HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html taylor-1999-2 Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Science (0036-8075) 1999 imex curation Only protein-protein interactions imex curation taylor-1999-1 Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. in vitro In vitro pull down pull down tag visualisation tag visualisation Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Science (0036-8075) 1999 imex curation Only protein-protein interactions imex curation taylor-1999-4 Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Science (0036-8075) 1999 imex curation Only protein-protein interactions imex curation e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC p27958-pro_0000037570 Envelope glycoprotein E2 protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) ETHVTGGNAGRTTAGLVGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGCPERLASCRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGNNTLLCPTDCFRKYPEATYSRCGSGPRITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACNWTRGERCDLEDRDRSELSPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSSIASWAIKWEYVVLLFLLLADARVCSCLWMMLLISQAEA 384 746 pkr-2 1434706 n/a PKR 1434709 putative self putative self putative self putative self human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation false true false Fig. 2C pkr-3 1434706 n/a PKR 1434709 putative self putative self putative self putative self ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false Fig. 2D eif2ak2-p27958_pro-1 1434706 n/a PKR 1434709 putative self putative self putative self putative self ecoli Escherichia coli (strain K12) n/a 1434710 inhibitor inhibitor unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation false false false Fig. 2D P27958-PRO_0000037570 decreases activity of P19525 e2-pkr-1 1434707 n/a E2 1434710 unspecified role unspecified role prey prey region mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a PKR 1434709 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2A e2-pkr-5 1434708 n/a E2 1434710 unspecified role unspecified role neutral component neutral component n/a PKR 1434709 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3H E2L (E2 with the leader peptide)