HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html kang-2010-9 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Journal of virology 2010 imex curation Only protein-protein interactions imex curation kang-2010-8 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Journal of virology 2010 imex curation Only protein-protein interactions imex curation kang-2010-12 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Re-IP. Protein complexes were re-immunoprecipitated from the FLAG elutes using anti-myc antibody and detected for the presence of ERK2-EGFP by Western blotting Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Journal of virology 2010 imex curation Only protein-protein interactions imex curation mk01_mouse Mitogen-activated protein kinase 1 Mapk1 Erk2 Mapk Prkm1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 protein protein mouse Mus musculus Mouse MAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNLNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS g3g926_9alph US2 protein protein pseudorabies virus pseudorabies virus MGVTAITVVTLMDGSGRIPAFVGEAHPDLWKVLTEWCYASLVQQRRAADEDSPRQHVVLRSSEIAPGSLALLPRATRPVVRTRSDPTAPFYITTETHELTRRPPADGSKPGEPLRISPPPRLDTEWSSVLNGIQYLNSGARGTAPVHLWILGAADLCDQVLLAASRSTAAGAPGAPTGARLTRRRPGLTDADALDVIVAGIPATRAMFARVHNRSWRHAGEWTEALHAQIVTRGDVRRRRGGRGNGRERAPRCTIS mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV mapk1-us2-1 1933867 N/A 1933870 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1933871 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2C us2-us2-1 1933867 N/A 1933871 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1933871 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false self interaction self interaction false false false 3B us2-mapk1-1 1933867 N/A 1933871 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1933871 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 1933870 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false 3C us2-erk2-2 1933867 N/A 1933870 unspecified role unspecified role prey prey P63085:p.[Asp316Pro;Asp319Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1933871 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4C us2-mapk1-2 1933867 N/A 1933871 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1933870 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3C us2-erk2-1 1933868 N/A 1933870 unspecified role unspecified role prey prey N/A 1933871 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false 2B erk2-us2-2 1933868 N/A 1933870 unspecified role unspecified role prey prey P63085:p.[Asp316Ala;Asp319Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P63085:p.Tyr261Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P63085:p.Asn236Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P63085:p.His230Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false N/A 1933871 unspecified role unspecified role bait bait physical association physical association false false false 4B map2k1-mapk1-3 1933868 N/A 1933872 unspecified role unspecified role bait bait Q02750:p.Lys97Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 1933870 unspecified role unspecified role prey prey P63085:p.His230Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P63085:p.Tyr261Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P63085:p.Asn236Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P63085:p.[Asp316Ala;Asp319Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false amino acid number is different from the one in the publication physical association physical association false false false 4B mapk1-us2-2 1933868 N/A 1933870 unspecified role unspecified role prey prey N/A 1933871 unspecified role unspecified role bait bait G3G926:p.Arg184_Arg185delinsLysLys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false G3G926:p.Arg184_Arg185delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false G3G926:p.Arg183_Arg185delinsAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false G3G926:p.Leu181_Arg185delinsAlaAlaAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2E us2-mapk1-3 1933869 N/A 1933871 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1933871 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1933870 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false 3C ERK2-EGFP was immunoprecipitated specifically in the complexes containing oligomeric Us2.