DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chang-2005-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang CF., Huang TH. Virus - Publications including interactions involving viral proteins bmthh@gate.sinica.edu.tw FEBS letters 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chang-2005-5 in vitro In vitro light scattering light scattering experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang CF., Huang TH. Virus - Publications including interactions involving viral proteins bmthh@gate.sinica.edu.tw FEBS letters 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chang-2005-6 in vitro In vitro crosslink cross-linking study crosslink experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang CF., Huang TH. Virus - Publications including interactions involving viral proteins bmthh@gate.sinica.edu.tw FEBS letters 2005 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid n-64 3716400 N/A 3716403 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified 281-365 binding region 281-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 248-365 binding region 248-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig3a, Fig 4c n-65 3716401 N/A 3716403 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified 281-365 binding region 281-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 248-365 binding region 248-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig3a, Fig 4c n-66 3716402 N/A 3716403 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 281-365 binding region 281-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3716403 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified 281-365 binding region 281-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 248-365 binding region 248-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 4b