HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html vogt-2013-4 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Vogt D.A., Camus G., Herker E., Webster B.R., Tsou C.L., Greene W.C., Yen T.S., Ott M. Virus - Publications including interactions involving viral proteins mott@gladstone.ucsf.edu PLoS Pathog. (1553-7366) 2013 imex curation Only protein-protein interactions imex curation vogt-2013-1 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vogt D.A., Camus G., Herker E., Webster B.R., Tsou C.L., Greene W.C., Yen T.S., Ott M. Virus - Publications including interactions involving viral proteins mott@gladstone.ucsf.edu PLoS Pathog. (1553-7366) 2013 imex curation Only protein-protein interactions imex curation vogt-2013-2 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vogt D.A., Camus G., Herker E., Webster B.R., Tsou C.L., Greene W.C., Yen T.S., Ott M. Virus - Publications including interactions involving viral proteins mott@gladstone.ucsf.edu PLoS Pathog. (1553-7366) 2013 imex curation Only protein-protein interactions imex curation vogt-2013-3 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vogt D.A., Camus G., Herker E., Webster B.R., Tsou C.L., Greene W.C., Yen T.S., Ott M. Virus - Publications including interactions involving viral proteins mott@gladstone.ucsf.edu PLoS Pathog. (1553-7366) 2013 imex curation Only protein-protein interactions imex curation vogt-2013-5 Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Vogt D.A., Camus G., Herker E., Webster B.R., Tsou C.L., Greene W.C., Yen T.S., Ott M. Virus - Publications including interactions involving viral proteins mott@gladstone.ucsf.edu PLoS Pathog. (1553-7366) 2013 imex curation Only protein-protein interactions imex curation plin3_human Perilipin-3 Cargo selection protein TIP47 47 kDa mannose 6-phosphate receptor-binding protein Placental protein 17 PLIN3 TIP47 M6PRBP1 Mannose-6-phosphate receptor-binding protein 1 protein protein human Homo sapiens Human MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQVLSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK q9wmx2-pro_0000037551 Non-structural protein 5A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYKGVWRGDGIMQTTCPCGAQITGHVKNGSMRIVGPRTCSNTWHGTFPINAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFLVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFEPLQAEEDEREVSVPAEILRRSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLSESTVSSALAELATKTFGSSESSAVDSGTATASPDQPSDDGDAGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCC 1973 2419 q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 ns5a-tip47-6 1455185 n/a TIP47 1455190 unspecified role unspecified role neutral component neutral component n/a NS5A 1455191 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5C, 5E and 5F ns5a-tip47-2 1455185 n/a TIP47 1455190 unspecified role unspecified role neutral component neutral component n/a NS5A 1455192 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5D, 5E and 5F ns5a-tip47-1 1455186 n/a NS5A 1455191 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a TIP47 1455190 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A and 1B ns5a-tip47-4 1455187 n/a TIP47 1455190 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a NS5A 1455191 unspecified role unspecified role bait bait Q9WMX2-PRO_0000037551:p.[Trp9Ala;Ile12Val] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1D and 3E ns5a-tip47-5 1455188 n/a TIP47 1455190 unspecified role unspecified role bait bait n/a NS5A 1455191 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1E ns5a-tip47-3 1455189 n/a NS5A 1455191 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9WMX2-PRO_0000037551:p.Trp9Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a TIP47 1455190 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1C