of TBK1/IKK{epsilon} from mitochondrial antiviral platform. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ning Y.J., Wang M., Deng M., Shen S., Liu W., Cao W.C., Deng F., Wang Y.Y., Hu Z., Wang H. Virus - Publications including interactions involving viral proteins Origin of transfected host constructs according pmid 16281057 (following cited Lei et al 2010 pmid 21145761) h.wang@wh.iov.cn J Mol Cell Biol (1674-2788) 2014 imex curation Only protein-protein interactions ning-2014-3 Viral suppression of innate immunity via spatial isolation of TBK1/IKK{epsilon} from mitochondrial antiviral platform. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Ning Y.J., Wang M., Deng M., Shen S., Liu W., Cao W.C., Deng F., Wang Y.Y., Hu Z., Wang H. Virus - Publications including interactions involving viral proteins Origin of transfected host constructs according pmid 16281057 (following cited Lei et al 2010 pmid 21145761) h.wang@wh.iov.cn J Mol Cell Biol (1674-2788) 2014 imex curation Only protein-protein interactions ning-2014-4 Viral suppression of innate immunity via spatial isolation of TBK1/IKK{epsilon} from mitochondrial antiviral platform. human-hep2 Homo sapiens hep2 hep2 confocal microscopy confocal microscopy immunostaining immunostaining Ning Y.J., Wang M., Deng M., Shen S., Liu W., Cao W.C., Deng F., Wang Y.Y., Hu Z., Wang H. Virus - Publications including interactions involving viral proteins Origin of transfected host constructs according pmid 16281057 (following cited Lei et al 2010 pmid 21145761) h.wang@wh.iov.cn J Mol Cell Biol (1674-2788) 2014 imex curation Only protein-protein interactions i0df37_9viru protein protein phlebovirus wch/97/hn/china/2011 phlebovirus wch/97/hn/china/2011 MSLSKCSNVDLKSVAMNANTVRLEPSLGEYPTLRRDLVECSCSVLTLSMVKRMGKMTNTVWLFGNPKNPLHQLEPGLEQLLDMYYKDMRCYSQRELSALRWPSGKPSVWFLQAAHMFFSIKNSWAMETGRENWRGLFHRITKSQKYLFEGDMILDSLEAIEKRRLRLGLPEILITGLSPILDVALLQIESLARLRGMSLNHHLFTSSSLRKPLLDCWDFFIPIRKKKTDGSYSVLDEDDEPGVLQGYPYLMAHYLNRCPFHNLIRFDEELRTAALDTIWGRDWPAIGDLPKEV tbk1_human Serine/threonine-protein kinase TBK1 TBK1 NAK TANK-binding kinase 1 T2K NF-kappa-B-activating kinase protein protein human Homo sapiens Human MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL ikke_human Inhibitor of nuclear factor kappa-B kinase subunit epsilon IKBKE IKKE IKKI KIAA0151 Inducible I kappa-B kinase protein protein human Homo sapiens Human MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH i0df37-tbk1-1 1540551 n/a 1540556 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540557 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 6A i0df37-ikbke 1540551 n/a 1540558 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540556 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 6A mavs-ikbke 1540551 n/a 1540559 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540558 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 7D Overexpression of SFTSV HA.tagged NSs (UniProt Ac I0DF37) impaired association i0df37-ikbke-3 1540552 n/a 1540556 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540558 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Supplem. 4 i0df37-tbk1-3 1540552 n/a 1540556 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region s tag s tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540557 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization false false false Supplem. 4 i0df37-tbk1-2 1540553 n/a 1540557 unspecified role unspecified role prey prey cell lysate cell lysate n/a 1540556 unspecified role unspecified role bait bait physical association physical association false false false 6B i0df37-ikbke-1 1540554 n/a 1540556 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1540558 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 7A i0df37-ikbke-2 1540555 n/a 1540558 unspecified role unspecified role neutral component neutral component n/a 1540556 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7C