HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html li-2013-15 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-14 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins The identifier gi|545835 is for a bovine protein with 97% homology to F1SBL0 (pig Phosphoinositide phospholipase C). gi|4929901 is an E.coli protein with low homology to any mammalian protein so has not been added. huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-16 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-17 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-18 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation li-2013-19 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunostaining immunostaining Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ., Li D., Dong H., Li S., Munir M., Chen J., Luo Y., Sun Y., Liu L., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2013 imex curation Only protein-protein interactions imex curation q5u8x5_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYALSSTNEIGLLGAGGLTTTWEEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV hbb_pig Hemoglobin subunit beta HBB Beta-globin Hemoglobin beta chain protein protein pig Sus scrofa (Pig) Pig MVHLSAEEKEAVLGLWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSNADAVMGNPKVKAHGKKVLQSFSDGLKHLDNLKGTFAKLSELHCDQLHVDPENFRLLGNVIVVVLARRLGHDFNPNVQAAFQKVVAGVANALAHKYH ddx58_pig Antiviral innate immune response receptor RIG-I DDX58 DEAD box protein 58 RHIV-1 RIG-I-like receptor 1 RNA helicase induced by virus Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein Probable ATP-dependent RNA helicase DDX58 protein protein pig Sus scrofa (Pig) Pig MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENSDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFSYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEKCHFTVVGDAFRERFVSKLHPKPKSFGNIEKRAKIYCARPDCSHDWGIYVRYKAFEMPFIKIESFVVEDIATGVQTVHAKWKDFNFEKLSFDAAEMAGGAQDLGLQGMGN f1sbl0_pig 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase PLCB4 protein protein pig Sus scrofa (Pig) Pig MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEAVGKSENDLEGRIVCVCSGTDLVNISFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSVDDLGHKEAIANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNRLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELSSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEIRDLHLSQQCELLRKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV hbt_pig Hemoglobin subunit theta Hemoglobin theta chain Theta-globin protein protein pig Sus scrofa (Pig) Pig VHFTAEEKSVITGLWGKVNVEETGGEAVGRLLVVYPWTQRFFDSFGNMSSPSAIMGNPKVKAHGKKVLTSFGDAVKNMDNLKGTFAKLSELHCDKLHVDPENFRLLGNMIVIILASHFGGEFTPEVQAAWQKLVAGVATALAHKYH capsid-hb-1 2857888 N/A 2857894 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false core region binding region binding-associated region binding region range range range range false N/A 2857895 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2A and 3 capsid-ddx58-1 2857888 N/A 2857896 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857894 unspecified role unspecified role bait bait capsid region binding region binding-associated region binding region range range range range false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 8A capsid-multi-3 2857889 N/A 2857897 unspecified role unspecified role prey prey pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 N/A 2857894 unspecified role unspecified role bait bait capsid region binding region binding-associated region binding region range range range range false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2857898 unspecified role unspecified role prey prey pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 N/A 2857895 unspecified role unspecified role prey prey pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 association association false false false 1A and table 2 hbb-capsid-1 2857890 N/A 2857894 unspecified role unspecified role bait bait n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false capsid region binding region binding-associated region binding region range range range range false ecoli Escherichia coli (strain K12) N/A 2857895 unspecified role unspecified role prey prey n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false 2B core-hbb-1 2857891 N/A 2857894 unspecified role unspecified role bait bait core region binding region binding-associated region binding region range range range range false N/A 2857895 unspecified role unspecified role prey prey physical association physical association false false false 2C hbb-q5u8x5_9flav-1 2857892 N/A 2857894 unspecified role unspecified role neutral component neutral component c terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false capsid region binding region binding-associated region binding region range range range range false N/A 2857895 unspecified role unspecified role neutral component neutral component n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 2D hbb-q5u8x5_9flav-2 2857893 N/A 2857894 unspecified role unspecified role neutral component neutral component capsid region binding region binding-associated region binding region range range range range false N/A 2857895 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2E