MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint fiedler-2008a-1 Identification and characterization of RHOA-interacting proteins in bovine spermatozoa. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Fiedler SE., Bajpai M., Carr DW. Virus - Publications including interactions involving viral proteins Biol. Reprod. (0006-3363) 2008 fiedlers-2008-1 Identification and characterization of RHOA-interacting proteins in bovine spermatozoa. bovine-spermatozoa Bos taurus spermatozoa spermatozoa coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Fiedler SE., Bajpai M., Carr DW. Virus - Publications including interactions involving viral proteins Biol. Reprod. (0006-3363) 2008 fiedlers-2008-2 Identification and characterization of RHOA-interacting proteins in bovine spermatozoa. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Fiedler SE., Bajpai M., Carr DW. Virus - Publications including interactions involving viral proteins Biol. Reprod. (0006-3363) 2008 rop1l_bovin Ropporin-1-like protein ROPN1L protein protein bovin Bos taurus (Bovine) Bovine MPLPDTMFCAQQIHIPPELTDILKQFTKAAIRTQPADVLQWSAGYFSALSRGDPLPVKDRIEMPMATQKTDTGLTQGLLKVLHKQCSHKEYVDLADLEQKWKNLCLPVEKFRALLQLDPCEDKIEWIKFLALGCSMLGGSLNTAMKHLCEILTTDPEGGPARIPFGTFSYVYRYLSGLDSDIPESDTEAYLSSLKENAAARKNGMIGLSDFFVLKRKI mint akap3_bovin A-kinase anchor protein 3 AKAP3 AKAP110 Protein kinase A-anchoring protein 3 A-kinase anchor protein 110 kDa protein protein bovin Bos taurus (Bovine) Bovine MSDRVDWLQSQNGVCKVDVYSPGDSQPQDWKMSDESLSIFKEASHDPIRVLSWLRRDLEKSTAGFQDIRFKPGESSLGREMVSSGDPRKGFCVDYYNTTSRGSPGRLHFEMSHRENPHQGPTCNGSSVDEVSFYANRLTNLVIAMARKEINEKIDGSENRCVHQSVYMGDEPPPNKSLSKVASELVNETVTACSKNTPSDKAPGSGDRASGTLQSPPNLKYKSTLKIKESNKGGRGPDDRPSSKKSFFYKEVFESRNAGDAKEGGRTLPAERKMFRGYERPDDFTASISQGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATIVLKKVLIKHAKEVVSDLIDSFMKNLHNVTGTLMTDTDFVSAVKRSFFSHGSQKATDIMDAMLGKLYSVIFAKKPPETVRKTKDKSESYSLVSMKGMGDPKHRNVNFASMKSEGKVRERVCTPTPKPEKSCVETLGEHIIKEGLTLWHNSQQKEGISACLQGSPFVTPKRQYKPVPDFPLGFPFDPCNFSLPMQCPEKPENFMCDSDSWAKDLLVSALLLIQYHLAQGGNMDAQSFLEAAGTSNLYPTKSPAVSHESSLRSPQVGADPEEVEKKDLMSVFFNLIRNLLSETIFKSDHSCDPKATKEDNSPQCERPVTPSPAKLNECDETGGAFAGLTKMVANKLDGHMNGQMVDHLMDSVMKLCLIIAKSCDSPLAELGDDKSGDASRPTSAFPESLYECLSTKGTGTAETLLQNAYQAIHNELRSLSAQPPEGCTAPKVIVSNHNLTDTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSAAAVEKGRSVGEVLQSVLRYEKERQLDEAVGNVTRLQLLDWLMVNL May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction mint fscb_bovin Fibrous sheath CABYR-binding protein FSCB protein protein bovin Bos taurus (Bovine) Bovine MEEKDESEQSISAGRQEIRKRRRPSQPMVDKSQQTEVTEKKKQLSIPQSSGPKAALSIGNIPGSKLNYECHRVSSQLQQTWIKRKRVQDMADKSLQTETIAEEKKEEIKLVCEAVVPEEKPAAVEVGPEFPESVREVEVPPNRYSVQVKIDRSQQTTCTGDWTMMNFPQKEKLDKEQQTYFSESEIVVIGWPTNSFSKSKEGAQKRKSSGNIFLSEHPEFQPTTSSNEEIRRPSISRTVSISPTKKDSPVPLEDEKDVPVEVQPPAAEEISAEEQLPLAETTSEEVPVAVQPPPAEEPPLEAQPSPTEEAPGDEAPAKVEPTPAEEALSEKPPAEEALVEIQPSPVEEAAGDEAPAKVEATSSEETLLKEPLTEVQPPAAEEAPIQDTPELQLSPAVEAPAEEAPAEAEPPPAEEAPAEEAPEVQSPPAEEAPAEEPPEIQSPPAEEAPAEEPPEVQSPPAEEAPAEEAPEVQSPPAEEAPAEEPPEVQSPPAEEVPAGEPPEVQSPPAEEAPAEEAPEVQSPPAEEVPAEEALAEVEPPPAEEAPAGEPSEVQSPPAEEAPAEEAPEVQSPPSEEAPAEEAPEVQSVPAGQAPAEEPLEVQPPPAEDATEEEASEVQSLPTDEAPAEEGLGFQSPPADKAPEEEAPEVQSPPAEEAPAEEPPEVQSLPADEAPAEEATEEVQSPPTEESPAEEAPAELQPPSTEETTSEMVSVEKQPSLTEEPFITPISLEETSAEVLLPPFEQTPADEALVENVSPVDQAPKEADVLVEKLESGNLDDKPKSEEPLERDTIPKDSSGTKNEGVSFEIKGVIHIELE May be involved in the later stages of fibrous sheath biogenesis. Binds calcium mint rop1_bovin Ropporin-1 ROPN1 Rhophilin-associated protein 1 protein protein bovin Bos taurus (Bovine) Bovine MPQTDKQICIPPELPELLKQFTKAAIRSQPQDLIQWAAEYFGAMSRGEIPPVRERSERVALSNWAELTPELLKILHSRVGGRLIVQADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDSGPPRIPFSTFQFLYTYIAEVDGEISASHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE mint q3sys3_bovin Sperm autoantigenic protein 17 SPA17 Sperm surface protein Sp17 protein protein bovin Bos taurus (Bovine) Bovine MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDPAEWGAKVDDRFYNNHAFKEQESPEKHEPGKENSQTSVKEKTALESPEEDKDMEENAALKIQAAFRGHLAREEVKKMKSSDLEEEKTEKNE mint q5w5u3_bovin Hexokinase HK1 protein protein bovin Bos taurus (Bovine) Bovine MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQHVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS mint rhoa_bovin Transforming protein RhoA RHOA ARHA RHO12 Gb p21 protein protein bovin Bos taurus (Bovine) Bovine MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL mint Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1 p79343_bovin Acrosin bovine proacrosine protein protein bovin Bos taurus (Bovine) Bovine MRIIGGQDAAHGSWPWMVSLQIFTYHNNRRYHVCWGLLLNAHWLLTAAHCFRIKKKVTDWRLIFGAKEVEWGSNKPVKPPLQERYVEKIIIHEKYSASSEANDIALIKITPPVICGHFIGPGCLPQFRAGPPRVPQTCWVAGWGFLRENARRTSPVLQEAHVDLIDLDLCNSTRWYNGRIRSTNVCAGYPEGKIDTCQGDSGGPLMCKDSVENSYVVVGITSWGVGCSRAKRPGVYTSTWSYLNWIASKIGSNTVHMIQLPTAPPASTPAAQASPGSVQPSIRPPWFFQHVPQPPPSQQAIAVAQPSRPSSPRPSVPPAPRPPRPPPPQPSTRPPQALSFAKRLQQLIEVLKGKTFLNEKSNYEMETTGLPGQHASS mint tba4a_bovin Tubulin alpha-4A chain TUBA1 TUBA4A Tubulin alpha-1 chain Alpha-tubulin 1 protein protein bovin Bos taurus (Bovine) Bovine MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE mint Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain tbb2b_bovin Tubulin beta-2B chain TUBB2B protein protein bovin Bos taurus (Bovine) Bovine MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA mint Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain q3zby4_bovin Fructose-bisphosphate aldolase ALDOC protein protein bovin Bos taurus (Bovine) Bovine MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACAIKYSPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASLNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQQDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAVQSLYIANHAY mint odfp1_bovin Outer dense fiber protein 1 ODF1 ODFP protein protein bovin Bos taurus (Bovine) Bovine MAALSCLLDSVRRDIKKVDRELRQLRCIDELSARCLCDLYMHPYCCCDLHPYPYCLCYSKRSRSCGLCDLYPCCLCDVKLYCLRPSLRSLERKAIRAIEDEKRELAKLRRTTNRILASSCCSSNILGSVNVCGFEPDQVKVRVKDGKVCVSAERENRYDCLGSKKYSYMNICKEFSLPPCVDEKDVTYSYGLGSCVKIESPCYPCTSPCNPCNPCNPCSPCSPCNPCNPCSPCSPCSPCNPCDPCNPCYPCGSRFSCRKMIL Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail mint ace_bovin Angiotensin-converting enzyme ACE DCP1 Dipeptidyl carboxypeptidase I Kininase II protein protein bovin Bos taurus (Bovine) Bovine MGAASGRRSPPLLLPLLLLLLPPPPVILELDPALQPGNFPADEAGAQIFAASFNSSAEQVLFQSTAASWAHDTNITEENARLQEEAALLSQEFSEAWGQK fragmentation type: fragment mint Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin q9xs94_bovin protein protein bovin Bos taurus (Bovine) Bovine MSDDIDWLHSRRGVCKVDLYSPRGQQDQDRKVICFVDVSTLSMEDKDSKDAAGCSSEGDLNLENLEEKEIIVIKDTEKQDQSKTEGSVCLFKQAPSDPLSVFNWLLNDLQKYALGFQHALSPSASSCKHKVGETEGNCQKLSSGNCYSIYANHLNMDCVNNEPQSLRLEMTAAKNTNNNQSPSTPPGKSPSNQRAVISPDGECSMDDLSFYVNRLSSLVIQMARKEIKEKLENGSKCLHHSIYPPSGDKGKNSPRSAVSKIASEMAHDAVEVTSAEMRGTGEECRDGGRKTFLYSELSNKNKGGDKQMCQRDSKDFADSISKGLMVYANQVASDMMVSVMKTLKVHSSGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNHGKQNAADIMEAMLKRLVSALLGEKKETKSQCLSYTSLKAGSHDAKCKNQSLEFSSVKAEMKGKDKCKVKQEECKSLTSAEKVSEHILKESLTMWNQKQCNQGKMPSKTCPHKEEKREKISPSTDSLAKDLIVSALMLIQYHLTQQAKGKEACEDDCPPGTTSYMTQSAQYEKCGGSQSAKALSMKHLETRGAPGPSTSMKDNQHLDSQKLDMSNMVLSLIQKLLNESPFNCEDLCEGENKHSEIRTNKSTSIFKKSDREEEQGQDNREVDFVGGMKQVNRQFIDQLVESVMKLCLIMAKYSNNEAALTDLEEQASSSNNSNFYQTSGPRSSYEAGMSQSYQDSHGPEVIVNNQCSTSSLQKQLQAVLQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAAEKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQLLDWLLGNL mint kap2_bovin cAMP-dependent protein kinase type II-alpha regulatory subunit PRKAR2A protein protein bovin Bos taurus (Bovine) Bovine MSHIQIPPGLTELLQGYTVEVLRQRPPDLVDFAVDYFTRLREARSRASTPPAAPPSGSQDFDPGAGLVADAVADSESEDEEDLDVPIPGRFDRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCRLQEACKDILLFKNLDPEQLSQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKVYKDGERIITQGEKADSFYIIESGEVSILIKSKTKVNKDGENQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSMDLIDPGQ mint akap3-ropn1l 793700 rop1l_bovin ROPN1L 793703 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false akap3_bovin AKAP3 793704 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F3A mint akap3-fscb 793700 akap3_bovin AKAP3 793704 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false fscb_bovin CABYR 793705 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false F3B F5 mint akap3-ropn1-1 793700 rop1_bovin ROPN1 793706 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false akap3_bovin AKAP3 793704 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F3C mint akap3-spa17 793700 q3sys3_bovin SPA17 793707 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false akap3_bovin AKAP3 793704 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F3D mint rhoa-ace 793701 q5w5u3_bovin Hexokinase 1 isoform HKIR 793708 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level rhoa_bovin RHOA 793709 predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level p79343_bovin Proacrosin 793710 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tba4a_bovin Tubulin alpha-2/alpha-4 chain 793711 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tbb2b_bovin Beta tubulin 793712 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level q3zby4_bovin Aldolace C 793713 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level odfp1_bovin Outer dense fiber of sperm tails 2 isoform 1 793714 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level ace_bovin Angiotensine-converting enzime 793715 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level q9xs94_bovin AKAP4 793716 fingerprinting peptide massfingerprinting fingerprinting unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false T1 homomint mint akap3-prkar2a 793702 kap2_bovin PRKAR2A 793717 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified akap3_bovin AKAP3 793704 predetermined predetermined participant predetermined unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association false false false F4 mint