HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html cook-2013-1 The Three Dimensional Structure and Interaction Studies of HCV p7 in DHPC by Solution NMR. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Cook G.A., Dawson L.A., Tian Y., Opella S.J. Virus - Publications including interactions involving viral proteins sopella@ucsd.edu Biochemistry (0006-2960) 2013 imex curation Only protein-protein interactions imex curation o92972-pro_0000278748 Protease NS2 protein protein hcvj4 Hepatitis C virus genotype 1b (strain HC-J4) (HCV) LDREMAASCGGAVLVGLVFLTLSPYYKVFLTRLIWWLQYFITRAEAHMQVWVPPLNVRGGRDAIILLTCAVHPELIFDITKLLLAILGPLMVLQAGITRVPYFVRAQGLIRACMLVRKVAGGHYVQMAFMKLGALTGTYVYNHLTPLRDWAHAGLRDLAVAVEPVVFSAMETKVITWGADTAACGDIILGLPVSARRGKEIFLGPADSLEGQGWRLL 810 1026 o92972-pro_0000278747 Viroporin p7 protein protein hcvj4 Hepatitis C virus genotype 1b (strain HC-J4) (HCV) ALENLVVLNAASVAGAHGILSFLVFFCAAWYIKGRLAPGAAYAFYGVWPLLLLLLALPPRAYA 747 809 amantadine amantadine 1-adamantanamine 1-adamantylamine 1-aminoadamantane amantadina amantadine Amantadine amantadinum Amantidine Aminoadamantane tricyclo[3.3.1.1(3,7)]decan-1-amine tricyclo[3.3.1.1(3,7)]decan-1-ylamine tricyclo[3.3.1.1(3,7)]decane-1-amine Viregyt Virosol adamantan-1-ylamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) DKNWSYNQZKUICI-UHFFFAOYSA-N n-nonyldeoxynojirimycin N-Nonyldeoxynojirimycin NN-DNJ small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 5-(n,n-hexamethylene)amiloride 5-(N,N-hexamethylene)amiloride HMA small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p7-ns2-1 1457953 n/a 1457954 unspecified role unspecified role neutral component neutral component 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a P7 1457955 unspecified role unspecified role neutral component neutral component 1h label tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 7A-D Ala12 and Val15 of NS2 are required for interaction. p7-ns2-2 1457953 n/a P7 1457955 unspecified role unspecified role neutral component neutral component O92972-PRO_0000278747:p.Trp48Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a NS2 1457954 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Fig 7E-H amantadine-p7-1 1457953 n/a Amantadine 1457956 unspecified role unspecified role neutral component neutral component n/a P7 1457955 unspecified role unspecified role neutral component neutral component 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 6A nn-dnj-p7-1 1457953 n/a P7 1457955 unspecified role unspecified role neutral component neutral component 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457957 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Fig 6B hma-p7-1 1457953 n/a 1457958 unspecified role unspecified role neutral component neutral component n/a P7 1457955 unspecified role unspecified role neutral component neutral component 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 6C