IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2004-7 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus liu-2004-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus liu-2004-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus liu-2004-4 in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus liu-2004-5 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus liu-2004-6 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel experimental particp experimental participant identification experimental particp Liu S., Xiao G., Chen Y., He Y., Niu J., Escalante CR., Xiong H., Farmar J., Debnath AK., Tien P., Jiang S. Virus - Publications including interactions involving viral proteins SJiang@NYBloodcenter.org Lancet (London, England) 2004 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT s-s-10 3725989 N/A 3725995 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 N/A 3725995 unspecified role unspecified role neutral component neutral component biotin tag biotin tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 direct interaction direct interaction false false false Figure 5, Table 3 s-s-11 3725989 N/A 3725995 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity unspecified role unspecified role neutral component neutral component biotin tag biotin tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 direct interaction direct interaction false false false Figure 5, Table 3 s-26 3725990 N/A 3725995 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 direct interaction direct interaction false false false Figure 6 s-s-12 3725990 N/A 3725995 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 N/A 3725995 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 direct interaction direct interaction false false false Figure 6 s-s-6 3725991 N/A 3725995 unspecified role unspecified role prey prey NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725995 unspecified role unspecified role bait bait CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3725991 2, bottom. s-s-7 3725992 N/A 3725995 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725995 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3, top. s-s-8 3725993 N/A 3725995 unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725995 unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 4, top s-s-9 3725994 N/A 3725995 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component NP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725995 weight by comassie molecular weight estimation by coomasie staining weight by comassie tag perox activity tag visualisation by peroxidase activity tag perox activity unspecified role unspecified role neutral component neutral component CP1_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 4, middle and bottom